SW837[SW-837]细胞系*人结直肠腺癌上皮细胞系开学季大放送 货号:GD-C15120406
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系*将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3,5-Dinitrobenzoyl chloride, 98+%
Cyphenothrin
4-ETHOXYCINNAMIC ACID
1,2-DIIODOETHANE
5-BroMo-2-cyanopyriMidine
BAPTA tetraethyl ester, 98+%
VeMurafenib
6-Isothiocyanato-2,3-dihydro-1,4-benzodioxine
alpha-Toluenethiol, 99%
4-n-Octyloxyphenyl 4-Butylbenzoate [Liquid Crystal]
N,N-Didodecylmethylamine
5-Nitroisatin
Divinyl sulphone
2,4-Dichlorobenzoyl chloride
3-Mercapto-1,2-propanediol
4,5-Dihydro-1,3-benzodioxine-6-aMine
2,5-Di(2-thienyl)-1H-pyrrole
2-BroMo-4-chlorobenzaldehyde
Zinc cyanide, 98%, pure
StearylaMine
2,2'-Oxybis(ethylaMine)
3,4,5-Trimethoxyphenol
Decahydroisoquinoline
N-Benzyloxycarbonyl-L-valine
3-Fluorocinnamic acid
Rhodium(II) acetate dimer
2-Diazo-1-naphthol-4-sulfonate
2-Ethylhexyl acrylate, 98%
5-(4-Fluorophenyl)valeric acid, 97%
3-[Methoxy(Methyl)carbaMoyl]benzeneboronic acid, 97%
Spirodiclofen
4,4-DIFLUORO-L-PROLINAMIDE HYDROCHLORIDE
2,2′-BiphenyldiaMine
OleaMide
NICKEL TELLURIDE
3-(Piperidine-1-carbonyl)phenylboronic acid
2,3-Dihydroxy-6-chloroquinoxaline ,97%
4-Cyclohexylbenzoic Acid
3-N-Cbz-Amino-piperidine
Thiochroman-4-one, 98%
3-Amino-4-chloropyridine
Carbobenzoxyhydrazide
3-(Bromomethyl)biphenyl
2-Chlorobenzamide
6-Chlorobenzo[d]isoxazol-3-ol
N-(tert-Butoxycarbonyl)-p-toluenesulfonaMide
4-Carboxy-2-fluorophenylboronic acid, pinacol ester
Anhydrotetracycline hydrochloride, 'can be used as a secondary standard'
3-Methoxy-2-nitropyridine, 98%
4-AMinostilbene
2-Cyanoethyltriethoxysilane
Isopropyl formate
Pyrazolo[1,5-a]pyridine-3-carboxylicacid, 4,5,6,7-tetrahydro-
3-Bromocinnamaldehyde
ethyl 5-oxo-DL-prolinate
1,3-Dichloro-5,5-dimethylhydantoin
D-Histidine
2-(4-Chlorobenzylthio)ethanol, 98%
(3-CYANOPROPYL)DIMETHYLCHLOROSILANE
2,4-Dichloro-3-ethyl-6-nitrophenol
Iron(II) chloride, anhydrous, 99%
4-Benzyloxy-1H-indole-3-carboxaldehyde, N-BOC protected 98%
1,3-Bis(1-adamantyl)imidazolium chloride, min. 97%
2,4-Dimethylaniline-6-sulfonic acid
4,5,6,7-Tetrahydrothieno[3,2,c] pyridine hydrochloride
(S)-(-)-2-T-BUTYL-2-CARBOXAMIDE
Ethylcyclohexane;
5-BroMobenzo[b]thiophene-3-carbaldehyde
1-BroMo-4-(phenylethynyl)benzene
3-Fluoro-4-(trifluoroMethoxy)phenylboronic acid
Di-n-octyltin oxide
[1,2,4]Triazolo[4,3-a]pyridine-3-thiol, 97%
Oil Orange SS
cis-1,3-CyclohexanediaMine
novobiocin sodium salt
(3R)-3-Amino-3-(4-bromophenyl)propan-1-ol
1-phenyl-3-(trifluoromethyl)pyrazole-4-carboxylic acid
5-bromoisoquinolin-8-amine
RLN ELISA Kit
Mouse S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
Human PCDHβ5 ELISA Kit
Human TPT1 ELISA Kit
IFN-α ELISA Kit
Rat β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA Kit
Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA Kit
Human cholecystokinin octapeptide,CCK-8 ELISA Kit
Human Plasma fibronectin,pFN ELISA Kit
ADH1 ELISA Kit
Aeromonas Medium Base (Rayn)
Wagstsuma Blood Agar Base
ACETYL PHOSPHATE LITHIUM POTASSIUM SALT
SodiuM Hydrogen Difluoride
5,6,7,8-Tetrahydro-2-naphthol
2-Fluoropyridine-4-Boronic Acid
3,4,5-Trimethoxyphenol
1-Propargyl glycerol ether
Methylhesperidine
4-Ketopimelic acid
1,4-Cyclohexanebis(methylamine)
L(+)-2-Aminobutyric acid
2-Chloro-N-(2-fluorobenzyl)aniline, 97%
1,2-Bis(diMethylphosphino)ethane, 97%
2-Chloro-N-(2-fluorobenzyl)benzaMide, 97%
Diphenylvinylphosphine
4,4'-Stilbenedicarboxylic acid
DL-β-AMinoisobutyric acid
(Acetonitrile)[(2-biphenyl)di-tert-butylphosphine]gold(I) hexafluoroantiMonate
2-Hydroxy-4-Methyl-3-nitropyridine
Boron tribroMide
Sodium tert-Butoxide
2-Chloro-6-phenylnicotinonitrile
3-Iodophenylacetonitrile
2-Hydroxy-6-(trifluoromethyl)nicotinic acid