Kert-3细胞系—小鼠肾癌细胞系谁家更好 货号:GD-C16220538
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-BroMo-3-(trifluoroMethoxy)salicylaldehyde, 99%
(R)-4-Chloro-3-hydroxybutyric Acid Ethyl Ester
ButyraMide
2-Fluoro-3-Methylpyridine-5-boronic acid, pinacol ester
2-PIPERIDYLMETHYLAMINE
(+)-Menthyloxyacetic acid
4,5-Dimethoxy-isatoic anhydride
2-Fluoro-3-methoxybenzaldehyde
Curcumin (Synthetic)
N-(2-fluorobenzyl)-2-methyl-2-propanamine(SALTDATA: HCl)
(R)-N-Phthaloyl-2-aminobut-3-en-1-ol, 95%, (95% ee)
N-(Isoxazol-5-yl)sulphanilamide
Etodolac methyl ester
Cyanuric chloride
1-Fluoro-2-iodobenzene
1-Tetradecanol
2,5-Difluoropyridine
N-Propyl 3-broMo-5-nitrobenzaMide
BroMotriethylsilane, 95%
TriMebutine Maleate Salt, JP
2,6-Difluorobenzyl Chloride
1-(3-(5-nitro-2-furyl)-1-(2-(5-nitro-2-furyl)vinyl)allyliden...
phenylethynylmagnesium bromide
Solvent blue 5 (C.I. 42595:1)
sodium cinnamate
4-Chlorobenzyl cyanide
Estradiol valerate
6-(1H)INDAZOLE CARBOXYLIC ACID METHYL ESTER
4-Methoxybenzaldehyde, 98%
2-Methylpyridine-4-carboxylic acid, 96+%
IodoforM
1-Chlorooctane
3-(2-Ethoxy-2-oxoethoxy)phenylboronic acid
N-Methyl 5-chloro-2-Methylaniline, HCl
2,3,4,6-Tetra-O-acetyl-alpha-D-galactopyranosyl 2,2,2-TrichloroacetiMidate , 93%
2-Butylthiopyridine-5-boronic acid
4-(4H-1,2,4-triazol-4-yl)phenol(SALTDATA: FREE)
5-Acetylamido-2-chloroaniline, 98%
Vinblastine sulfate, 96.0-102.0%
1-(4-Chlorophenyl)-2-piperazinone
Fmoc-Thr(HPO3Bzl)-OH
2-Bromoethyl acetate
HYDROGEN SELENIDE
Indium
alpha-BroMo-2,3,4,5,6-pentafluorotoluene
2-Cyclohexen-1-ol
2,5-DiMethylphenylacetonitrile
4-Chloro-1h-pyrrolo[2,3-b]pyridine-5-carbaldehyde
4-Iodo-5-Methyl-isoxazole, 97%
Xylenol Orange, Water Soluble
3-AMino-3-phenyl-1-propanol
1,12-Dodecanedicarboxylic acid
5-hydroxytoluene-2-sulphonic acid
1,3-Butanedione, 1-(4-ethylphenyl)-4,4,4-trifluoro-
1-Iodo-3-methyl-butane
GALLIUM(III) CHLORIDE
2-(Methoxycarbonyl)-3-(trifluoroMethyl)phenylboronic acid
2-Methylbenzothiazole
N-Isobutyl 2-broMo-5-fluorobenzaMide
Diphenylacetaldehyde, 97%
1-Phenylcyclopropanemethylamine, 97%
3-(N-Acetyl-N-methylamino)pyrrolidine
2,3-Dihydro-2-spiro-4'-[8'-aminonaphthalen-1'(4'H)-one]perimidine (contains o-form)
2-Amino-6-methyl-5-nitropyridine
Boc-DL-alanine
6-BROMO-1,3-BENZOTHIAZOLE
p-Nitrobenzamide
3-Nitrostyrene
1H-Cyclopenta[l]phenanthrene
5-Pyrrolidinopyridine-3-boronic acid
Inosine 5'-Monophosphate, disodiuM salt hydrate, 97%
PropargyloxytriMethylsilane, 97%
1-Benzyl N-(tert-Butoxycarbonyl)-L-aspartate
(R)-(-)-1-aminopropan-2-ol
3,4-Dichlorobenzylamine
3-BROMO-5-FLUOROBENZALDEHYDE
Trifluoromethyltriethylsilane
EPI ELISA Kit
KLβ ELISA Kit
Human EuglobulinLysis,EL ELISA Kit
PTN ELISA Kit
INS ELISA Kit
IFI16/p16 ELISA Kit
Chicken cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
PKCz ELISA Kit
Human platelet basic protein,PBP ELISA Kit
CEP110 ELISA Kit
Lactose Broth
PH7.2 phosphate buffer (Medicine)
Vinyl(chloroMethyl)diMethylsilane, 97%
Phenazine
4'-n-Octyloxyacetophenone
2-Iodobenzene-1-Sulfonyl Chloride
1-(3-(5-nitro-2-furyl)-1-(2-(5-nitro-2-furyl)vinyl)allyliden...
4-chloro-3-ethylphenol
AMMONIUM-D4 BROMIDE
isopropyl nitrite
5,6-Dimethoxy-1-indanone
4-Chloro-3-nitroanisole
Boc-Sar-OH
(-)-1,2-Bis[(2S,5S)-2,5-diethyl-1-phospholanyl]ethane, 97+%
1,8-DiaMinonaphthalene, 97%
3,3-Diphenyl-L-alanine
N-Boc-4-Hydroxypiperidine
2-(BROMOMETHYL)-6-(HYDROXYMETHYL)PYRIDINE
(S)-5-HydroxyMethyldihydrofuran-2-one
2'-Methoxyacetophenone
3-Hydroxy-4-Methoxybiphenyl-3-carboxylic acid
2,6-Dichlorophenol
5-Bromoquinoline ,97%
2,3-Dihydro-1-benzofuran-5-ylboronic acid , May contain varying amounts of anhydride, 95%
Dimethyl carbonate, 99%