EAG3细胞系*人子宫内膜低分化腺癌细胞系行业更低价 货号:GD-C15618934
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系*为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-[2-(4-Morpholinyl)ethylcarbaMoyl]benzeneboronic acid hydrochloride, 98%
PotassiuM
Ferritin froM Horse Spleen (100Mg/1Ml)
2,6-Bis[(3aS,8aR)-3a,8a-dihydro-8H-indeno[1,2-d]oxazolin-2-yl]pyridine
10-UNDECENOIC ACID VINYL ESTER
3-Methoxycarbonyl-5-isobutoxyphenylboronic acid, pinacol ester
2,5-DiMethylbenzoic acid
3-Acetyl-7-hydroxy-2H-chromen-2-one ,98%
2-Oxo-4-phenylpyrrolidine-3-carboxylic acid
3-(2-Ethylhexyloxy)propylamine
Potassium hexacyanoplatinate(IV), 99%
2,4,6-Trimethylphenylacetonitrile
Ethyl 1,3-dithiane-2-carboxylate
Ginsenoside Rg3
tert-Butylhydrazine hydrochloride
4-Biphenylacetonitrile
1,2'-DinaphthylaMine
3,5-DibroMo-2-Methoxybenzoic acid
Tolazoline hydrochloride, 99%
Perphenazine, USP
5-Hydroxyflavone
3-formyl-4-hydroxybenzoic acid
Ethenylethoxydimethyl silane
(4E)-4-benzylidene-2-phenyl-1,3-oxazol-5(4H)-one
3-hydroxybutyric acid, sodium salt
4-CHLORO-8-(TRIFLUOROMETHYL)QUINOLINE
4-ETHYLTOLUENE
5-BroMo-2-cyanobenzeneboronic acid, 97%
cis-1,8-p-Menthanediol Monohydrate, 98%
3-Chloro-4-fluoro-N-(2-fluorobenzyl)aniline, 97%
o-Acetoacetanisidide
5-BroMo-N-(4-Methoxybenzyl)-3-nitropyridin-2-aMine
MECLOFENAMATE SODIUM
Riboflavin-5-phosphate sodiuM
BIS(TRIFLUORO-2,4-PENTANEDIONATO)COBALT(II)
N-Ethyl-3-carbazolecarboxaldehyde
Erbium(III) fluoride 99.9%
2-Amino-4-methyl-5-nitropyridine
H-Leu-OBzl.TosOH
Cerium(IV) sulfate hydrate, 99%
(HEPTAFLUOROBUTYRO)PHENONE
Nandrolone
3-(Trifluoromethoxy)phenol
2-Chloroethylamine hydrochloride
5-BroMo-2-furoic acid
2,2-Bis(4-oxocyclohexyl)propane
5-Carboxylthiophene-2-boronic acid pinacol ester
Anthracene, 99%
3-Cyano-5-fluorophenylboronic acid, 97%
2,2,3,3-Tetrafluoropropyl Methacrylate [stabilized with 2,2'-Methylenebis(6-tert-butyl-4-Methylphenol)]
N,N'-DibenzylethylenediaMine Diacetate
2,5-dimethylpyrrole
(2,6-dichlorophenyl)methylzinc chloride
4-oxoazetidine-2-carboxylic acid
2-Bromo-6-ethoxynaphthalene
1-(3-BROMOPROPOXY)-2-BROMOBENZENE
TantaluM wire, 2.0MM (0.08in) dia, annealed, 99.95% (Metals basis excluding Nb)
TriMethoxyboroxine
2-Chloro-4-nitrobenzoyl chloride, 98%
3-CHLORO-4,5-DIAMINOBENZOTRIFLUORIDE
1-(Bis-dimethylamino-methylene)-5-chloro-3-oxy-1H-benzotriazol-1-ium hexafluorophosphate
4-(4-Chlorophenyl)-1,2,3,6-tetrahydropyridine hydrochloride, 97%
2,6-Di-tert-butyl-4-hydroxymethylphenol
2,4,6-Trifluorobenzyl bromide 97%
1,1,1-TRIFLUORO-2-BUTANONE
2-Chloro-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide
DYSPROSIUM
2,4-Difluorobenzaldehyde
1,3-Cyclohexanedione
Dansyl-L-phenylalanine
6-BroMo-2-Mercaptobenzothiazole
PiperonylaMine, 97%
Evans Blue
Methyl Anthranilate
acridin-9-ylamine hydrochloride
1-Chloro-3,3-dimethylbutane
Arbutin
Iodoacetic acid
FABP3/FABP11/MDGI ELISA Kit
Mouse Beta-Endorphin receptor,β-EPR ELISA Kit
LAM ELISA Kit
Human BA ELISA Kit
Chicken Immunoglobulin A,IgA ELISA Kit
PKM2 ELISA Kit
Chicken 17-ketosteroids,17-KS ELISA Kit
Human estrogen,E ELISA Kit
Human vasopeptidase inhibitors,VPI ELISA Kit
SLE ELISA Kit
Malt Extract Agar Base
RPF Agar Medium Base
Isonicotinic acid
CeriuM zirconiuM oxide, 99.5% (Metals basis)
5-AMino-2-Fluorobenzioc Acid
2-HEXANOL
3-formyl-4-hydroxybenzoic acid
Decanoic acid, cobalt salt
Ethyl 3-amino-3-oxopropanoate
D-(-)-Arginine
2,6-Dichloroindophenol sodium salt
4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)CHLOROBENZENE
4-Hydroxy-3-Methoxy-5-Nitrobenzaldehyde
PraseodyMiuM(III) perchlorate, 50% w/w aq. soln., Reagent Grade
Farnesol, Mixture of isoMers, 96%
Ethyl acetohydroxaMate
Cyclohexanone
4-PHENOXYPIPERIDINE
ETHANOL-D6
1,1-DIPHENYL-2-PICRYLHYDRAZYL
TEREPHTHALIC ACID MONOMETHYL ESTER POTASSIUM SALT
4-Fluoro-3-(trifluoromethyl)phenol
4-(Trifluoromethyl)-1,4,5,6-tetrahydropyridine-3-carboxamide, 97%
Dichlorobis(2-(diisopropylphosphino)ethylamine)ruthenium(II), 97%
3-Isochromanone