CBRH-7919细胞系—大鼠肝癌细胞系谁家更好 货号:GD-C16219106
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Chloroacetaldehyde diMethyl acetal, 98%
2,5-Dinitrophenol
1-Ethoxy-2-propanol
BUTYL LAURATE
3-BroMo-5-pyrrolidinopyridine
Gly-Leu-Tyr
N-BENZYLIDENEMETHYLAMINE
2-Trifluoromethylbenzylsulfonyl chloride
4-Nitrophenyloxamic acid
4-Propylcyclohexanecarboxylic Acid (cis- and trans- mixture)
Sulfisomidine
BOC-L-Asparagine
Guanidine hydrochloride
D(+)-Phenylalaninol
2-Fluoro-5-nitroaniline
2,4-Dihydroxypyridine
2',3'-Didehydro-3'-deoxythyMidine
Usnic Acid
Tri(2-Methylphenyl)phosphine, 97%
Ribavirin, USP
N-(tert-Butoxycarbonyl)-2-nitrobenzenesulfonaMide
Cinnamamide
4-Methoxyphenylboronic acid
3-(trifluoromethylthio)benzoic acid
Nalidixic acid
(Carbomethoxymethyl)triphenylphosphonium bromide
Prednisolone succinate
6-Methoxynicotinic Acid
BroMotriphenylethylene, 98+%
3-Methyl-2-buten-1-ol, 98+%
Methyl nonanoate
TributylMethylaMMoniuM chloride
1,4,10,13-TETRAOXA-7,16-DIAZACYCLOOCTADECANE
PHOSPHORIC ACID TRI-N-AMYL ESTER
4-Chloro-2-Methoxypyridine
N-TERT-BUTYLMALEIMIDE
Pyrimidine-5-boronic acid, pinacol ester, 97%
Biurea, 99%
Diacetato{(S)-(-)-5,5'-bis[di(3,5-xylyl)phosphino]-4,4'-bi-1,3-benzodioxole}ruthenium(II) Ru(OAc)2[(S)-dm-segphos]
4-(2-Aminoethyl)pyridine hydrochloride
2-Fluoro-4-methylbenzoic acid
(S)-(-)-2-AMINO-1,1-DIPHENYL-1-PROPANOL
SILVER CYCLOHEXANEBUTYRATE
2,4-Dinitrophenylhydrazine
2-Benzo[b]furan-3-ylethanol
Etridiazole
Neu5Gc alpha(2-6)Gal beta MP Glycoside
5-(3-Chlorophenyl)isoxazole
2-(3,5-Difluorophenyl)ethanoyl chloride, 97%
1-N-HEXYLTHEOBROMINE
2-BroMobenzenesulfonyl Chloride
2-Cyclohexen-1-ol,1-methyl-4-(1-methylethenyl)-, (1S,4R)-
5-Methylcyclocytidine hydrochlorine
2H-Tetrazole, 2-methyl-
vanadium tetrachloride
ALPHA-CHLORO-3-NITROACETANILIDE
1-BroMo-2-Methyl-4-trifluoroMethoxybenzene
N-(4-Acetylphenyl)thiourea, 98%
TRIS(2-METHOXYPHENYL)BISMUTHINE
1-(2,4-Dimethoxyphenyl)-2-thiourea
Benzo[b]thiophene-2-carbohydrazide, 97%
6-Bromo-2-hydroxyquinoline-4-carboxylic acid
Ethyl 4-cyano-5-(methylthio)thiophene-2-carboxylate
L-Norvaline ethyl ester hydrochloride
2-Methyinaphtho[1,2-d]thiazole
2-Furanacrolein
;
1,3,5-TriMethyl-1H-pyrazole-4-carbaldehyde
1-Acetyl-1,2,3,4-tetrahydroquinoline
4,5,6,7-Tetrahydrothieno[2,3-c]pyridine, HCl
4'-AMinoacetophenone, 99%
2-(1-Naphthyl)ethanoyl chloride, 95%
Analgin
6,6'-DiMethyl-2,2'-bipyridyl
Octanoic acid
1-BROMO-4-CHLORO-2-FLUORO-5-METHOXY-BENZENE
Phenol, isopropylated, phosphate (3:1)
2-Bromo-L-phenylalanine
VD ELISA Kit
A1-MG ELISA Kit
Human REG3γ ELISA Kit
Human TNK2 ELISA Kit
O2 ELISA Kit
TLR-2 ELISA Kit
C4 ELISA Kit
Human colon cancer-specific antigen-2,CCSA-2 ELISA Kit
Human VCAM-1/CD106 ELISA Kit
VEGF.D ELISA Kit
NS medium
Wilkins-Chalgren Anaerobe Broth
BariuM tungsten isopropoxide, 10% w/v in isopropanol
SODIUM GLYCINATE
5-Chloro-2-fluoronitrobenzene
2-BroMo-6-Fluorobenzonitrile
Cinnamamide
Sulphur Green 14
Cytochrome C
1-(bromomethyl)-2-(trifluoromethyl)benzene
Methylthiouracil
L-(+)-Prolinol
N-Allylthiourea, 98%
Ethyl 2,2,3,3,3-pentafluoropropyl carbonate, 97%
Thionyl chloride, 1M soln. in dichloroMethane
Nylon 6
2-Hydroxy-5-trifluoroMethylpyridine
INDIUM PHOSPHIDE
(R)-1-Phenyl-2-propyn-1-ol
2-(2-HYDROXYETHYLAMINO)-5-NITROBENZONITRILE
1-BroMo-4-(2-broMoethyl)benzene
3-Hydroxy-2-naphthanilide
Copper(II) carbonate, basic
6-Bromo-1H-indazole, N1-BOC protected
3',6'-Bis(diethylamino)-2-(4-nitrophenyl)spiro[isoindole-1,9'-xanthene]-3-one