143B TK细胞系*人胸腺激酶缺陷细胞系专业性培养

143B TK细胞系*人胸腺激酶缺陷细胞系专业性培养 货号：GD-C15719240

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系*其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Methyltin trichloride, 97%
MagnesonⅠ
4,4-DiaMino-3,3-difluorobiphenyl
3,5-DiMethyl-4-Methoxy-2-pyridineMethanol
3-AcetaMidoaniline
CYCLOOCTANONE OXIME
1-Chloro-2-Methoxy-4-Methylbenzene
2-Isopropyl-6-methylphenyl isothiocyanate
(S)-(-)-[4,5-Dihydro-4-(1-methylethyl)-2-oxazolyl]ferrocene, min. 98%
Lithium hydroxide ,99% [anhydrous]
3-Bromoheptane
Cefazolin
Pyrazole
N-BUTYLCYCLOHEXANE
1,4-DibroMobutane
Methyl 4-Acetylbenzoate
2,5-DiMethyl-3-furancarboxylic Acid
Methyl 4-broMo-3-fluorobenzoate
Zinc broMide, 99.999%, (trace Metal basis), extra pure
Stannous Pyrophosphate
9-Heptadecanone
3-Nitrophthalic anhydride
2-Thiazolidinethione,3-(1-methylethyl)-
Glyoxalbis(2-hydroxyanil)
1,1'-methylenebis[4-fluorobenzene]
2-(N-PROPYLTHIO)ETHANOL
1-BroMo-2-Chloroethane

4'-Fluorovalerophenone, 97%
IridiuM(IV) iodide, PreMion|r, 99.95% (Metals basis), Ir 27.0% Min

4-N-PENTYLOXYBENZALDEHYDE
3-TrifluoroMethylthiobenzaMide
O-Phospho-L-serine
DUROHYDROQUINONE
1,3,5-Trifluorobenzene
4-Aminomethyl-2-fluorobenzonitrile
4-Bromo-2-fluorophenylhydrazine hydrochloride
4-Amino-3-fluorobenzenethiol
Sodium permanganate monohydrate, 97%
3-Aminopropylphosphonic acid
N-BROMOACETAMIDE
1,2-BIS(1-NAPHTHYL)ETHANE
2,5-Dimethoxyaniline
3,4-Dihydro-2H-1,4-benzoxazin-2-ylMethanol
CyclopentylMalonic Acid
3-(N-MethylaMinocarbonyl)phenylboronic acid, pinacol ester
EthylaluMiniuM sesquichloride, 0.4M solution in hexane, AcroSeal
1-Benzo[b]thiophen-3-yl-2-broMoethan-1-one, 97%
3,5-Dichloro-4-hydroxybenzoic Acid
4,4'-Bis(4-aMino-1-naphthylazo)-2,2'-stilbenesulfonic Acid [Indicator for Organic Acid Anhydrides by Morpholine Method]
n-Octyl Methacrylate
Dicyclopentadiene
hafnium trifluoromethanesulfonate
Fmoc-D-Pipecolic acid
2,3-Pyrazinedicarboxylic acid
4-VINYLBENZENESULFINIC ACID SODIUM SALT
1,3-Bis(MethoxyMethyl)-2,4-dioxo-1,2,3,4-tetrahydropyriMidine-5-boronic acid
1-(4-CHLOROPHENYL)-1-CYCLOPROPANECARBONITRILE
(S)-(+)-(3,5-Dioxa-4-phospha-cyclohepta[2,1-a;3,4-a']dinaphthalen-4-yl)dimethylamine, min. 97% (S)-MONOPHOS
Potassium hexanitritorhodate(III), min. 97%
Diethyl pyridine-3,5-dicarboxylate
3-Amino-4-chlorobenzenesulfonyl fluoride, 98%
3-(1-(Dimethylamino)ethyl]phenol
NICOTINOYL-GLYCINE
4-(TRIFLUOROMETHYL)THIOPHENOL
Vinylmagnesium bromide
IodoMethylcyclopentane
1-BroMo-3-Methoxypropane
3-(4-Ethoxycarbonylbutyloxy)-2,4,6-trifluorophenylboronic acid
Deoxybenzoin, 98%
4-tert-Butylbenzaldehyde, 95%
4,7-Dichloroisatin
L-(+)-Lysine Monohydrochloride
2-Chloro-N-(2,6-dimethylphenyl)acetamide
7-Azidoheptanoic acid ethyl ester
DPQ
N-[1-(S)-Ethoxycarbonyl-3-Phenylpropyl]-N-Carboxy-L-Alanine Anhydride (Nepa-Nca)
TACR2 ELISA Kit
S100A13 ELISA Kit
Human PCDHβ8 ELISA Kit
Human ReverseTri-iodothyronine,rT3 ELISA Kit
Chicken α-Glucosidase,a-Glu ELISA Kit
β2-GP1 Ab lgG ELISA Kit
Chicken HP (Haptoglobin) ELISA Kit
Human CCKBR (Cholecystokinin B Receptor) ELISA Kit
Human PTX 3/TSG-14 (Pentraxin 3, Long) ELISA Kit
PL ELISA Kit
Salt Tolerance Test Medium Base
Thioglycollate Broth Medium
Citroflex A-4
Caesium fluoride
5,6,7,8-Tetrahydrobenzo[b]thieno[2,3-d]pyriMidine-4(3H)-thione, 96%
2-Fluoro-4-iodoaniline, 99%
3-Nitrophthalic anhydride
N-alpha-t-BOC-L-glutamic-gamma-benzyl ester
Saponin
Cycloheptanone

5-Amino-1,3,4-thiadiazole-2-thiol
TRIMETHYLENE OXIDE
4,4-Bipyridine
Xenon difluoride, 99.5% (Metals basis)
4-Fluoro-3-(hydroxyMethyl)benzeneboronic acid, 98%
Pentostatin
Boc-Pro-OH
Copper(II) 2,3,9,10,16,17,23,24-Octafluorophthalocyanine (purified by subliMation)
N-CARBOBENZOXY-L-SERINE BETA-LACTONE
3-PyridineMethanol
5-(4-Chlorophenyl)oxazole-4-carboxylic acid
6-Nitro-1,3-dihydroisobenzofuran-1-one, 97%
1-isopropyl-1H-pyrazole-4-carbaldehyde(SALTDATA: FREE)
(R)-N,N-Dimethyl-1-[(S)-2-(diphenylphosphino)ferrocenyl]ethylamine
1-(4-Chlorophenyl)-5-(trifluoromethyl)-1H-pyrazole-4-carbonyl chloride 97%