CHL-1细胞系—ZG仓鼠肺细胞系哪家专业 货号:GD-C16120510
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
N^a-Acetyl-D-arginine dihydrate, 99%
Benzyl 2-broMoethyl ether
FMoc-L-hoMophenylalanine
2,2-Dichloro-3,3-bipyridine, 94%
Carbaryl
3-Picoline
2-Amino-4-methyl-thiazole-5-carboxylic acid methyl ester
2,2-Difluoroethyl trifluoromethanesulfonate
2-amino-4,6-dimethylnicotinonitrile(SALTDATA: FREE)
4-(Trifluoromethoxy)benzaldoxime
2-Bromo-6-methoxybenzoic acid
5-NITRO-2-FURONITRILE
3-CHLORO-5-(TRIFLUOROMETHYL)PYRIDINE-2-THIOL
Chitin
4-Cyano-4'-heptylbiphenyl
N'-Hydroxy-2-MethylpropaniMidaMide
trans-2,6-DifluorocinnaMic Acid
Methyl 3,5-dibroMopyrazine-2-carboxylate
1-Butoxy-4-eth-1-ynylbenzene, 97%
Tizanidine Hydrochloride, USP
2-BroMo-4-chloro-6-fluoroaniline
3,5-Dimethyl-1-adamantanol
2,4,6-Trichloro-5-fluoropyrimidine
Montmorillonite
pentacosanoic acid
4-Methylbenzenesulfonhydrazide
N-BENZOYLADENOSINE
1-Methyl-1H-Pyrazole-4-Carbaldehyde
DiMethylaMine, 2M in THF
1-(1-NaphthylMethyl), 97%
2-AMino-6-Methyl-5-nitropyridine
TetraethylaMMoniuM tetrafluoroborate
4-NITROCHALCONE
N-Methylpyrrole-2-carboxaldehyde
BENZYL 4-BROMOTETRAHYDRO-1(2H)-PYRIDINECARBOXYLATE
Dibenzyl dicarbonate
N-methyl-N-(pyridin-2-ylmethyl)glycine(SALTDATA: 2HCl H2O)
Benzoic anhydride ,98%
3,3'-Thiodipropionic acid, 99%
Isopropyl Methyl Sulfone
2,4-Dichlorophenylhydrazine hydrochloride
N,N-DIETHYL-2,2,2-TRIFLUOROACETAMIDE
4-Chloro-3,5-dinitrobenzoic acid
3,5-Difluorobenzyl bromide
1,2-Propanediol
1-Iodo-2-(trifluoroMethoxy)benzene
3-EthylaMino-p-cresol
4-ForMyltetrahydropyran
Ethyl 3-ethoxy-2-(2,2,2-trifluoroacetyl)acrylate, Tech.
4-BROMOCHLOROBENZENE
1,8-DibroMohexadecafluorooctane
(E,E)-6,10,14-trimethylpentadeca-5,9,13-trien-2-one
tributylvinylstannane
N-(2-((2,6-DIMETHYLPHENYL)AMINO)-2-OXOETHYL)-N,N-DIETHYLBENZENEMETHANAMINIUM CHLORIDE
1,4-dichloroanthraquinone
(S)-2-(2-NAPHTHYLMETHYL)SUCCINIC ACID-1-METHYL ESTER
tert-Butyl phenyl ether, 98%
Phenol Red
BENZALBARBITURIC ACID
SILICON TETRAACETATE
8-Chloro-2-methylquinazolin-4(3H)-one
Boc-o-Methyl-D-serine
m-Toluidine Hydrochloride
Tricyclohexylchlorotin
4-CHLORO-3-NITROBENZONITRILE
2,3-O-ISOPROPYLIDENE-D-ERYTHRONOLACTONE
Androsta-1,4-diene-3,17-dione
3-FLUORO-4-METHOXYCARBONYLPHENYLBORONIC ACID
1-Dodecanethiol
5,6-Dihydro-2H-pyran-2-one
2-(3-BroMophenyl)-5-ethyl-1,3,4-oxadiazole
SodiuM azide, 99+%, for biocheMistry
LithiuM Carbonate, Powder, Reagent, ACS
DiMethocaine
3,5-Dinitrotrifluorotoluene
2-Chloro-3-nitrobenzoic acid
1,2-diiodobenzene
4-styrylpyridine
GH ELISA Kit
ND1 ELISA Kit
Human F-TESTO ELISA Kit
Human OTOP2 ELISA Kit
NT-proBNP ELISA Kit
β-TG ELISA Kit
Chicken growth hormone,GH ELISA Kit
PKC ELISA Kit
Human THBS2 ELISA Kit
IG-α2β1 ELISA Kit
VRE Agar Base
Todd-Hewitt Agar
Ethephon
Nitrofurantoin
5-(2-Furyl)isoxazole-3-carboxylic acid, 97%
2-Iodophenylacetic Acid
3,5-Dimethyl-1-adamantanol
2-Cyanopyrimidine
ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine
amyl butyrate
DIALLYL MALEATE
SodiuM Thiophenoxide
4-Methylbenzyl broMide, 98%
2-n-Hexyldecanoic acid, 97%
4-n-Pentylphenyl 4-n-pentylbenzoate, 97%
5-Methoxy-1H-pyrrolo[2,3-b]pyridine
Diethyl propylMalonate
4,4'-AZODIANILINE
(S)-N-Benzyl-1-(1-naphthyl)ethylaMine hydrochloride
4-(TrifluoroMethylthio)aniline
2,5-DIHYDROXY-1,4-BENZOQUINONE
Tris(trimethylsilyl)amine, 99%
BOC-D-Methionine, 98%
Ethyl 2-(2-formylphenoxy)acetate
1-(3,4-Dimethoxyphenyl)-2-(2-methoxyphenoxy)-propane-1,3-diol