K7M2wt细胞系*小鼠骨肉瘤成骨细胞系开学季大放送 货号:GD-C15119656
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-(4-Hydroxyphenyl)ethanol
N-AcetylethanolaMine
(3R,4R)-3,4-Bis(diphenylphosphino)-1-benzylpyrrolidine
VARIAMINE BLUE B SULFATE (=4-AMINO-4'-METHOXYDIPHENYLAMINE SULFATE)[FOR IRON-TITRATION]
2-BROMO-4-NITRO(TRIFLUOROMETHOXY)BENZENE
4-Hydroxy-1-indanone benzoate
2-(4-Methoxyphenyl)quinoline-4-carboxylic acid
(4-Bromophenyl)methanesulfonyl chloride , 97%
2-(Methylthio)pyrimidine-5-carbonitrile ,97%
N-Myristoyl-D-erythro-sphingosine, synthetical, 99+%
(+)-NEOMENTHOL
3-FLUORO-2-FORMYLPYRIDINE
2,3-Dimethoxy-5-methyl-p-benzoquinone
(+)-1,4-DI-O-TOSYL-2,3-O-ISOPROPYLIDENE-D-THREITOL
3,3,3-Trifluoro-2,2-diMethylpropionic acid
2-CyanopyriMidine
Ethyl (S)-(-)-1-Ethyl-2-pyrrolidinecarboxylate
tert-Butyl trans-4-forMylcyclohexylcarbaMate
2-BroMo-1-[2,6-dichloro-4-(trifluoroMethyl)phenyl]ethan-1-one, 95%
CHLOROACETONITRILE
Copper(I) TrifluoroMethanesulfonate Benzene CoMplex
polymyxin B standard solution
germanium tetrafluoride
Sodium Houttuyfonate
Ethyl 3-nitrobenzoate
Manganese triacetate dihydrate
2-Bromo-5-iodopyridine
2,3-Difluoro-6-Nitroaniline
LanthanuM(III) hydroxide, 99.95% (REO)
N,N,N',N'-TetraMethylMethylenediaMine, 99%
Valeric acid
trans-3-Indoleacrylic acid
DIISOBUTYL PHOSPHITE
N,N-Diethylaniline
SODIUM COBALTINITRITE
HexaMethylene diacrylate
4-Chloro-1-methylpiperidine Hydrochloride
3-Methylamino-1,2-propanediol
4-Chloro-5-nitro-o-phenylenediamine, 98%
N-Methylaniline
Hexahydro-1,3,5-tris(hydroxyethyl)-s-triazine
Pilocarpine nitrate
1-IODO-2,3,4,5-TETRAMETHYLBENZENE
QUINACRINE DIHYDROCHLORIDE
4-BroMo-2-Methylaniline
Bis(1,5-cyclooctadiene)nickel(0)
3,4-MethylenedioxycinnaMic Acid
4-(2-Chloro-4-nitrophenyl)Morpholine
2-Chloro-3-fluoropyridine, 97%
Arabic gum
2-Fluorobenzaldehyde
Basil oil french
2-s-Thiuronium ethanesulfonate
N,N-dicyclohexylmethylamine
5-Acetyl-2,2'-Bithiophene
4-[5-(4,4,5,5-TETRAMETHYL-[1,3,2]DIOXABOROLAN-2-YL)-PYRIDIN-2-YL]--1-CARBOXYLIC ACID TERT-BUTYL ESTER
2-BroMo-1-(difluoroMethyl)-4-fluorobenzene
Bis(diMethylaMino)diMethylsilane
5-CHLOROSALICYLANILIDE
(R)-N-FMOC-4-Fluorophenylalanine, 98% ee, 95%
1-Azido-1-deoxy-β-D-galactopyranoside ,97%
4,5,6,7-Tetrahydro-1H-indole-2-carboxylic acid
Fmoc-L-2-indanylglycine
2,4-BIS(TRIFLUOROMETHYL)BENZALDEHYDE
4-N-BUTYLBENZENESULFONAMIDE
1,6-Diiodododecafluorohexane
2,4,6-TRICHLOROPHENOXYACETIC ACID
2-[(4-Chlorophenyl)sulfonyl]ethanethioaMide
2,2-Bis(4-carboxyphenyl)hexafluoropropane
3-Butoxy-5-Methylphenylboronic acid
alpha,alpha'-DibroMo-p-xylene, 97%
Isobutanesulfonyl chloride, 97%
Ibudilast
N-Benzoyl-DL-phenylalanine 2-Naphthyl Ester [for DeterMination of ChyMotrypsin]
1,3-dimethyl-1,3-diphenylurea
6,7-Dihydro-4H-pyrano[4,3-d][1,3]thiazol-2-amine
N-T-boc-ala-pro-nva P-chlorothiobenzyl*ester
N,1-dimethylpyrrolidin-3-amine
TAT ELISA Kit
IκBKβ ELISA Kit
Human IA-2A ELISA Kit
Human hypoxia-inducible factor 1α,HIF-1α ELISA Kit
CDKN2A ELISA Kit
IL-8 ELISA Kit
MMP1 ELISA Kit
Human CHRM4 ELISA Kit
Human Try2 (Trypsinogen II) ELISA Kit
NTXⅠ ELISA Kit
Azospirillum Medium
Martin Agar
Ethyl 3-oxo-3-(4-pyridyl)propionate, 98%
trans-4-(4-n-Pentylcyclohexyl)benzonitrile, 97%
4-Vinylbiphenyl, 97%
2-AMinopyridine-4-carboxylic acid, 96%
polymyxin B standard solution
2,3-DICHLOROBIPHENYL
(S)-7-[[6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2,3-dihydro-5-hydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one
N,N'-Diphenylethanediamide
Isonicotinic acid
N-BENZYLOXYCARBONYL-D-ALANINOL
Lovastatin
2-AMinoadenosine, 97%
Bis(cyclopentadienyl)vanadiuM broMide, 98%
Bis(2-oxo-3-oxazolidinyl)phosphonic chloride
TryptaMine hydrochloride
Chlorfenazole
Phenylacetyl chloride
Reference Material of Hydrocarbon Types in Gasoline by FIA Certified by The Japan PetroleuM Institute, Olefins Content 5vol% level (5Ml*3)
AMbersep 900(OH), ion exchange resin
3,5-Dimethyl-1-phenyl-1H-pyrazole-4-carboxylic acid , 97%
[1,3]Oxazolo[4,5-b]pyridin-2-ol , 97%
Dichlorobis[3-(di-t-butylphosphino)propylamine]ruthenium(II), min. 97%
(S,S)-2,6-Bis(4-isopropyl-2-oxazolin-2-yl)pyridine