Pt-K2(PtK2)细胞系*袋鼠肾细胞系开学季大放送 货号:GD-C15120553
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-(2,3-Difluorophenyl)ethanol, 97%
2-Cyanobenzeneboronic acid
DiethylenetriaMinepentakis(Methylphosphonic acid) solution
Methyl 4-chloro-2-fluorobenzoate
(S)-tert-ButanesulfinaMide
3-(2-Methoxybenzyloxy)phenylboronic acid
p-Xylene [Tag Closed Flash Point Check Fluid]
Barium iodide, anhydrous, min. 97%
3-Pyridin-4-yl-propionic acid
2-Chloro-5-(trifluoromethyl)anisole
2-Chloro-1-piperidin-1-yl-ethanone
(3,4-Diaminophenyl)phenylmethanone
N-Acetylneuraminic acid
6-Methoxypurine
3,5-DiMethyl-4-iodo-1H-pyrazole
2,3-Dicyanohydroquinone
3-(DiMethylcarbaMoyl)phenylboronic Acid (contains varying aMounts of Anhydride)
tert-Butyl 2-broMo-4-fluorobenzoate
Tetrahydrofurfuryl chloride, 98+%
Tylosin Tartrate, For Veterinary Use, BP
4-Methyl-2-hexene (cis- and trans- Mixture)
bismuth(iii) citrate
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
Bismuth subnitrate
3-butylpyridine
3-TERT-BUTYL-1-METHYL-2-PYRAZOLIN-5-ONE
Titanium tetraisopropanolate
7-Fluorooxindole
SodiuM n-heptafluorobutyrate, 98%
Bis(tert-butylcyclopentadienyl)diMethylhafniuM(IV), 98+%
2-AMinopyridine
4-Methylpyridine-2-carboxaldehyde
2'',4'',6''-TRIFLUOROPROPIOPHENONE
N-Methoxy-N-MethylbenzaMide
3-AMino-L-tyrosine dihydrochloride Monohydrate
(S)-3,3-Diphenyl-1-Methylpyrrolidino[1,2-c]-1,3,2-oxazaborole
3-(Methoxycarbonyl)-5-nitrobenzeneboronic acid
4-(4-Propylphenyl)benzoic Acid
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl, 99+%
Potassium niobate (99.999%-Nb) PURATREM
2,4-DICHLORO-1,3,5-TRIAZINE
1-Ethyl-1H-1,2,3,4-tetrazole-5-thiol
Carbonylchlorohydrotris(triphenylphosphine)ruthenium
Carbidopa Related CoMpound A;3-O-Methylcarbidopa
cis-2-(tert-ButoxycarbonylaMino)-1-cyclopentanecarboxylic acid
3-Acetylthiazolidine-2-thione
5-(AMinoMethyl)-2-fluorophenylboronic acid, HCl
4-Fluorobenzyl broMide, 97%
2-AMino-5-Methoxybenzoic acid, 97%
1,3,6,8-PYRENETETRASULFONIC ACID TETRASODIUM SALT
3-Fluorobenzoic Acid
1H,1H-pentadecafluorooctyl acrylate
2'-FLUORO-5'-O-DIMETHOXYTRITYL-N4-ACETYL-D-CYTIDINE
4-Bromo-3-chloropyridine
2-Imidazolidinone, 1-(4-piperidinyl)-
2,3-DIFLUORO-6-NITROPHENOL
2,5-Dichlorobenzaldehyde
dioctyl succinate
3-ETHYL-3-OXETANEMETHANOL
3-Phenylphenol
N,N,N',N'-Tetramethylethylenediamine, 99% TMEDA
4-(Piperidin-4-ylamino)-benzoic acid ethyl ester
16,17-Epoxyprogesterone
Adenine
2-Amino-5-nitrothiazole
D-alpha-Cyclohexylglycine
2-IMINOTHIOLANE
Pyridine-3-sulfonyl chloride hydrochloride
1H-Pyrazole-3-MethanaMine
Tricyclo[5.2.1.0(2,6)]decan-8-one
2,5-DibroMohexane, 97%, Mixture of diastereoMers
Thiocarbanilide, 98%
D-alpha-Tocopheryl acetate
4-(BroMoMethyl)-3-fluorobenzonitrile
4-Amino-3-chloro-5-trifluoromethylbenzaldehyde
D-(+)-(2-Chlorophenyl) Glycine
4-Chloro-3-nitrobenzoyl chloride
(1S,2S)-(+)-1,2-Diaminocyclohexane
NTRK2 ELISA Kit
Mouse CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
Human CRY1 ELISA Kit
Human GALNT3 ELISA Kit
Monkey SHBG (Sex Hormone Binding Globulin) ELISA Kit
Orexin ELISA Kit
VEGF-A ELISA Kit
Human PKD1 (Protein Kinase D1) ELISA Kit
Human GP-ⅡbⅢa/CD41+CD61 ELISA Kit
TNFsR-I ELISA Kit
LB Broth,Miller
Brilliant Green Lactose Bile Broth
n-Propyl acetate, 99%
Molecular Sieve
4-n-Pentylbenzoyl chloride, 98%
2-Iodo-3-Methylpyridine, 97%
bismuth(iii) citrate
Salen-Cu
Cefoselis
2,6-Dinitrophenol
2,5-Dimethyl-2,4-hexadiene
Bis(3-nitrophenyl) disulfide
4-Methylisoxazole
Methyl 6-Methylpyridine-2-carboxylate, 96%
Bathocuproin, 98%
1,3-Dichloropropene (cis+trans)
2,3-Difluorophenetole
RhodiuM(II) acetate diMer
TRIPHENYLPHOSPHINE RESIN
TetraMethylaMMoniuM fluoride
1,5-BIS(4-NITROPHENYL)CARBOHYDRAZIDE
Samarium foil (99.9% REO)
3-Quinolinecarboxaldehyde, 97+%
1-(5-methoxy-1H-indol-2-yl)methanamine(SALTDATA: CH3SO3H)
5-Methyl-1,1,1-trifluoro-2,4-hexanedione