NCI-H1975[H-1975,H1975]细胞系—人肺腺癌细胞系谁家更好 货号:GD-C16220433
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Nitrobenzaldehyde, 99%
BenzyltriMethylaMMoniuM chloride
Phenethyl phenylacetate
7-METHYL-1,4-BENZOTHIAZIN-3-ONE
D-HoMophenylalanine ethyl ester hydrochloride
1,2:3,4:5,6-tri-O-isopropylidene-D-Mannitol
GiMeracil
(6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-3-ylmethyl)amine(SALTDATA: 2.3CF3COOH 0.3H2O)
Potassium (4-formylphenyl)trifluoroborate
4-Chloro-4'-hydroxybenzophenone
(3S,6S)-3-Benzyl-1,4-diazabicyclo[4.3.0]nonane
BOC-4-OXO-PRO-OME
1,2-BENZANTHRACENE
1,4-Dibenzoylbutane
DL-Tartaric acid
6-Chloroindole
Meso-1,2-DiphenylethylenediaMine
Methyl 3-broMo-5-Methoxybenzoate
Methyl 3-isocyanatobenzoate, 97%
Sulfanilic Acid, Anhydrous, Reagent, ACS
N-(3-Hydroxypropyl)ethylenediaMine
4-Isopropylacetophenone
4-Amidinopyridinium chloride
Tetracycline Hydrochloride
2,6-Pyridinedicarboxylic acid
TERBIUM(III) CARBONATE HYDRATE
Ethyl 4,4,4-trifluoroacetoacetate
SaMariuM(Iii) Chloride Hexahydrate
1-Methyl-2-(tri-n-butylstannyl)indole, 96%
Crystal Violet
trichloroacetic acid
Zincon
PotassiuM (4-Methoxycarbonylphenyl)trifluoroborate
O,O'-BIS(TRIMETHYLSILYL)-5-FLUOROURACIL
4-ForMyl-3,5-diMethylphenylboronic acid
2-(2-ChloroacetaMido)-4-thiazoleacetic acid
2-Fluoro-5-(trifluoromethyl)benzylamine
1-[3-(4-Methylpiperazin-1-yl)phenyl]methanamine , 97%
2-Naphthyl Acetate
(4'-Nitro[1,1'-biphenyl]-4-yl)methanol
SEC-BUTYL ISOTHIOCYANATE
2-Phenylbenzimidazole-5-sulfonic acid
FEMA 2871
Isobutyric anhydride
5-BroMo-2-chloropyridine
2,4-Dinitroanisole
Nitrosobenzene
2-Hydrazinyl-6-Methylpyridine
1,2-Dihydronaphthalene, 96%
Potassium hydroxide
2,3-Pyrazinedicarboxylic Acid
4-(tert-butoxycarbonyl)benzoic acid
1-Bromo-4-butylbenzene
poncirin
1-benzyl-3-pyrroline
YTTERBIUM FLUORIDE OXIDE
HEXAMETHONIUM CHLORIDE
4-Fluoro-3-(Morpholine-4-carbonyl)phenylboronic acid
Glyoxal Mono-diMethylhydrazone
1,3-Bis(chloromethyl)tetramethyldisiloxane
5- p -Tolyl-2 H -pyrazole-3-carboxylic acid
Ponceau SX
Methyl (S)-(-)-2-Isocyanato-4-methylvalerate
1-Boc-3-(Amino)azetidine
Bis(trimethylsilyl)trifluoroacetamide
Methyl 3-bromo-4-methylbenzoate
Sulfapyridine Melting Point Standard (ApproxiMately 191 degrees)
Ethyl 4-Piperidinecarboxylate
1-Butoxy-2-propanol
2'-Methoxy-6'-Methylbiphenyl-3-carboxylic acid
6-BroMohexanenitrile, 95%
2-Methyl-6-quinolinecarbaldehyde, 97%
1H,1H,10H,10H-Hexadecafluoro-1,10-decanediol
N,N'-Di-n-octyl-3,4,9,10-perylenetetracarboxylic DiiMide
Furoin
Boc-D-Arg(Mtr)-OH
4-bromo-1-methyl-1H-pyrazole-5-carboxylic acid
N-(3-fluorobenzyl)butan-1-amine
AREG ELISA Kit
Mouse RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2) ELISA Kit
Human PCDH8 ELISA Kit
Human DPP6 (Dipeptidyl Peptidase VI) ELISA Kit
CD3 ELISA Kit
Aβ1-42 ELISA Kit
PLCγ1 ELISA Kit
Human ELA4 ELISA Kit
Human SAA ELISA Kit
INHβB ELISA Kit
MIO Medium
Modified Y Medium
Copper(II) 2,4-pentanedionate, 98%
Phosphorus(III) fluoride, 99%
(R)-(+)-5,5',6,6',7,7',8,8'-OCTAHYDRO-1,1'-BI-2-NAPHTHOL
2-Fluoro-3-iodopyridine, 97%
4-Isopropylacetophenone
2,5-Dimethylphenylacetic acid
Transferrin
Imidazolidine
2-Bromo-2-nitro-1,3-propanediol
1-Bromo-5-chloropentane
ethyl 1H-indazole-4-carboxylate
HexacarbonylMolybdenuM, 98%
Norbornene, 99%
4-Fluorothioanisole
4-HydroxythiobenzaMide
Isobutyl 1,2-dihydro-2-isobutoxy-1-quinoline-carboxylate
4-(2-MethoxyethylcarbaMoyl)phenylboronic acid, pinacol ester
2-(TRIFLUOROMETHYL)PHENETHYL ALCOHOL
Halosulfuron-Methyl
4-Phenyl-1-buten-4-ol, 97%
N-Boc-2-maleimidoethylamine
5-Hydroxy-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carbaldehyde
Copper(I) bromide-dimethyl sulfide complex, 99%