Ealy926细胞系—人肠系膜下腔动脉血管内皮细胞系谁家更好 货号:GD-C16219234
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-BroMo-3-hydroxyoxindole, 96%
2-AMinopyriMidine
Diethyl 2-((3-chloro-4-MethylphenylaMino)Methylene)Malonate
BENZOYL FLUORIDE
Diallyl Maleate
4-BENZYL-3-THIOSEMICARBAZIDE
STRONTIUM CYCLOHEXANEBUTYRATE
3-Iodoaniline, 98%
Tris(2,2,6,6-tetramethyl-3,5-heptanedionato)gallium(III), 99% (99.999%-Ga) [Ga(TMHD)3] PURATREM
1-Chlorooctadecane
4,5-Bis(benzoylthio)-1,3-dithiole-2-thione
4-BROMOPHENYL ISOTHIOCYANATE
Diallyl 2,2'-oxydiethyl dicarbonate
Methyl isovalerate
3-(TriMethoxysilyl)propyl acrylate
4-(Pyrrolidin-1-ylMethyl)benzylaMine
2,9-Diphenyl-1,10-phenanthroline
N-Methyl 3-broMo-4-fluorobenzaMide
YttriuM(III) chloride hexahydrate, 99.999%, (trace Metal basis)
Spironolactone, USP
N,N,N',N'-TetraMethyl-1,4-diaMinobutane
D-Valine
4-Nitrobenzenesulfonamide
5-Iodocytidine
3-fluorobenzyl alcohol
2,5-Dichlorophenylhydrazine hydrochloride
4-(Trifluoromethyl)benzylamine
2-Chloro-3-nitropyridine
5-Methylthiophene-2-boronic acid, 98%
Silicon(IV) acetate
Bis(acetylacetonato)dioxoMolybdenuM(VI)
L-GlutaMic acid γ-benzyl ester
4-BROMO-4'-N-PENTYLBIPHENYL
O-TOLYL BENZOATE
1,5-NaphthalenediaMine
Methyl 3-aMino-4-Methoxybenzoate
Orotic acid ,99%
2,4-Difluorothiobenzamide
3-Indolyl-β-D-glucuronide cyclohexylammonium salt ,97%
4-(Methylamino)-3-nitrobenzoic acid
Mordant Orange 1
N,N-DIMETHYLINDOLINE-5-SULFONAMIDE
4-Bromo-2-fluorobenzotrifluoride
3-Ethyl-3-pentanol;Triethylcarbinol
2-(4-Methoxyphenoxy)ethylaMine
ert-Butyl phenyl carbonate
N-Octadecyl-4-stilbazole BroMide
3-Chloro-1-(2-fluorophenyl)propan-1-one
(2-Hydroxyethyl)triphenylphosphoniuM chloride, 98%
3-Indolebutyric acid
5-(Ethylthio)-1H-tetrazole
Potassium polycalcium
3-BROMO-2-METHYLBENZONITRILE
Ethyl 3-benzoylacrylate
9-Fluoro-11,17,21-trihydroxypregna-1,4-diene-3,20-dione 21-acetate
4,4'-DIBROMOOCTAFLUOROBIPHENYL
5-Acetyl-2-aMino-4-MethylpyriMidine, 97%
Methyl 2-broMo-5-Methylbenzoate
Hexyl salicylate
N-(3-Hydroxy-4-propylphenyl)acetamide
2-Phenylethylthiourea
Gal[236Bn]beta(1-4)Glc[236Bn]-beta-MP
3,5-(Difluorophenyl)methanesulfonyl chloride
Ethyl 2,6-dichloro-5-fluoro-pyridine-3-acetoacetate
Butyl levulinate
2-Hydroxy-6-chloroquinoxaline
Mesotrione
Cholesteryl chloroforMate
1-BroMo-3-chloro-2-fluorobenzene
4-(CyclopropylMethoxy)phenylboronic acid
cis-5-Norbornene-endo-2,3-dicarboxylic acid, 99%
trans-(+)-ChrysantheMic acid, 99+%
Decahydro-2-naphthyl ForMate (Mixture of isoMers)
9-AMinocaMptothecin
3-Chlorobenzophenone
sitagliptin phosphate
hydroxytacrine maleate
Iron (III) tris(4-methylbenzenesulfonate)
ACP ELISA Kit
S100A8 ELISA Kit
Human TPM4 ELISA Kit
Human UBN2 ELISA Kit
Chicken IFN-β (Interferon Beta)ELISA Kit
β2AR ELISA Kit
FGF2 ELISA Kit
CCK ELISA Kit
Human plasma anticoagulant protein C,PC ELISA Kit
PANDER ELISA Kit
PAC Slant Medium
Fluid soybean-casein Digest Broth
Acetyl bromide
CeriuM(IV) fluoride hydrate
5,6,7-Trihydroxyflavone, 97%
2-Fluoro-4-iodopyridine
D-Valine
4,6-dimethoxy-2-methylpyrimidine
2-(1-cyano-1-methylethyl)azocarboxamide
p-allylphenol
1,3-BENZOTHIAZOL-5-AMINE
sec-Butyl disulfide
3-BroMo-2-Chlorobenzonitrile
RhodiuM(IV) oxide, Rh 75.2-77.4%
2-Fluoronicotinic acid, 97%
N,N-DiMethylindoline-5-sulfonaMide, 98%
DiphenaMid Standard
ISOBUTYLMAGNESIUM BROMIDE
MANGANESE PENTACARBONYL BROMIDE
N-ETHYLPROPIONAMIDE
(S)-(-)-1-Methyl-2-(1-naphthylaMinoMethyl)pyrrolidine , 97%
ALPHA,ALPHA'-DIANILINO-P-XYLENE
Di-tert-butyl Disulfide
2-Chloroethylphosphonic acid, tech., 90%
3-Methyl-2-(trifluoromethyl)pyridine