Reh细胞系*人急性淋巴细胞系专业性培养 货号:GD-C15719112
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系*其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-BroMothiophene-2-carboxylic acid hydrazide, 98%
1,1′-Bis(diisopropylphosphino)ferrocene
Poly(4-vinylpyridine-co-ethylvinylbenzene), cross-linked
4-AMino-2-diMethylaMino-6-hydroxypyriMidine HeMihydrate, 98%
N-Benzyl 2-broMo-4-nitroaniline
CeriuM(III) nitrate hexahydrate
4-Methyl-3-nitropyridine
2-Anthracenecarboxylic Acid
Phytantriol (mixture of isomers)
Sodium orthovanadate, 99%
Chloromethyldimethylsilane
4'-CHLOROACETANILIDE
Arbutin
Glucose pentaacetate
2,1,3-Benzothiadiazole-4-carboxylic acid
2-BroMobenzylsulfonyl chloride
1,3-Dichloro-2-iodobenzene
Undecylcyclohexane
TriphenylMethane, 99+%
Risperidone, USP
TriMethylchlorogerMane
1,5-diphenyl-3-thiocarbonohydrazide
3'-fluorobiphenyl-4-amine
2-Methoxynaphthoic acid
6-Fluoro-4-hydroxyquinoline
1H,1H,2H,2H-Heptadecafluorodecyl acrylate
SodiuM Nitrite
2,2,4,4-TetraMethylpentane, 98%
4-BroMobenzylaMine hydrochloride, 98%
Methyl 3-oxo-3,4-dihydro-2H-1,4-benzothiazine-2-acetate, 98%
4-Nitrophenyl disulfide
N-PhenylbenziMidoyl chloride
2,5-Difluoroaniline
Phosphazene base P1-t-Oct
1-Ethylcyclopentanol
2-(TRIPHENYLPHOSPHORANYLIDENE)SUCCINIC ANHYDRIDE
1,8-Bis(dimethylamino)naphthalene ,99%
5-(4-Methoxyphenyl)-1H-pyrazole-3-carboxylic acid ,97%
Potassium (2-naphthalene)trifluoroborate 96%
Dimethyl Tetrachloroterephthalate
1-Amino-2-bromo-4-hydroxy-9,10-anthraquinone
4'-Methylacetophenone
CORONENE
METHYL THIOPHENE-2-CARBOXYLATE
Benzyloxyacetyl chloride
5-BroMo-5'-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-2,2'-bithiophene
3,5-Difluoro-2-hydroxyphenylboronic acid
Cyanuric chloride, 99%
3,5-DiMethyl-4-heptanone, 97%
DL-Cysteine Hydrochloride Monohydrate
BenzyltriMethylaMMoniuM Chloride
1,3-Di-tert-butylbenzene
(2,4-dibutoxyphenyl)boronic acid
Phosphoenolpyruvic acid tris(cyclohexylammonium) salt
Helicid
2-Methoxy-3-methylpyrazine
5-BroMo-3-nitro-2-propylaMinopyridine
AMMoniuM 2-hydroxyethanesulphonate
TERBIUM CHLORIDE
4-Hydroxybenzamide
2-Methyl-5-nitrobenzotrifluoride
3-(2-Chloro-3,4-dimethoxyphenyl)prop-2-enoyl chloride
1-benzyl-1H-pyrazol-3-amine(SALTDATA: FREE)
Boc-L-2-allylglycine dicyclohexylamine salt
1,3-BIS(4-NITROPHENYL)UREA
2-AMINO-5-CHLORO-3-PICOLINE
N-(Trimethylsilyl)imidazole
Cyclopropyl Methyl carbinol
1-Acetylcyclohexene
n-Octyl 2-Furancarboxylate
(1R,2R)-2-AMinocyclohexan-1-ol
Methyl benzenesulfonate, 99%
1-Naphthylamine-7-sulfonic acid
1,4-Bis(triMethylsilyl)-1,3-butadiyne
2-chloro-6-(trichloromethyl)pyridine
Fluorescent brightener 85
1,4-bis(triethoxysilyl)benzene
2-Acetylfluorene
RXR ELISA Kit
AACT ELISA Kit
Human REG-4 ELISA Kit
Human NON ELISA Kit
Chicken Laminin,LN ELISA Kit
TLR-4 ELISA Kit
SOD ELISA Kit
Human CCSA-3 ELISA Kit
Human ANG (Angiogenin) ELISA Kit
ANGPT2 ELISA Kit
Miller Medium
NBB Agar Base,Modified
(S)-Metolachlor
Glycine
5-Chloro-2-fluorobenzeneboronic acid pinacol ester, 96%
2-Fluoro-6-(TRIFluoroMETHYL)BenzylaMine
1,5-diphenyl-3-thiocarbonohydrazide
Solvent Orange 56
Poly(hexamethylene sebacamide) (Nylon 6/10)
6-fluoroquinoline
1,1,3,3-TETRAISOPROPYLDISILOXANE
4,4'-DINITROBIBENZYL
Ethyl 4-MethylcinnaMate
6-Nitro-3,4-dihydro-2H-1,4-benzothiazine, 97%
3-(2-Pyridyl)-D-alanine, 95%
4-AMino-2,3,5,6-tetrafluorobenzoic acid, 98%
Growth HorMone Releasing Factor rat
4-HEXEN-3-ONE
CalciuM forMate
N-ACETYL-D-TRYPTOPHAN
Ethanesulfonic acid sodiuM salt Monohydrate
Bupivacaine Hydrochloride
5-Methyl-isoxazole-4-carbonyl chloride
Mordant Blue 9, pure
5-Iodo-1H-pyrrolo[2,3-b]pyridine