Lec1细胞系—仓鼠卵巢细胞系哪家专业 货号:GD-C16119230
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,6-DibroMo-4-nitrophenol, 98%
Ethyl diazoacetate
N-ACETYLGUANIDINE
4-BroMopiperidine hydrobroMide
trans-4-Methylcyclohexanecarboxylic acid
4-Benzyloxy-3-Methylphenylboronic acid
N-(HydroxyMethyl)phthaliMide
3-Amino-5-bromobenzoic acid 98%
2-(Pyridin-3-yl)benzaldehyde 97%
Methyl Succinamate
3-Aminopyridazine trihydrochloride
BROMOTRIFLUOROETHYLENE
2,3-Dimethyl-5-ethylpyrazine
2,6-Difluoroanisole
Methyl 2-BroMopropionate
Diphenylcyclopropenone
Diethylene Glycol 2-BroMoethyl Methyl Ether
3-(BOC-AMino)benzaldehyde
YtterbiuM(III) triflate hydrate, 99.9%, (trace Metal basis)
Soybean Oil, USP
1-Phenyl-1-hexanol
Triphenyltin Chloride
(DL)-(1-Aminoethyl)phosphonic acid
N-Carbobenzyloxy-L-serine
4-nitrobenzenediazonium tetrafluoroborate
Sodium octanoate
PEROXIDASE
2-Quinoxalinecarboxylic Acid
1,4-Benzenediacrylic acid, 98%
Ethyl 2-(ethoxycarbonylMethoxy)benzoate, 97%
8-BroMoadenosine
6-AMinobenzothiazole
1-(Benzyloxy)-4-broMo-2-Methoxybenzene
Oxalic acid
2-AMino-4-Methoxy-6-Methyl-1,3,5-triazine
5-Butoxy-2-chlorophenylboronic acid
N-Piperidin-4-yl-benzamide
3-Nitrophenol
7-Methoxycoumarin-3-Carboxylic acid
N-(tert-Butoxycarbonyl)-D-glutamic Acid
Pralmorelin
LITHIUM CITRATE TETRAHYDRATE
4-FLUORO-2-(TRIFLUOROMETHYL)PHENYLACETONITRILE
3-Phenylpyridine;β-Pyridyl benzene
4-Chloro-1,8-naphthalic anhydride
2-Fluorothioanisole
Methyl 2-Octynoate
Methyl 2-aMino-4-(trifluoroMethyl)benzoate
1-Methyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-indole, 97%
TWEEN(R) 65
Methyl CarbaMate
4-hydroxy-3-methylphenyl hexopyranoside
4,4'-(1,3-Propanediyl)dioxydianiline
7-Bromo-6-chloro-4-quinazolinone
croton oil
1-Iodohexane
DIETHYLMETHYLVINYLSILANE
1-METHYLHYDANTOIN
1-(3,4-Dichlorophenyl)biguanide hydrochloride
2',3'-Dihydroxy-4'-methoxyacetophenone Hydrate
Heptaethylene Glycol
(S,S)-(-)-2,2'-Bis[(R)-(N,N-dimethylamino)(phenyl)methyl]-1,1'-bis(di(3,5-dimethylphenyl)phosphino)ferrocene, min. 97%
5-fluoro-1-methyl-1H-indole-3-carbaldehyde(SALTDATA: FREE)
ETHYL ISONICOTINOYLACETATE
3-CHLORO-THIOBENZAMIDE
Mordant Black 9
Dimethyl acetylenedicarboxylate
3,5-Di-tert-butylsalicylic acid
1-BroMo-3,5-bis(tert-butylthio)benzene
8-Fluoro-4-hydroxyquinoline-2-carboxylic acid
CeriuM(IV) sulfate tetrahydrate, 99+%
3-Iodo-1H-pyrazole, 97%
DodecyldiMethyl(3-sulfopropyl)aMMoniuM Hydroxide Inner Salt [for BiocheMical Research]
3-Heptenoic Acid
3-Hexyne
Rosuvastatin intermediate
2-Pyridinamine, 5-fluoro-6-methyl-
6-bromo-5-methylpyrazin-2-amine
MPO ELISA Kit
S100A9 ELISA Kit
Human TMOD4 ELISA Kit
Human UBQLN4 ELISA Kit
IFNβ/IFNB ELISA Kit
α-TG ELISA Kit
Chicken basic fibroblast growth factor,bFGF ELISA Kit
Human BLVRB ELISA Kit
Human CETP ELISA Kit
GC ELISA Kit
42℃ Growth Medium
Columbia CNA Agar
Acetoxyacetyl chloride, 97%
TantaluM(V) fluoride, 99.9% (Metals basis)
5,6-Dichloronicotinic acid, 98+%
2-Fluoro-4-MethylbenzylaMine, 97%
Triphenyltin Chloride
H-Lys(Boc)-OtBu . HCl
White carbon black
Benzyl Chloroformate
8-Hydroxyquinoline-N-oxide
(R)-(-)-1-BENZYLOXY-3-(P-TOSYLOXY)-2-PROPANOL
2-Mercaptobenzoxazole
Iron nickel oxide, tech.
2,2-DiMethyl-3-pentanol, 97%
1,2-Dichloro-4-nitrobenzene, 99%
γ-Glu-ε-Lys
DisodiuM beta-glycerophosphate pentahydrate
5-CHLORO-2-ADAMANTANONE
2-(METHOXYCARBONYL)PHENYL ISOCYANATE
3-BroMo-2-hydroxybenzonitrile
SodiuM 2-(7-(3,3-diMethyl-1-(4-sulfonatobutyl)benz(e)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-diMethyl-1-(4-sulfonatobutyl)benz[e]indoliniuM
1-(4-Chlorophenyl)-3-phenyl-2-thiourea
1,2-Bis((2S,5S)-2,5-diisopropylphospholano)benzene(cyclooctadiene)rhodium(I) tetrafluoroborate, 97%
α-D-Glucopyranose pentabenzoate ,98%