QGY-7703细胞,人肝癌细胞系更多价格优惠 货号:GD-C14819135
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Methoxy-3-(trifluoroMethyl)benzoyl chloride, 97%
Allyl Methyl sulfide
2-BroMo-5-Methoxyphenylboronic acid
4-ForMyl-2-Methylphenylboronic acid
4-Iodopyridine
2-(TrifluorMethoxy)phenylboronic acid
1-Hexylboronic acid pinacol ester, 98%
1-Bromo-4-(2,2,2-trifluoroethyl)-benzene
2-Amino-5-phenyl-thiophene-3-carboxylic acidmethyl ester
4-(Diphenylamino)phenylboronic Acid (contains varying amounts of Anhydride)
Diallyldiphenylsilane
2-Trifluoromethoxyphenol
3-(Trifluoromethyl)thiophenol
4-Chlorobiphenyl
Cobalt(II) chloride hexahydrate
AMMoniuM trifluoroMethanesulfonate
2,6-DiMethyl-4-hydroxypyridine
1H-Undecafluoropentane
Tris(4-fluorophenyl)phosphine, 99%
SesaMe Oil, NF
4-(2-Methoxyethyl)phenol
phenyl propyl ether
ferric citrate
monoethyl succinate
4-Chloro-3,5-dinitrobenzotrifluoride
Isonicotinic acid
N-BENZYLOXYCARBONYL-D-ALANINOL
Lovastatin
2-AMinoadenosine, 97%
Bis(cyclopentadienyl)vanadiuM broMide, 98%
Bis(2-oxo-3-oxazolidinyl)phosphonic chloride
TryptaMine hydrochloride
Chlorfenazole
Phenylacetyl chloride
Reference Material of Hydrocarbon Types in Gasoline by FIA Certified by The Japan PetroleuM Institute, Olefins Content 5vol% level (5Ml*3)
AMbersep 900(OH), ion exchange resin
3,5-Dimethyl-1-phenyl-1H-pyrazole-4-carboxylic acid , 97%
[1,3]Oxazolo[4,5-b]pyridin-2-ol , 97%
Dichlorobis[3-(di-t-butylphosphino)propylamine]ruthenium(II), min. 97%
(S,S)-2,6-Bis(4-isopropyl-2-oxazolin-2-yl)pyridine
Robenidine hydrochloride
1-Chlorobutane
Dibenzo[b,e]thiepin-11(6H)-one
Tris(2-(2-methoxyethoxy)ethyl)amine
4-[4-(tert-Butyl)-1,3-thiazol-2-yl]benzaldehyde
N-Acetyl-DL-norleucine
4-(Piperazin-1-yl)pyriMidine
3-ChlorobenzaMide, 98%
2-(2-Chlorophenoxy)propionic acid, 98%
L-Valine methyl ester hydrochloride
2-(1,3-Dioxan-2-yl)ethyltriphenylphosphoniuM BroMide
2,4'-Dibromoacetophenone
6-chloro-1,8-naphthyridin-2(1H)-one
2-methyl-5-phenylbenzoxazole
Solvent Blue 45
(3-BENZYLOXY-PHENYL)-ACETIC ACID
BIS(P-TOLYL)PHOSPHINE OXIDE
1-(4-CHLOROPHENYL)-1-CYCLOPROPANECARBOXYLIC ACID
2-IODOBENZOYL CHLORIDE
1-(4-Chlorophenyl)-1-cyclopropanecarboxylic acid, 99%
2-Ethylcrotonaldehyde
Boc-Cys(Fm)-OH
4-Nitrophenyl a-D-maltohexaoside ,98%
1-Methoxy-3-(2-nitrovinyl)benzene
2,4,6-Trimethylaniline
Benzylamine hydrochloride
Ergoloid Mesylates
2,4-DihydroxypyriMidine-5-carboxylic acid
1-AMino-3,6,9,12,15,18-hexaoxahenicosan-21-oic Acid
2-Benzyloxy-6-fluorophenylboronic acid
4-Methoxybenzyl alcohol, 98%
1,7-Dioxaspiro(5.5)undecane, 97%
ISOPRENALINE SULFATE
4-BroMo-2-hydroxybenzonitrile
4-Bromo-2-fluoroaniline
4-Bromothiazole-2-carboxaldehyde
titanocene bis(trifluoromethanesulfonate )
2-Bromo-3-methylbenzoic acid
ApoTf ELISA Kit
MYC ELISA Kit
Human Apolipoprotein E,Apo-E ELISA Kit
Human DEFβ127 ELISA Kit
Gal-3 ELISA Kit
Rat FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
Chicken prolactin,PRL ELISA Kit
Human BP ELISA Kit
Human ANG-R-Tie2 (Angiopoietin Receptor Tie2) ELISA Kit
SAP ELISA Kit
Mycoplasma Agar Medium
Mueller-Hinton Broth
IsopropylMagnesiuM chloride, 1M in MeTHF
GADOLINIUM
5-CHLORO-2,4-DIFLUOROBENZOIC ACID
5-BroMo-2-Fluoropyridine-3-Boronic Acid
phenyl propyl ether
Reactive Brown 2
proteinase from bacillus licheniformis
3-amino-5-fluorobenzotrifluoride
4,4'-Oxydianiline
Benzyl (S)-(-)-1,2,3,4-tetrahydro-3-isoquinolinecarboxylate p-toluenesulfonic acid salt
Biphenyl
6-AMino-3-chloro-1H-indazole, 97%
4-(TrifluoroMethoxy)benzyl chloride, 98%
Methyl 2-broMobutyrate
(+)-2-Methyl-L-serine
FMoc-DL-Nip-OH
EthylenediaMine Monohydrate, 98%
4-AMino-p-terphenyl , 95%
2-Methylbutane
Octyl sulfate, sodium salt, HPLC grade, 99%
3,5-Dimethyl-4-nitroisoxazole ,98%
Potassium (3,5-difluorophenyl)trifluoroborate 96%
Piperidine-1,3-dicarboxylic acid 1-tert-butyl ester 3-ethyl ester