HT-1080[HT1080]细胞系*人成纤维肉瘤细胞系开学季减价 货号:GD-C15220398
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,6-Dichloro-4-nitroaniline, 95%
BetaMethasone 17-valerate
Methyl Malonyl chloride
Vinylcyclooctane
Solvent Green 5
HYDRIDOCARBONYLTRIS(TRIPHENYLPHOSPHINE)IRIDIUM (I)
AllyltriphenylphosphoniuM broMide
(1R,2R)-2-(Diphenylphosphino)-2,3-dihydro-1H-inden-1-amine, min. 97% (10wt% in THF)
(3'-Methoxy-biphenyl-4-yl)-acetic acid
1-Amino-1-phenylpropan-2-one hydrochloride
4-(n-Pentyloxy)benzeneboronic acid
(S)-6,7-Dimethoxy-1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid hydrochloride
1-(2,4-DICHLOROBENZYL)
MECARBAM
2-Hydroxy-4-MethylpyriMidine hydrochloride
2-Chloro-4-fluorobenzoic acid
1,3-DicyclohexyliMidazoliuM Tetrafluoroborate
1-Boc-2-piperidinecarbaldehyde
YttriuM(III) nitrate hexahydrate, 99.9%, (trace Metal basis)
Squalane, NF
2-Naphthaleneboronic Acid (contains varying aMounts of Anhydride)
reichstein'S substance S 21-acetate
4-Methylbenzamidine hydrochloride
columbianetin
3-Fluorobenzaldehyde
(S)-(-)-2-METHOXYPROPIONIC ACID
1H-Purin-6-amine sulfate
SodiuM phosphotungstate octadecahydrate
2,4-DibroMo-N-(4-fluorobenzyl)aniline, 97%
N-Acetyl-L-tyrosine ethyl ester Monohydrate, 99%
1,5-Pentanedithiol, 96%
Isopropenyl chloroforMate
HEXAFLUORO-2-(P-TOLYL)ISOPROPANOL
O-TOLUALDEHYDE 2,4-DINITROPHENYLHYDRAZONE
TRIARACHIDIN
Dipropylene glycol MonoMethyl ether
3-(2-FURYL)-2-PHENYLPROPENAL
5-Bromosalicylaldehyde ,99%
3,6-Dichloro-benzo[b]thiophene-2-carbonylchloride
2-Bromo-4-fluoro-5-nitrophenol
1-adamantyl hydrosulfide(SALTDATA: FREE)
tert-Butyl L-alaninate hydrochloride
4-(Trifluoromethoxy)benzoic acid
2,4,6-TRIMETHYL DIPHENYL SULFIDE
TERT-BUTYL METHYL MALONATE
Phenylurea
Dansyl Fluoride
5-BroMo-2-phenoxypyridine
SeleniuM, 99.5+%, powder, 200 Mesh
LeMon Oil, Italian Type, Coldpressed, FCC
1,3,5-Triethylbenzene
p-Fluorotoluene
benzyl 2,4-dihydroxyphenyl ketone
3-Methyl-2-butylamine
Tetraethylthiuram disulfide
Trimethylsilyl trifluoromethanesulfonate
4-(Azetidin-1-ylsulfonyl)phenylboronic acid
Indigo
5-Nitro-8-(pyrrolidin-1-yl)quinoline
1,3-Dioxan-2-one
5-tert-Butoxycarbonylamino-pyridine-3-carboxylic acid ,97%
N-Methylhexylamine
H-Orn(Z)-OH
1H,1H-PERFLUORO-1-HEPTANOL
Edifenphos
4-Vinyl-1-cyclohexene
3-BUTOXYBENZENEBORONIC ACID
3-Cyclopentylpropionic acid
1-BroMo-3-chloro-2-Methylpropane
5-Chloro-2-cyanophenylboronic acid
Benzothiazole, 97%
2-Octyn-1-ol, 98%
N-(2,4-Dinitrophenyl)-L-leucine
2,5-DiMethyl-3-hexyne-2,5-diol
Tetrafluorophthalic acid
5-Chloro-2-thiophenecarboxylic acid hydrazide
Milnacipran Hydrochloride
1-(4-Cyanophenyl)-
AFGF-1 ELISA Kit
S100A7 ELISA Kit
Human TPM3 ELISA Kit
Human UBN1 ELISA Kit
Chicken Interferon β,IFN-β/IFNB ELISA Kit
β2-GP ELISA Kit
CT ELISA Kit
Human cholecystokinin octapeptide,CCK-8 ELISA Kit
Human PC ELISA Kit
PTF1α ELISA Kit
BTB Medium
Casein peptone agar (G.A)
Vanillin acetate
CeriuM(III) fluoride, anhydrous, 99.9% (REO)
5,6,7-TriMethoxyflavone, 97%
2-Fluoro-4-iodobenzonitrile, 98%
reichstein'S substance S 21-acetate
Alpha-Cedrene Epoxide
4-Cyclopropylphenol
4-Hydroxyphenethyl alcohol
BIS(CYCLOPENTADIENYL)TUNGSTEN DIHYDRIDE
NICKEL(II) AMIDOSULFATE TETRAHYDRATE
2-AMino-3-benzyloxypyridine
(+)-1,2-Bis[(2S,5S)-2,5-diethylphospholano]benzene(1,5-cyclooctadiene)rhodiuM(I) tetrafluoroborate, 97%
3-Fluorophthalic anhydride, 98%
4-Methoxyacetophenone
TetraethylthiuraM disulfide
2-METHOXYFURAN
2,6-DIFLUORO-3-METHYLPHENYLACETIC ACID
4-(N-BUTOXYCARBONYL)PHENYL ISOCYANATE
3-Cyanophenylboronic acid, pinacol ester
(1S)-(-)-1-(Naphth-1-yl)ethylamine
6-Bromo-1H-indole-2,3-dione
2-(Chloromethyl)-5-methylthieno[2,3-d]pyrimidin-4(3H)-one ,97%
Poly(4-chlorostyrene)