Hep-3B细胞,人肝癌细胞系更多价格优惠 货号:GD-C14819251
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
TriethylgerManiuM chloride
9-Benzylcarbazole-3,6-dicarboxaldehyde
HAFNIUM(IV) 2,4-PENTANEDIONATE
3-CHLORO-2-FLUORO-6-(TRIFLUOROMETHYL)BENZALDEHYDE
DIOCTYL ETHER
N,N,N',N'-TETRAETHYLETHYLENEDIAMINE
2-Chloro-5-nitro-N-4-phenylbenzaMide
Mimosine, 99%
4-Benzyloxyindole-3-carboxaldehyde 98%
Sodium 2-Nitroaniline-4-sulfonate
2,3-Dichloro-4-hydroxybenzaldehyde 97%
N,N'-BIS(3-AMINOPROPYL)-1,3-PROPANEDIAMINE
Metribuzin
4,4'-DDE
3-Methyl-5-phenyl-4-isoxazolecarboxylic acid
OctadecyltriMethoxysilane
Di-tert-butyl L-(+)-Tartrate
5-BroMo-2-chloropyridine-4-carbaldehyde
Zinc sulfate heptahydrate, 99.5%, for analysis
SuxaMethoniuM Chloride, Dihydrate, BP
5-Chloro-2-pentanone (stabilized with K2CO3)
2-Methyl-1,1'-biphenyl
Ethyl 3-amino-3-phenylpropanoate
2-(benzoylamino)-3-phenylprop-2-enoic acid
Benzenediazonium, 4-fluoro-, tetrafluoroborate(1-)
4-Fluorobenzeneboronic acid
N-[2-(DIETHYLAMINO)ETHYL]STEARAMIDE
N-MethyloctylaMine
2,3-DiMethylfuran, 99%
2-Iodo-5-nitrotoluene, 98%
4-ChloroMercuribenzoic acid
N,N-DiMethylethylaMine
N-(4-Methoxyphenyl)-4-MethylbenzenaMine
OctaMethylcyclotetrasiloxane
D-CaMphorsulfonic acid
1,3-DIPHENOXYBENZENE
(4-Acetyl-piperazin-1-yl)-acetic acid
6-Chloro-m-anisidine hydrochloride
3-Chloro-6-hydroxy-2-methoxybenzoic acid
3,4-Diaminothiophene dihydrobromide, 99%
TRIPROPYLENE GLYCOL MONOMETHYL ETHER
4,4'-Bis(4-chlorophenyl)sulfonyl-1,1'-biphenyl
4-(Bromoacetyl)pyridine hydrobromide
Kojic acid
2-BroMo-2'-Methoxyacetophenone
L-Carnosine
6-Methoxyflavone
N-Phenyl 3-fluorobenzaMide
2-BroMo-1-[4-(Methylsulfonyl)phenyl]-1-ethanone, 97%
Benzyl 4-hydroxybenzoate
4-(Carboxybutyl)triphenylphosphoniuM BroMide
Methyl 3-(methylthio)propionate
4,7,13,16,21-pentaoxa-1,10-diazabicylco-(8.8.5)tricosane
PRASEODYMIUM SULFATE HYDRATE
Pyrocatechol monoisopropyl ether
2',4',6'-TRIISOPROPYLACETOPHENONE
2-Fluoro-4-forMylphenylboronic acid
4-Nitrobiphenyl-3-carboxylic acid
5-BroMo-2-Methoxyphenylboronic acid
2,3-Dichlorobenzene-1-carbothioamide, 95%
3-Methylthiophene-2-carbonyl chloride
4-Bromo-1-methyl-2-(methylsulfonyl)benzene
3-(4-Chloro-phenyl)-3-oxo-propionic acid ethylester
Hexaammonium molybdate
Diclofop-methyl
4-Ethoxybenzaldehyde
4-Benzyloxyaniline hydrochloride
2-AMino-5-broMo-3-Methylpyridine
1-BroMo-4-fluoronaphthalene
4-Fluoro-2-isopropoxyphenylboronic acid
Diethyl sulfate, 99%
N-tert-Butoxycarbonyl-D-prolinol, 98%
3,4-Diisopropoxy-3-cyclobutene-1,2-dione
SodiuM DL-2-Hydroxybutyrate
Sodium hydroxide
2-Chloro-3-fluoro-5-nitropyridine
ser-phe-leu-leu-arg-asn-pro-asn-asp-*lys-tyr-glu-
fmoc-L-4-hydroxyproline
CFL1 ELISA Kit
Mouse Soluble protein-100,S-100 ELISA Kit
Human PCDHβ13 ELISA Kit
FDFT1 ELISA Kit
TLR4 ELISA Kit
β2-MG ELISA Kit
AMH ELISA Kit
Human Cholic acid,CA ELISA Kit
Human serum/glucocorticoid regulated kinase 2,GSK2 ISA Kit
ACACa ELISA Kit
Modified Phosphate Saline Buffer
Alkaline Agar
SULFANITRAN
Lead(II) fluoride, 99% Min
5,5'-DibroMo-2,2'-bithiophene, 99%
2-Fluoro-4-(4-Morpholinylcarbonyl)benzeneboronic acid, 97%
2-Methyl-1,1'-biphenyl
D-Citrulline
Lectin from Canavalia ensiformis (Con A)
Trimethylene oxide
N-Carbethoxy-4-piperidone
METHYL CALCEIN BLUE
Acid Blue 90
Silver MolybdenuM oxide, 99%
3-Furoic acid, 99%
TriMethylacetaldehyde
Indigotin
2-BroMo-4-Methylthiazole-5-carboxylic acid
Diphenyl sulfone
N'-HYDROXY-3-METHYLBENZENECARBOXIMIDAMIDE
trans-1-Propen-1-yl-boronic acid
4-(2,4-difluorophenyl)-1,3-thiazol-2-amine(SALTDATA: FREE)
1,4-Chrysenequinone
(2R,4R)-4-Methylglutamic acid hydrochloride, 98%
1-(4-tert-Butylphenyl)-2-pyrimidin-4-yl ethanone