Hs68细胞系*人男性正常龟头细胞系专业性培养 货号:GD-C15719175
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系*其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Methoxybenzenesulfonyl chloride, 98+%
(S)-Ru(OAc)2(T-BINAP)
2-AcetaMido-2-deoxy-D-glucose
6-AMino-2-Mercaptobenzothiazole , 95%
Tetrahydro-2-Methyl-3-thioxo-1,2,4-triazine-5,6-dione
N-DODECYL CYANIDE
BIS(PENTAMETHYLCYCLOPENTADIENYL)IRON
4-Fluoro-2-(trifluoromethyl)cinnamic acid
2-(Trifluoromethyl)benzoic acid hydrazide 95%
1,4-Bis(bromomethyl)naphthalene (contains ca. 23% isomer)
8-Bromo-3,4-dihydro-2H-naphthalen-1-one
5-Amino-4-hydroxybenzene-1,3-disulphonic acid
Butyl acetate
Tiagabine
1,3,5-Benzenetricarboxylic acid
2-Ethoxyethyl ether
1,3-Di-tert-butyliMidazoliuM Tetrafluoroborate
M-Xylylene Diisocyanate
Tyloxapol
SodiuM Erythorbate, FCC
(R)-1,1'-Binaphthyl-2,2'-disulfonyl Dichloride
1,3-diethylurea
lithium citrate hydrate
3-Amino-2,4-dimethylpyridine
2,4-Difluorotoluene
1-IODO-4-NITROBENZENE
3-(3,4-DIHYDROXYPHENYL-2,5,6-D3)-L-ALANINE
DisodiuM Hydrogenorthophosphate
3-Iodophenylacetonitrile, 97%
4,5-Difluoro-2-Methoxybenzoic acid, 97%
(R)-N-FMOC-4-BroMophenylalanine
Ethyl iMidazole-2-carboxylate
P-CHLOROPROPYLBENZENE
PERFLUORO-N-HEPTYL IODIDE
3-(4-Nitrophenyl)-5-(thiophen-2-yl)-1,2,4-oxadiazole
3,4-DIMETHYL-O-PHENYLENEDIAMINE
Bis(cyclopentadienyl)dimethylzirconium(IV)
1-(Bromomethyl)adamantane, 97%
Di-1-adamantylphosphinic chloride, 98%
3-(2-Amino-ethyl)-aniline
4-CHLORO-2-METHYLPHENYLBORONIC ACID
SUCCINALDEHYDE
METHYL MERCAPTAN
Wood alcohol
Dibutyltin Dichloride
4-Cyanobenzaldehyde
N-ForMyl-L-valine
4-Chlorothiophene-2-carboxylic acid
DichlorodiMethylgerMane, 97%
Silver AtoMic Absorption Standard
N-Carbobenzoxy-DL-valine
L-serine benzyl ester hydrochloride
Tetrabutylammonium bromide
H-Glu(OtBu)-OH
6-Chloro-1H-indazole
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride
Fluorobenzene
N-ACETYL-2-PHENYLETHYLAMINE
2-Nitrobenzenesulfonyl chloride
3-(Phenylthio)acrylic acid, Mixture of cis and trans
1-(3-Methoxybenzyl)-2-thiourea
Ammonium persulfate, extra pure, 98%
2-Hexynoic Acid
3,5-Dichloro-4-hydroxypyridine
(R)-(-)-6,6'-Dibromo-1,1'-bi-2-naphthol
1-PHENYL-1-BUTYNE
2-Bromonaphthalene
2-Nitro-p-phenylenediaMine
1-Benzyl-2-pyrrolidinone
9,10-Dihydro-9,10-bis(2-carboxyethyl)-N-(4-nitrophenyl)-10,9-(epoxyiMino)anthracene-12-carboxaMide
4-BroMo-3-ethoxybenzaldehyde
3,4-Dihydro-2H-1,4-benzoxazine-2-carbonitrile, 97%
SulfaMethoxazole, Micronized, USP
MonosodiuM Maleate Trihydrate
5-Nitro-2-furoic acid
1-aminofluorene
testosterone isobutyrate
3,5-Pyridinedicarboxylic acid
GPBB ELISA Kit
Thy1 ELISA Kit
Human apo-A2 ELISA Kit
Human ARSI ELISA Kit
Chicken IL-23 (Interleukin 23) ELISA Kit
AHSG ELISA Kit
Chicken BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
Human MCP3 ELISA Kit
Human angiostatin,ANG ELISA Kit
PF3 ELISA Kit
Brain Heart Infusion Broth
0.5% Glucose Broth Medium
DL-Isoborneol
AMMoniuM hexafluorotitanate
5-BENZYLOXY-1-PENTANOL
2-Fluoro-5-iodobenzoic acid, 97%
1,3-diethylurea
4-(methylthio)benzoic acid
Zinc Nitrate Hexahydrate
4-nitrobenzenediazonium tetrafluoroborate
Celliton Fast Pink B
3-(3-Indolyl)-2-oxopropanoic acid
4-Phenoxyphenol
5,5-DiMethyl-1,3-dioxane-2-butanal, 96%
4-BroMo-2-fluorobiphenyl, 98%
AMylose froM potato
4,4′-Thiobisbenzenethiol
N-HEXADECENYLSUCCINIC ANHYDRIDE
(S,S)-N-(p-Toluenesulfonyl)-1,2-diphenylethanediaMine(chloro)(p-cyMene)rutheniuM(II)
BIS(4-NITROPHENYL) SULFIDE
2,4-Dichlorobenzyl isocyanate
Oxalic acid dihydrate, ACS grade
(1-Methyl-1H-pyrrol-2-yl)methylamine, 97%
Calcium carbide, 97+%
Phenyl Vinyl Sulfide (stabilized with TBC)