Hca-F细胞系*小鼠肝癌细胞系开学季大放送 货号:GD-C15120519
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-5-iodo-4-Methylpyridine, 95%
Diclazuril
Di-n-propyl succinate, 98%
(S)-(-)-4-ISOPROPYL-5,5-DIPHENYL-2-OXAZOLIDINONE
1-(2-Chloroethoxy)propane
2-Fluoro-N-Methylaniline
2,2,3,3-Tetrafluoropropyl methacrylate 97%
5-Methoxyisatin
Boc-(±)-trans-2-aminocyclohexane-1-carboxylic acid
Phenylthiohydantoin-tyrosine
2,4-Diamino-6-(2-fluorophenyl)-1,3,5-triazine
N-HEPTYLBENZENE
3-HYDROXYPROPIONIC ACID
BOC-D-2-Fluorophe
TetraoctylaMMoniuM broMide
4-BroMoaniline
(S)-O-DesMethylraclopride
2-BroMo-5-(trifluoroMethyl)benzaldehyde
BOC-2-piperidylMethanol, 97%
Tragacanth, Powder, NF
ForMoxiMe (10% in Water, ca. 2.4Mol/L)
3,4-DICHLOROPROPIONANILIDE
12-Oxa-16-hexadecanolid
2-(1H)-Quinolinone
Iodosylbenzene
4-Amino-3-hydroxybenzoic acid
CIS-2-HEXENE
6-INDAZOLYBORONIC ACID
2-AMino-6-ethylpyridine, 97%
2-Chloro-N-ethylbenzaMide, 97%
ChroMiuM chloride
Picrolonic acid
ISOPROPYL METHACRYLATE
N-MethylforManilide
N-CYANO-N',S-DIMETHYLISOTHIOUREA
3-(3-(Ethoxycarbonyl)piperidine-1-carbonyl)phenylboronic acid
Thiophene-3-carboxylic acid 98%
Tri-p-tolylsulfonium Hexafluorophosphate
3-Phenoxybenzyl chloride 98%
4-Phenylpyridine-2-carboxylic acid 97%
3-TRIFLUOROMETHYL BENZOTRICHLORIDE
VALERIC ANHYDRIDE
1-Hexyl-3-methylimidazolium chloride
Propyl butyrate
1,2-Benzisothiazol-3(2H)-one
DiMethyl 5-AMinoisophthalate
5,6-Difluoroindole-2-carboxylic acid
1-Benzylpiperidin-4-aMine, 97%
2-(4-Chlorophenyl)-2-Morpholinoacetonitrile, 97%
Carboplatin
Norbornane-2-Methanol
Levulinic acid
2-ACETAMIDO-4-METHYL-5-BROMOPYRIDINE
Brewers yeast
cis-3-hexenyl phenylacetate
O-Methylisourea hydrochloride
4-(DicyanoMethylene)-2-tert-butyl-6-(1,1,7,7-tetraMethyljulolidin-4-yl-vinyl)-4H-pyran
CouMarin
(R)-(-)-DBD-PY-NCS
2-Bromo-5-chlorobenzyl bromide
2-(Methylamino)nicotinic acid
Puerarin ,98%
N-[4-(2-Dimethylaminoethoxy)benzyl]-3,4-dimethoxybenzamide hydrochloride
6-METHYL-4-(TRIFLUOROMETHYL)-2(1H)-PYRIDONE
Calcium 2-oxoglutarate
5-BROMO-2-IODOTOLUENE
3-Chloropropionic acid;3-Chloropropanoic acid
1-(4-AMino-2-hydroxyphenyl)ethan-1-one
1-Ethyl-3-MethyliMidazoliuM DicyanaMide
Octadecanophenone
Methyl 2-aMino-5-nitrobenzoate
4-Piperidinobenzaldehyde, 97%
(+/-)-HYDROBENZOIN
Benzoylurea
Propamocarb hydrochloride
2,2-diMethoxyadaMantane
Ethyl 1H-imidazole-1-acetate
Sulfuryl chloride
GDF2 ELISA Kit
L-Selectin/CD62L ELISA Kit
Human free chorionic gonadotropin,f-βhCG ELISA Kit
COMT ELISA Kit
ADP ELISA Kit
GABA ELISA Kit
MBP ELISA Kit
PICK1 ELISA Kit
Human ADAMTS5 ELISA Kit
LPS/LOS ELISA Kit
Eluent for the Sterile Test
Membrane-filter Enterococcus Selective Agar
Vinyltrimethoxysilane
3-Furanboronic Acid
5-(2-Chlorophenyl)-1H-tetrazole, 98%
2-Iodobenzoyl chloride, 98%
3,4-DICHLOROPROPIONANILIDE
p-bromo-DL-phenylalanine
Acid Red 215
aminoacetonitrile free base
H-GLY-OIPR HCL
N-N-BUTYL-P-TOLUIDINE
4-AMino-3-Hydroxy-1-Naphthalenesulfonic Acid
N-Boc-D-aspartic acid 4-tert-butyl ester dicyclohexylaMMoniuM salt, 98%
Tetradecanonitrile, 98%
(+)-4,5-Bis[hydroxy(diphenyl)Methyl]-2-Methyl-2-phenyl-1,3-dioxolane
Bicyclohexyl
3-TrifluoroMethyl-2-forMylpyridine
Methyl 3-butenoate
2,5-Difluorobenzaldehyde
3,3′-Diethyloxacarbocyanine iodide
(RS)-1-(3-Fluorophenyl)ethylamine 97%
2,2-Difluoro-1,3-dimethylimidazolidine
Ethyl 5-methyl-1H-pyrazole-3-carboxylate
Piperidine-1-carboximidamide hydroiodide, 97%