SW 1353细胞系—人软骨肉瘤细胞系哪家提供 货号:GD-C16319113
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,6-Dichloro-4-iodopyridine, 97%
3-Indoleacrylic acid
METHYL 3-ETHOXYTHIOPHENE-2-CARBOXYLATE
(S)-(+)-1-Phenyl-1,2-ethanediol
1,1'-CARBONYLDIPIPERIDINE
(Diacetoxyiodo)benzene
2,5-Diphenyloxazole
(6-Aminopyridin-3-yl)methanol ,97%
2,8-diazaspiro[4.5]decan-3-one(SALTDATA: 2HCl)
Ethyl 2-Cyano-3,3-diphenylacrylate
2-(3-Methoxyphenoxy)ethanamine
Daltroban
6-FLUORO-2-METHYL-4-CHROMANONE
(R)-3-Quinuclidinol hydrochloride
2-Chloro-4-(2-furyl)pyriMidine
4-(ChloroMethyl)benzoyl chloride
Dibutyl Vinylboronate (stabilized with Phenothiazine)
4-Undecanol
TriphenylMethyl chloride, 98%
RNase A, Lyophilized Powder, High Purity
Vinyl Methacrylate (stabilized with MEHQ)
2-benzyloxy-1-ethanol
4'-(4-bromophenyl)acetophenone
Methyl 2-hydroxy-1-naphthoate
4-chloro-7-fluoroquinoline
Dimethyl oxalate
2-Cyanobenzaldehyde
1,1-Bis(Diphenylphosphino)Ferrocene PalladiuM Dichloride
N-FMoc-L-alanine Monohydrate, 98%
2-Fluoro-5-Methylbenzonitrile, 99%
Isocytosine
AbaMectin
DANSYL-L-VALINE
Phosphazene base P1-t-Bu-tris(tetraMethylene)
MONOCAPRIN
2,2'-Biphenol
Pyridine-2,6-dicarboxylic acid, 97%
2-Amino-4-(trifluoromethyl)benzonitrile , 98%
4'-Methylbiphenyl-2-carboxylic acid
Phenazine-1-carboxylic acid
2-AMINO-4-HYDROXYQUINOLINE HYDRATE
4,4'-DDT
2-Amino-5-bromine-2'-chloro benzophenone
Chlorhexidine;
MonoMethyl adipate
N,N'-Bis(2-aMinobenzal)ethylenediaMine
H-Orn(Boc)-OMe HCl
3-Acetyl-2,4-diMethylpyrrole, 98%
4-ForMylphenoxyacetic acid, 99%
DL-3,4-DIHYDROXYMANDELIC ACID
4-Methyl-1-naphthoic Acid
4-aminonaphthalene-1,7-disulfonic acid
1-[(2S)-2-(benzyloxy)propanoyl]pyrrolidine
Methyl 3-(bromomethyl)thiophene-2-carboxylate
2-(2-bromoethyl)-1,3-dioxane
Cyclohexanecarboxaldehyde
2-(2-BroMoacetyl)thiophene
2-Methylpyridine-4-boronic acid
(S)-(+)-2-Phenylpropionic acid
1,1-Dimethylhydrazine Hydrochloride
2,6-Dichloropyridin-4-amine
2-aminothiophene-3-carbonitrile(SALTDATA: FREE)
5,6,7,8-Tetrahydro-1,8-Naphthyridin-2-butylamine
N-Propylurea
PERILLIC ACID
1-(TRIMETHYLSILYL)-1-PENTYNE
L-XYLOSE
4-AMinophenol
1-AcetyliMidazole
4-(3-Hydroxyphenyl)piperidine
Boc-lys(z)-osu
Ethyl iMidazo[2,1-b][1,3]thiazole-6-carboxylate, 97%
Dibutyl sulfide
Azobenzene-4,4'-dicarboxylic Acid
(N,N-Dimethylamino)triethylsilane
Cupric Tartrate
Naphthol AS-VL
1,2:5,6-di-O-isopropylidene D-mannitol
MTF ELISA Kit
α1AT ELISA Kit
REG-4 ELISA Kit
Human SPTβN4 ELISA Kit
CFB ELISA Kit
TLR-4 ELISA Kit
SOD ELISA Kit
CCSA-3 ELISA Kit
Human Angiopoietin 1,ANG-1 ELISA Kit
ANG4 ELISA Kit
Murashge – Tucker Medium
Aerobe- Anaerobic Agar Medium
(S)-Metolachlor
Glycine
5-Chloro-2-fluorobenzeneboronic acid, 97%
2-Fluoro-6-hydroxypyridine, 97%
2-benzyloxy-1-ethanol
Foodyellow No.4 aluminum lake
Polyurethane
3,3'-difluorobiphenyl
(R)-N-Boc-1-Naphthylalanine
1,1,1-TRIFLUORO-5,5-DIMETHYL-2,4-HEXANEDIONE
Diethyl AcetaMidoMalonate
1,8-Octanedithiol, 98%
(R)-(+)-N-(3-Pentyl)-1-phenylethylaMine hydrochloride, 99%
Tri(ethylene glycol) bis(2-ethylhexanoate), 90+%
Methyl 2-Methylbenzoate
POTASSIUM (4-METHOXYPHENYL)TRIFLUOROBORATE
1-METHYL-2-PHENYLETHYL ACETATE
PENTAFLUOROBENZYL BROMIDE
(S)-(+)-2-INDOLINEMETHANOL
(R,R)-N,N′-Bis(3,5-di-tert-butylsalicylidene)-1,2-cyclohexanediaMinochroMiuM(III) chloride
2-Decanone, 98%
3-(3-Iodo-phenyl)-3-oxo-propionic acid ethyl ester
N,N,N',N'-Tetramethyl-1,2-diaminopropane