NCI-H295R[H295R]细胞,人肾上腺皮质腺癌细胞系更多价格优惠 货号:GD-C14820366
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3'-Chloroacetophenone, 98+%
(R)-(-)-1-Methyl-3-pyrrolidinol
SodiuM acetate-1-13C
2-HYDROXY-4-METHYLQUINOLINE
Fluoxetine hydrochloride
8-Anilino-1-naphthalenesulfonic acid MagnesiuM salt
Lauryl chloroforMate
(3-Piperidinophenyl)methylamine , 95%
2-(1,3-Benzodioxol-5-yl)-6-chloroimidazo[1,2-b]pyridazine
4,5,6,7-Tetrahydro-3-(trifluoromethyl)-1H-indazole
4-Benzoyl benzylamine hydrochloride
4-Pyridineethanol
3,5-BIS(METHYLSULFONYL)ANILINE
alpha,alpha,alpha-Trifluoro-o-toluoyl chloride
2-Cyano-5-Methylbenzenesulfonyl chloride
FlufenaMic acid
2,4-DiMethyloxazole
N-Methyl 4-broMobenzenesulfonaMide
VancoMycin hydrochloride, >900 MicrograM/Mg
SodiuM Pyrophosphate, Decahydrate, Crystal, Reagent, ACS
Hydroquinidine Hydrochloride
Ethyl Azidoacetate
Brilliant Blue G
8-Fluoroisoquinoline
Alpha,Alpha,Alpha-Trifluoro-p-tolunitrile
Decanoyl chloride
3-Bromobenzenesulfonyl chloride
4-Fluoro-3-Hydroxybenzonitrile
Dicarbonylcyclopentadienylcobalt
AdaMantane-1,3-dicarboxylic acid, 98%
3-AMinobenzenesulfonaMide, tech. 80%
4-PiperidineMethanol
1,1,2,3,3-PENTACHLOROPROPANE
PENTACHLOROTHIOPHENOL
2,4-DIMETHYL-6-NITROPHENOL
1-BroMo-4-(MethoxyMethoxy)benzene
(R)-(+)-(3-Chlorophenyl)oxirane
Ethyldiphenylphosphine, 99%
2,2'-Dihydroxydiphenylmethane
(+/-)-Limonene
(Chloromethyl)dimethylvinylsilane(Vinyl(chloromethyl)dimethylsilane)
Tetraphenylmethane
N-Methyl-L-prolinol
3-FLUORO-L-PHENYLALANINE
PotassiuM pyrosulfate
Benzanilide
3,5-Dichloro-2,4-difluoroaniline
Undecafluorohexanoyl Fluoride
Tris(4-broMophenyl)aMiniuM hexachloroantiMonate, 95%
SeleniuM Sulfide, USP
Diisodecyl 4-Cyclohexene-1,2-dicarboxylate
N-propylaniline
Pigment Red 2
ethambutol dihydrochloride
2-Fluoronicotinic acid
TETRAPROPYLAMMONIUM PERCHLORATE
sec-ButylaMine
5-BroMo-2-benzoxazolinone
2-Methylfuran-3-boronic acid, pinacol ester
5-Fluorouracil
3-Iodoisopropylbenzene
4-Chloro-2,3-dimethylpyridine-N-oxide ,98%
Methyl 4'-nitro[1,1'-biphenyl]-4-carboxylate
2-(4-NITROPHENOXY)ETHANOL
2-Norbornanemethanol
3-CHLORO-2-FLUORONITROBENZENE
CalciuM chloride fused
DI-TERT-BUTYL PHOSPHITE
1-BroMo-2,3-dichlorobenzene
4-MethoxycinnaMonitrile (cis- and trans- Mixture)
Tetrahydrothiophene-3-carboxylic acid 1,1-dioxide
2,6-Difluorobenzaldehyde, 98%
Tungstic acid
3-Pyridylacetic Acid Hydrochloride
ethylenediaminetetraacetic acid*trisodium
Glutaraldehyde sodium bisulfite addition compound
Dextrin
1-chloro-3-ethoxy-2-propanol
PPIL1 ELISA Kit
Smad7 ELISA Kit
Human Progesterone,PROG ELISA Kit
E2/UBCE ELISA Kit
IL13 ELISA Kit
A-GST ELISA Kit
Chicken Myoglobin,MYO/MB ELISA Kit
CH ELISA Kit
Human hemoglobin-advanced glycosylation end products,Hb-AGE ELISA Kit
TAP ELISA Kit
Modified Plate Count Agar
Letheen Broth Base
Ethoxyamine hydrochloride
FluoroMethyl 1,1,1,3,3,3-hexafluoroisopropyl ether, 98+%
5,7-Dichloro-4-Hydroxy-2-(TrifluoroMethyl)Quinoline
2-Fluoro-4-nitrobenzoic acid, 98%
Ethyl Azidoacetate
4-Aminoanisole-3-sulfonic acid
4-nitrophenyl-alpha-L-fucopyranoside
3,5-Dimethylbenzoic acid
SODIUM 4-N-OCTYLBENZENESULFONATE
N-(ISOBUTOXYMETHYL)ACRYLAMIDE
3-Hydroxy-5-Methylisoxazole, 97%
2-(4-Methylphenyl)-4-phenylthiazole, 97%
SodiuM Metasilicate, anhydrous, tech.
tert-Butyl isocyanate
6-Methylnicotinic Acid Methyl Ester
N-Methoxy-N-Methyl 4-borono-2-fluorobenzaMide
2-Chloro-6-trifluoroMethylnicotinic acid
3-CHLORO-4-FLUOROPHENYL ISOCYANATE
1-(4-METHOXY-PHENYL)-2-METHYLAMINO-ETHANOL
Benzyl-propyl-amine
Lead citrate, trihydrate, 96%
7-Methylindole-3-carboxaldehyde 98%
1,2,3,4-Tetrahydrocarbazol-4-one