LM8C3H细胞系*小鼠骨肉瘤细胞系开学季大放送 货号:GD-C15119666
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-4-fluoro-5-(trifluoroMethyl)aniline
LanthanuM(III) phosphate hydrate
Benzyl 5-broMopyridin-3-ylcarbaMate
3-ETHOXYBENZOYL CHLORIDE
1,3-BENZOTHIAZOLE-2-CARBONYL CHLORIDE
beta-Methyllevulinic Acid , 98%
Indazole, 96%
Sodium phosphate, tribasic dodecahydrate, for analysis ACS, 98+%
2,5-Dimethoxybenzyl alcohol
2-Fluoro-5-methylpyridine-3-boronic acid pinacol ester
Methyl 3,3-dimethylpent-4-enoate
3,3',5,5'-TETRACHLORODIPHENYL DISULFIDE
ALPHA-PHELLANDRENE
2-Chloro-4-fluoroaniline
Lepidine
DiethyldithiocarbaMic acid
2-Ethylthiazole
2-Chloro-N-ethylacetaMide
2,5-Dihydroxy-1,4-benzenediacetic acid, 99+%
ChroMiuM AtoMic Absorption Standard
1-(Chloro-1-pyrrolidinylMethylene)pyrrolidiniuM Tetrafluoroborate
Glycyrrhizicacidmonoammoniumsalt; 95%
Iron(II) iodide
3-CHLOROPYRIDINE N-OXIDE
3-Nitrobenzyl chloride
1-Butanesulfonyl chloride
GALLIUM(III) SULFATE HYDRATE
Methyl 4-Cyano-3-Fluorobenzoate
(±)-2,2-Difluoro-1-Methylcyclopropanecarboxylic acid, 97%
1-MethyliMidazole-4-carboxylic acid, 95%
NeoMycin sulfate
(−)-Epicatechin gallate
4-(3-BroMophenylsulfonyl)Morpholine
N,N-Diethyl 4-broMobenzaMide
AzidoMethyl Phenyl Sulfide , 97%
Tricobalt tetraoxide
Ethyl 2-aminothiazole-4-carboxylate
4,5-Dimethyl-4H-1,2,4-triazole-3-thiol
N-Methoxy-N-methyl-2-(triphenylphosphoranylidene)acetamide, 98%
4-isopropyl-1,3-thiazole-2-carboxylic acid(SALTDATA: K-salt 0.2H2O 0.16C2H5OH)
CHLORODIBROMOMETHANE
4-tert-Butyl N-[(tert-butoxy)carbonyl]-L-aspartate dicyclohexylamine salt
Thallous malonate
Xylene;Xylol;Xylene Mixture of isoMer
2,6-Difluorophenylboronic acid (contains varying aMounts of Anhydride)
sec-Butyl acetate
Ethyl 2-(5,5-DiMethyl-1,3,2-dioxaborinan-2-yl)benzoate
N-Acetyl 2-broMo-4-nitroaniline
Tris(triMethylsilyl) phosphite, 92%
ENDRIN
2-(tert-ButoxycarbonylaMino)-1-ethanol
methyl cholate
phosphatase alkaline from E. coli
4-Methyl-3-nitrophenol
Hexacosane
CHLORO(DIETHYL)PHOSPHINE
2,4,4-TRIMETHYL-1-PENTENE
5-Fluoro-2-hydroxyphenylboronic acid
1-(4-TRIFLUOROMETHYLPHENYL)
1,4-Bis(chloromethyl)tetrafluorobenzene
5-(tert-Butylsulfonyl)-2-methylpyrimidin-4-amine, 97%
N-Benzyl-2-chloronicotinamide
2-(1-methylpiperazin-2-yl)ethanol(SALTDATA: FREE)
2-(2-PYRIDYL)BENZIMIDAZOLE
Cartap
4-(4-(4-Nitrophenyl)-1-piperazinyl)phenol
2-METHOXYISOPROPYLAMINE
LithiuM Methoxide
2,2-Bis(4-isocyanatophenyl)hexafluoropropane
5-(t-ButylcarbaMoyl)pyridine-3-boronic acid, pinacol ester
alpha-Terpinene, 90%, tech.
Dichlorobis(2-(diphenylphosphino)ethylaMine)rutheniuM(II), 95%
(1S,2R)-2-[N-Benzyl-N-(Mesitylenesulfonyl)aMino]-1-phenylpropyl Propionate [Reagent for anti-selective asyMMetric aldol reaction]
2-(p-Tolyl)ethanol
6-Nitroquinoline
2-Chloro-5-fluorobenzyl alcohol
(-)-2,3-O-isopropylidene-1,1,4,4-tetra-phenyl-D-threitol
4-Chloro-3-(trifluoromethyl)benzyl alcohol
PLAUR/Upar ELISA Kit
Bid ELISA Kit
Human Proinsulin,PI ELISA Kit
Human low-density lipoprotein-receptor-related protein,LRP-6 ELISA Kit
GROb ELISA Kit
IL-9 ELISA Kit
Equine Immunoglobulin E,IgE ELISA Kit
Human M-AChR M2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit
INS ELISA Kit
PⅠNP ELISA Kit
Brucella vaccine medium
Bean Broth Medium
Ethyl 2-Methylquinoline-6-carboxylate, 97%
trans-3-Hexenoic acid, 97%
4-Vinyl-1,3-dioxolan-2-one, 99%
2-Aminoacetophenone hydrochloride
Glycyrrhizicacidmonoammoniumsalt; 95%
3-(Methylthio)butyraldehyde
manganese(2+) bis[(carboxymethyl)azanide]
1,3,5-Trimethoxybenzene
Pyruvic acid
2-Pentafluorophenoxyethanol
3-AMinodibenzofuran
(S)-2-(Boc-aMino)-3-(FMoc-aMino)propionic acid, 98%
2-AMino-4-chloropyriMidine, 98%
ThuliuM(III) nitrate hydrate, REacton|r, 99.9% (REO)
Mercuric sulfate
3-(2-Methyl-3-furylthio)-4-heptanone
5-SULFO-4'-DIETHYLAMINO-2,2'-DIHYDROXYAZOBENZENE
AMberlite? IRP-64 hydrogen forM
SodiuM 2-propylpentanoate
DiethylaMine NONOate diethylaMMoniuM salt
3-Bromobenzotrifluoride
1-ethyl-1H-1,2,4-triazole-5-carbaldehyde(SALTDATA: FREE)
5-Amino-4-(pyridin-2-yl)-1H-pyrazole