Karpas-299细胞系*人间变性大细胞系专业性培养 货号:GD-C15718479
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞系*而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞系*加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,3-Dihydroxynaphthalene, 98%
N4-Isobutyryl-2′-deoxycytidine
3-Chlorophenoxyacetic acid
ETHYL IMIDAZOLE-2-CARBOXYLATE
Nitrosoguanidine (wetted with ca. 20% Water, containing 5g on a dry weight basis)
4,4'-Bis(4-aMinophenoxy)biphenyl
1-EthyladaMantane , 98%
1-Ethyl-3-methylimidazolium ethylsulfate, 98%
Pyrazolidine, N1-BOC protected
Trimethyl Orthobutyrate
2-Methoxy-5-(trifluoromethoxy)phenylboronic acid
2-ETHYLPHENYLBORONIC ACID
ETHYL 4,4-DIFLUOROCYCLOHEXANECARBOXYLATE
Ammonium sulfate
HOMOPHTHALONITRILE
N,N-DiMethylforMaMide dipropyl acetal
4'-DiethylaMinoacetophenone
2-BroMo-1-(Morpholinocarbonyl)benzene
RhodaMine B, pure
Kaolin, Powder, USP
2-AMino-2',4-dichlorodiphenyl Ether
4-fluorobenzophenone
Diethylaminoacetaldehyde diethyl acetal
tert-Butyllithium
3-Mercapto-1,2-propanediol
(R)-N-(tert-Butoxycarbonyl)-2-phenylglycinol
5-(2-BROMOPHENYL)-1H-TETRAZOLE
Echt Brown
3,4-Di-O-acetyl-6-O-tert-butyldiMethylsilyl-D-glucal, 97%
TriphenylMethyl Mercaptan, 98+%
(1,2-DibroMoethyl)benzene, 97%
L-Tryptophan
1,10-PHENANTHROLINIUM CHLORIDE MONOHYDRATE
trans-β-Styrenesulfonyl chloride
QuadraSil? TA
5-Carboxylthiophene-2-boronic acid pinacol ester
1-BROMO-4-TERT-BUTOXYBENZENE
N-(3-Formyl-pyridin-2-yl)-2,2-dimethyl-propionamide
(S)-1-Benzyl-3-isobutyl
2-Amino-5-nitro-1H-benzimidazole
5-iodo-1-methyl-1H-tetrazole(SALTDATA: FREE)
3-Bromo-2-methoxy-5-nitropyridine
Methandriol dipropionate
Methyl 4-iodo-2-[3-(4-methoxy-6-methyl-1,3,5-triazin-2-yl)ureidosulfonyl]benzoate sodium salt
5-[(2-Hydroxyethyl)amino]-o-cresol
Poly(diallyldiMethylaMMoniuM chloride) solution
2,5-DibroMofuran
1-(4-BroMophenylsulfonyl)piperidine
SodiuM phosphate dibasic dihydrate, 99%, for analysis
L-ValinaMide Hydrochloride
Bis(triMethylsilyl) Malonate
carnitine
Tris-(2-cyanoethyl)-phosphine
DL-Glutamic Acid
2-methylpyrazine
2-N-Boc-Amino-3-(4-tetrahydrothiopyranyl)propionic acid
4-Hydroxy-1-(2-nitro-5-trifluoroMethylphenyl)piperidine
Tetrahydrofurfuryl acrylate
AluMiniuM broMide
6-Quinolinecarbaldehyde , 97%
2-Chloro-4-(trifluoromethyl)benzenesulfonamide
(3S,5R)-7-(3-(4-fluorophenyl)-1-isopropyl-1H-indol-2-yl)-3,5-dihydroxyhept-6-enoic acidsodium salt
5-Cyanooxindole
Aluminon
Pentetrazol
(-)-CIS-2-BENZYLAMINOCYCLOHEXANEMETHANOL
Anthranilic acid
2,3-Dihydro-1-benzofuran-5-yl isocyanate
(R)-(-)-1,1'-Bi-2-naphthol Bis(trifluoroMethanesulfonate)
N-Methylbis(trifluoroacetaMide) [Trifluoroacylating Agent]
7-MethyliMidazo[1,2-a]pyridine-2-carboxylic acid
4-Chlorophenoxyacetyl chloride, 90%, tech.
THYROID
1-Octadecene
Polyphosphoric acid
3-(Methylseleno)-L-alanine
2,3-Dichlorobutane
hexylmagnesium bromide
MUC7 ELISA Kit
Mouse GAL6 (Galectin 6) ELISA Kit
Human TNP2 ELISA Kit
Human High mobility group protein B1,HMGB-1 ELISA Kit
T ELISA Kit
HSPA8 ELISA Kit
APOF ELISA Kit
Human sp185/Her2 ELISA Kit
FGβ ELISA Kit
UPA ELISA Kit
用于样品制备
Aeromonas Medium Base (Rayn)
n-HexyllithiuM, 33% (ca 2.47M) soln. in hexane
Cycloheptene, 96%, stab. with 0.1% BHT
5-Hydroxyquinoline
2-Naphthol, 98+%
4-fluorobenzophenone
a-D-Xylopyranose
6-methoxy-2,3-dihydro-4H-chromen-4-one
Itaconic Acid
Pyridine hydrobromide
TRIPHENYL(PHENYLETHYNYL)LEAD(IV)
Isopropenyl Acetate
2-AMino-6-iodopurine, 97%
Lead(II) cyclohexanebutyrate, 94%
4-Chloro-3-fluoropyridine
2-Hydroxy-5-Methoxybenzaldehyde
Glucan froM Black Yeast
1-Iodo-3,5-diMethylbenzene
N-t-Butyl 3-broMobenzaMide
4-BroMo-3-fluorophenol
2,3-Dibromo-5-methylpyridine
2-(Trifluoromethyl)benzhydrol 97%
3-(3-Methylphenyl)-1-propene
6-Bromo-quinoline-2-carbonitrile