Ranca细胞系—小鼠肾癌细胞系哪家提供 货号:GD-C16319721
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞系—说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞系—就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞系—肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞系—刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Propanol
Disperse Blue26
4-Nitro-3-(trifluoroMethyl)benzonitrile
6-Methoxypicolinic acid
2',3'-O-Isopropylideneadenosine
1-(TERT-BUTYL)-3,5-DIMETHYL-1H-PYRAZOLE-4-CARBALDEHYDE
1-Isocyanato-4-methyl-2-nitrobenzene
3,5-Dibromo-6-methylpyridin-2-amine , 97%
5-Bromo-2-methoxypyrimidine
N-(4-Fluorophenyl)maleamic acid
2-Hydroxy-2-methylbutyric Acid
Hexachlorocyclopentadiene
4-(Chloromethyl)tolunitrile
Citric acid
(S)-(-)-Indoline-2-carboxylic acid
2-BroMo-3-nitrobenzoic acid
(S)-N-(2,6-DiMethylphenyl)piperidine-2-carboxaMide
4-DifluoroMethoxy-3-hydroxybenzaldehyde
Methyl 2-fluorophenylacetate, 99%
1,2-DIPHENYLHYDRAZINE
Butopyronoxyl
Triethoxysilane
Ethyl isobutyrylacetate
Hexafluoro-2-methylisopropanol
Benzalphthalide
5-METHYLNICOTINAMIDE
5-(2-NITROPHENYL)-2-FURANCARBOXYLIC ACID
3,4-Difluoro-N-(4-fluorobenzyl)aniline, 97%
2-Methyl-3,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3-d]pyriMidine-4-thione, 96%
5-Methyl-2-(4-Methylphenyl)benziMidazole, 95%
Ferrous sulfide
2,3,6-TRIFLUOROBENZYL BROMIDE
4-(TrifluoroMethyl)propiophenone, 97%
Nepsilon-(tert-Butoxycarbonyl)-Nalpha-[(9H-fluoren-9-ylMethoxy)carbonyl]-D-lysine , 98%
2,4-DiaMinobenzenesulfonic acid
Methyl acetate
N-Methyl-4-(trifluoromethoxy)aniline 97%
N-(4-methylbenzyl)butan-1-amine(SALTDATA: HCl)
Acetone 2,4-Dinitrophenylhydrazone
(S)-1-Cbz-3-N-Boc-Aminopiperidine
1-ETHOXYNAPHTHALENE
4-Chloro-3-nitrobenzenesulfonic acid
4-Bromobenzylamine
N-PROPYLAMINE HYDROCHLORIDE
2,4,6-TribroMopyriMidine
Bis(carbonyldithio)tetrathiafulvalene
2-BroMo-6-trifluoroMethylpyridine-4-boronic acid, pinacol ester
Adenine sulfate, 98%, synthetic
2,2,6,6-TetraMethyltetrahydro-4(1H)-pyridinone, 97%
Meso-1,2,3,4-TetrabroMobutane
Diethyl 2,5-Dioxahexanedioate
4-Thiouracil
methyl 5-nitro-6-oxo-1,6-dihydropyridine-3-carboxylate
ethyl 2-chloropyrimidine-5-carboxylate
(2-hydroxypropyl)trimethylammonium formate
FMOC-(R)-3-AMINO-3-(4-BROMO-PHENYL)-PROPIONIC ACID
N-Boc-1,2,5,6-tetrahydropyridine-4-boronic acid pinacol ester
CYCLOOCTANONE
6-BROMO-2-METHYLPYRIDIN-3-YLBORONIC ACID
1-(MethoxyMethoxy)naphthalene , 97%
Difluoroiodomethane 95%
2-Methyl-2-(1-methylpropyl)-1,3-propanediol Dicarbamate
Methyl 2-Methoxy-5-(methylsulfonyl)benzoate
4,5-Methylenedithio-1,3-dithiol-2-one
SPERMINE TETRAHYDROCHLORIDE
Germanium oxide
ETHYL PIPECOLINATE
4-Aminoindole
1-Phenyl-1-cyclopropanecarboxylic acid
2,3-DibroMo-6,7-dicyanonaphthalene
2-(4-BroMophenyl)-2-Methylpropanoic acid
SodiuM chloride, 99+%, ACS reagent
L-MethioninaMide Hydrochloride
Dipropyl 2,5-Pyridinedicarboxylate
1H-Pyrazole-3-carboxylicacid, 5-methyl-
3-Phenylpiperidine
2,5-Dihydroxy-1,4-benzoquinone
Cinnamyl acetate
Porcine HSP-70/HSPA9 (Heat Shock Protein 70) ELISA Kit
Flt3L ELISA Kit
Human hnRNP D ELISA Kit
Human DRD2 ELISA Kit
cAMP ELISA Kit
Rat Interleukin 13,IL-13 ELISA Kit
Chicken major histocompatibility complex,MHC/B ELISA Kit
PR3AB ELISA Kit
PDGFC ELISA Kit
TC ELISA Kit
Modified CCDA Medium Base
Modified CCDA Medium Base
LUTETIUM ACETATE
trans-^b-Nitrostyrene, 98%
4-Butylaniline
2-PhenoxyethylaMine, 98%
Triethoxysilane
acetyl-2-13C chloride
N-Benzyl-N-Cyclopropylamine
2,6-Naphthalene Disulfonic Acid
1-Aminobenzotriazole
9-METHOXY-9-BORABICYCLO[3.3.1]NONANE
4-(trans-4'-n-Pentylcyclohexyl)cyclohexanone
StrontiuM lanthanuM sulfide
1-BroMo-4-(trifluoroMethoxy)benzene, 98%
3,5-DiMethyl-1-hexyn-3-ol
2-Chloro-5-iodobenzoic Acid
(S)-(-)-1-(4-Fluorophenyl)ethylaMine, ChiPros 99%, ee 99%
1,5-DIPHENYL-1,5-PENTANEDIONE
2-HydroxybenziMidazole-5-boronic acid, pinacol ester
4-aMinohexanoylphenone
3-Chloro-4-hydroxyphenylacetic Acid
2,5-Dimethylfuran, 99%
tert.-Butyl methyl malonate, 95%
Methyl 2,5-Dibromobenzoate