BEL-7404细胞,人肝癌细胞系更多价格优惠 货号:GD-C14819131
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Chlorobenzotrifluoride, 98%
3-Methylindene-2-carboxylic acid
3,4-DiMethoxybenzyl isocyanate
1,2,3,4-Tetrachloro-5,5-diMethoxycyclopentadiene
2-NITROBENZHYDRAZIDE
Gal beta(1-3)[Neuc alpha(2-6)]GlcNAc-beta-pNP , 92%
ETHYL 2-METHYL-1,5-DIPHENYLPYRROLE-3-CARBOXYLATE
7-Bromobenzo[b]thiophene, 97%
1,1,3,3,5,5, Hexamethyl diethoxy trisiloxane
Monoerucin
6-methoxyhexanoic acid(SALTDATA: FREE)
4-Bromo-2-fluorobenzaldehyde
METHYLTRIPHENYLPHOSPHONIUM IODIDE
Iridium dioxide
2-BroMo-4'-Methoxyacetophenone
1-Benzothiophene-5-carboxylic acid
Diethyl 2-Ethyl-2-MethylMalonate
C-Undecylcalix[4]resorcinarene Monohydrate
Tris(2-aMinoethyl)aMine, 96%
ScopolaMine HydrobroMide, Trihydrate, USP
Ethyl Chloroglyoxylate
p-Phenylenediacetonitrile
naphthalene-2-sulfonic acid hydrate
trioctylaluminum
5-Bromo-2-fluorobenzotrifluoride
4'-Bromoacetophenone
Dibenzyl azodicarboxylate
2-Chloro-4-Nitrobenzonitrile
GadoliniuM(III) sulfate octahydrate, REacton|r, 99.9% (REO)
1-Boc-3-(ethylaMino)azetidine, 95%
AMMoniuM bisMuth citrate
2-(TriMethylsilyl)pyridine
Dicyclohexyl(2,4,6-triMethylphenyl)phosphine
Phenyldiphenylphosphinite(Diphenylphosphinicacidphenylester)
1-BENZYLPYRROLE-3-CARBOXYLIC ACID ETHYL ESTER
4-FORMYL-TRANS-STILBENE DIETHYL ACETAL
Ethyl azidoacetate
5-Chloro-2-nitrodiphenylamine
1,5-Diaminopentane, 98%
Bis(2-hydroxy-5-chlorophenyl) Sulfide
ANTIMONY (IV) OXIDE
3-Bromophenylboronic acid
4,4'-DIMETHYLOCTAFLUOROBIPHENYL
3,5-DICHLOROSALICYLALDEHYDE
Dodecyl gallate
DiMethyl glutarate
DL-2-Hydroxybutyric Acid (contains PolyMolecular esterification product)
L-4,4'-Biphenylalanine
N-Phenylrhodanine, 97+%
Ethyl nicotinoate
1,2,4-Triazole-3-carboxaMide
Allyldiphenylphosphine
dantrolene sodium
3-Amino-1-propanesulfonic acid
2,4-Dichlorobromobenzene
2-Methoxy-6-(methylamino)pyridine
4-Chloro-3-Methoxyphenylboronic acid
Dichloro[(R)-(+)-2,2′-bis(diphenylphosphino)-1,1′-binaphthyl][(2R)-(?)-1,1-bis(4-Methoxyphenyl)-3-Methyl-1,2-butanediaMine]rutheniuM(II)
FMoc-beta-(1-naphthyl)-D-alanine
5,7-Bis(trifluoromethyl)-3-cyano-2-(methylthio)-pyrazolo[1,5-a]pyrimidine
7-Bromo-4H-1,3-benzodioxine , 95%
Ondansetron
2-Chloro-N-(4-morpholinophenyl)acetamide
NITROSONIUM HEXAFLUOROPHOSPHATE
Pentobarbitone
p-Aminobenzoyl benzamide
Dibenzofuran
1-Methyl-4-piperidineMethanol
1-AMino-2-Methyl-2-propanol
2-Benzyloxy-3-broMo-5-Methylphenylboronic acid
4-Iodobenzyl broMide, 97%
Fluoro-N,N,N',N'-tetraMethylforMaMidiniuM hexafluorophosphate, 99%
L-(+)-Glutamic acid hydrochloride
3,4-Diethoxyphenylacetic Acid
Pentafluorobenzene
4-Chloro-2-fluorophenylboronic acid
1-pyrimidin-2-ylmethanamine
L-tyrosine benzyl ester P-*toluenesulfonate
Dnase-I ELISA Kit
WNT10B ELISA Kit
APOH ELISA Kit
DEFβ130 ELISA Kit
ALT ELISA Kit
ULBP2 ELISA Kit
Chicken follicle-stimulating hormone,FSH ELISA Kit
Human Big ET ELISA Kit
Human Tie-2 ELISA Kit
GMP-140 ELISA Kit
V-KM Medium
Antibiotic test medium Ⅷ No.
(Isopropylthio)benzene, 98%
CalciuM
5-Chloro-2-Benzothiazolinone
6-Chloro-2-Fluoropurine
p-Phenylenediacetonitrile
Procion Blue H-5R
Streptavidin
3-Hydroxytetrahydrofuran
4-(3-FLUOROPHENYL)SEMICARBAZIDE
(3-BOC-AMINOPHENYL)BORONIC ACID
5-BroMo-1,2,3-trifluorobenzene
Methyl 3,4-diMethoxybenzoate, 98+%
TetraMethylaMMoniuM tetrafluoroborate, 97%
(2R,5R)-2,5-Diphenylpyrrolidine
9,9-DiMethylfluoren-2-boronic Acid (contains varying aMounts of Anhydride)
2,4-Diethoxybenzaldehyde
10-CHLORO-3-DECYNE
Pyrazinecarbonitrile
1-BOC-5-Methoxyindole
1-Pentadecene [Standard Material]
4-Methoxycarbonylphenyl isothiocyanate
Bis(4-nitrophenyl)amine
2,6-Dimethylbenzylamine