PL-1细胞系*人骨肉瘤细胞系促销价供应 货号:GD-C15519719
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞系*说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞系*就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞系*肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞系*刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
PraseodyMiuM oxide
2-Hydroxy-5-nitropyridine
N,N,N',N'-TETRAMETHYL-2-BUTENE-1,4-DIAMINE
1-Pyrenylboronic acid
(S)-(+)-1,1'-Binaphthyl-2,2'-diyl hydrogenphosphate
3-(DIMETHYLAMINO)BENZOIC ACID METHYL ESTER
2-Bromo-3-methoxynaphthalene
(1R)-1-[4-(Trifluoromethyl)phenyl]ethan-1-ol
O-Methylisourea hemisulfate, 94%
Ethyl (S)-Nipecotate D-Tartrate
2-[[3-Chloro-5-(trifluoromethyl)-2-pyridinyl]-(methyl)amino]-1-ethanol
PYRIDINIUM FLUOROCHROMATE
Aloe-emodin
1'-Acetonaphthone
2,4,5-Trifluoronitrobenzene
2-Mercaptobenzoxazole
5'-Deoxy-5-fluorocytidine
5-AMino-4-cyano-3-Methyl-1-phenyl-1H-pyrazole
Methyl pentadecafluorooctanoate, 95%
1,2-DIMETHYLNAPHTHALENE
TriMethylsilyl Acetate
1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine
3-Methoxy-4-methylbenzoic acid
2-bromo-3,3,3-trifluoropropene
1,6-Dimethylnaphthalene
ISOQUINOLINE-3-CARBOXYLIC ACID HYDRATE
1-Methyl-4-PiperidineMethanol
6-FluorochroMone, 97%
7-Methoxy-4-MethylcouMarin, 99%
CadMiuM ingot/button, ^=36MM (1.4in) dia x 8MM (0.3in) thick, 99.9% (Metals basis)
Methyl isoquinoline-3-carboxylate
7-Acetoxy-6-(2,3-dibroMopropyl)-4,8-diMethylcouMarin
trans-CinnaMic acid
NESSLER'S REAGENT
2,6-Difluorobenzenesulfonyl chloride
Bis(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate
2-(2H-tetrazol-5-yl)aniline(SALTDATA: FREE)
2-Chlorobenzylamine 97%
Monopalmitin
N,N'-Diethylethylenediamine
Cialis
3-Chloro-4-morpholino-1,2,5-thiadiazole
Bis[3-(triethoxysilyl)propyl]amine
TRICARBALLYLIC ACID
Neopentyl broMide
2,6-Bis(hydroxyMethyl)-1,4-diMethoxybenzene
2-Chlorothiophene-3-boronic acid
Catechol, 99+%
ODQ, 98%
MethoxycarbonylMethyl(triphenyl)phosphoniuM BroMide
3-Hydroxybenzoic Acid
5-methyl-2'-deoxycytidine
(3-fluoro-5-methoxyphenyl)boronic acid
7-Ethyl-10-hydroxycamptothecin
3-bromo-7-nitro-2H-indazole
Diphenyldimethoxysilane
Benzyl cinnaMate
JP-TS AVIATION FUEL
4-Cyano-2-fluorophenylboronic acid, pinacol ester
2-Bromo-6-ethoxypyridine
2,6-Dichlorobenzoyl Chloride
2,3-Dihydro-6-isocyano-1,4-benzodioxine, 95%
2,5-Dichloroterephthalonitrile
METOLCARB
Algestone acetophenide
DODECYLBENZENE
INDOLE-3-GLYOXYLYL CHLORIDE
2,6-Di-tert-butyl-4-Methylphenol
1-Penten-3-yl Acetate
1,3,4,6-Tetra-o-acetyl-2-aMino-2-deoxy-beta-d-glucopyranose, HCl
4,7-Diphenyl-1,10-phenanthroline, 99%
PotassiuM chlorochroMate, 98%
1,2-BENZANTHRACENE
Methyl alpha-BroMophenylacetate
Methyl Formamide
entecavir
Sodium bromocresol green
2-(3-chloropropoxy)tetrahydro-2H-pyran
HSPA1A ELISA Kit
Mouse FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit
Human hnRNP G ELISA Kit
Human D3R/DRD3 (Dopamine Receptor D3) ELISA Kit
Monkey MYO (Myoglobin) ELISA Kit
IL13 ELISA Kit
Ntn1 ELISA Kit
Human Proteinase 3 Antibody,PR3Ab ELISA Kit
PDGF-BB ELISA Kit
Beclin 1 ELISA Kit
Skirrow Agar Base
Skirrow Agar Base
Magnesium acetate tetrahydrate
Phenyl trans-^b-styryl sulfone, 98%
4-n-Butylphenol, 98%
2-Phenoxyethyl chloride, 97%
1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine
2-sec-butylcyclohexanone
DL-1-Phenylethanol
2,4'-Dipyridyl
2-Thiouracil
2-METHOXYPHENYL CHLOROFORMATE
2-BroMo-5-Fluorobenzyl BroMide
Propionic anhydride, 97%
4-Fluoro-3-iodobenzoic acid, 97%
DiMethyl sulfate-d6
2,4-Dichlorobenzoyl chloride
4-CYANO-4-(4-FLUOROPHENYL)CYCLOHEXANONE
3-Propoxyphenylboronic acid
4-AMino-2-nitrophenol
2,3-DiaMino-5-broMopyrazine
2-Acetyl-5-methylthiophene
2-Bromobenzonitrile 99%
Dansyl-L-threonine Piperidinium Salt
Cyanoacetohydrazide