FAO细胞系—小鼠肝癌细胞系哪家提供 货号:GD-C16320142
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞系—T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞系—可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞系—并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-BroMo-2-(Methylthio)pyriMidine, 97%
2-AcetaMido-2-deoxy-β-D-glucopyranose 1,3,4,6-tetraacetate
(XYL)2P(O)H
2-NITRO-5-PIPERIDINOPHENOL
6-ACRYLAMIDOHEXANOIC ACID
N-(Chlorocarbonyl)isocyanate
DiMethyl naphthalene-2,6-dicarboxylate, 99+%
4-Sulfonic calix[6]arene hydrate, 95%
2-Bromo-3-pyridinemethanol
2-oxo-2,3-dihydro-1H-benzimidazole-5-carbonitrile(SALTDATA: FREE)
Potassium sulfide, anhydrous, min. 95%
1-(Hydroxymethyl)cyclopropaneacetonitrile
Methyl 5-thiazolecarboxylate
D-Cyclopentylglycine
2,6-DibroMophenol
Methyl 4-trifluoroMethylbenzoylacetate
N,N-DiMethylthioforMaMide
N,N-Diethyl 3-broMobenzenesulfonaMide
trans-Anethole, 99%
PIPES SesquisodiuM Salt, Biological Buffer, High Purity
16-DOXYL-stearic Acid Free Radical
Dihydrokavain
2,2,6-trimethyl-4H-1,3-dioxin-4-one
disodium tartrate
2-(4-Chlorophenyl)ethylamine
HEPTANAL DIMETHYL ACETAL
Phenylpropyl aldehyde
IMidazo[1,2-a]PyriMidine
PropargyltriphenylphosphoniuM broMide, 97%
1-Methyl-3-n-octyliMidazoliuM chloride, 97%
N,N'-DiMethylpropyleneurea
Z-Thr-NH2
4-BroMo-7-chloro-8-Methyl-2-(trifluoroMethyl)quinoline
Pyrazolo[1,5-a]pyridine-7-carboxaldehyde, 97%
DL-METHIONINE METHYLSULFONIUM CHLORIDE
2,3-Difluoro-4-MethoxybenzylaMine, 97%
4-(2-Aminoethyl), N1-BOC protected 95%
N-Carbobenzoxy-S-phenyl-L-cysteine
2-Octanone ,99%
4'-Nitro[1,1'-biphenyl]-4-carbaldehyde
Dimethylolurea
FLUORESCEIN DIACETATE
3-(Methylsulfonyl)phenylboronic acid
Cinnamamide
5-BroMovanillin
2-AMino-6-Methylbenzothiazole
Pht-Val-OH
3-Chloro-2-chloroMethyl-1-propene, 96%
N-(4-BroMophenyl)MaleiMide, 98%
MANGANESE(II) ACETATE TETRAHYDRATE
Cyclohexane [for SpectrophotoMetry]
3-bromo-4-methoxybenzoic acid
3-(Cyclohexylmethoxy)pyrrolidine hydrochloride
Disperse Yellow 50
4-Amino-3,5-dichloroacetophenone
2,2-DIMETHYL-6,6,7,7,8,8,8-HEPTAFLUORO-3,5-OCTANEDIONE
7-HEXADECANONE
3-(DiethylaMino)-1,2-propanediol
1-(4-CHLOROPHENYL)-1-CYCLOPENTANECARBOXYLIC ACID
Cyclopropylzinc bromide, 0.5M solution in THF, AcroSeal
1-(2-Bromo-ethyl)-2-chloro-benzene
3-Bromo-5-(trifluoromethyl)benzaldehyde 97%
4,6-Dinitroresorcinol (wetted with ca. 20% Water, containing 5g on a dry weight basis)
7-Methoxy-6-(3-morpholin-4-ylpropoxy)quinazolin-4(3H)-one
4-Methylmetanilic acid
DIPHENYL SELENIDE
Triphenylformazan
2-Methyl-3-nitrobenzoic Acid
1,4-Bis(4-aMinophenoxy)benzene
Ethyl 2-(2,5-dichlorophenyl)-2,2-difluoroacetate
Acetylcholine chloride, 99%
N,2,3-TriMethyl-2-isopropylbutaMide
2-PropoxyethylaMine
Methyl Tetrolate
4-Hydroxy-2-butanone
3-Carbazolylpropyltriethoxysilane
4-Chloro-2-fluorobenzyl chloride
N(alpha)-Z-D-2,3-diaminopropionic acid
APAF1 ELISA Kit
β-Defensin ELISA Kit
Human true insulin,TI ELISA Kit
Human SFRP5 ELISA Kit
CNTF ELISA Kit
P450scc ELISA Kit
Chicken α-Glucosidase,a-Glu ELISA Kit
Human MAPKAPK3 ELISA Kit
AT2R ELISA Kit
FN ELISA Kit
Yeast Synthetic Drop-out Agar Medium
Glucose Peptone Medium
Isosorbide diMethyl ether, 98%
Copper(II) Perchlorate Hexahydrate
Ethyl 5-chloroindole-2-carboxylate, 98%
2-Fluoroisoniazide
Dihydrokavain
N,N',N'',N'''-Tetraacetylglycoluril
(1R)-(+)-Alpha-Pinene
2-Fluoro-4-methoxybenzaldehyde
(-)-Corey lactone diol
Magnesium chloride hexahydrate
1-Butanethiol
Phenylsuccinic anhydride, 99%
N^a-Benzyloxycarbonyl-L-2,3-diaMinopropionic acid, 98%
2-Chloro-6-(trifluoroMethyl)benzoyl chloride, 97%
3-Chloro-1-propanol
Tris(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)tin azide
(4-METHYLBENZYL)TRIPHENYLPHOSPHONIUM BROMIDE
CIS-1,2-BIS(DIPHENYLPHOSPHINO)ETHYLENE
1,3-DibroMo-5,5-diMethylhydantoin
(1S,2S)-2-BENZYLOXYCYCLOHEXYL ISOCYANATE
1,2-Dichlorobenzene
Dithiobiuret
1-Bicyclo[2.2.1]hept-5-en-2-ylmethanamine