NR8383细胞,大鼠肺泡巨噬细胞系选用什么培养基 货号:GD-C14519216
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1-Boc-3-piperidone, 97%
3-BroMo-5-(trifluoroMethyl)pyridine
1,2,3,4,5-PentaMethylcyclopentadiene
Gal[246Bn]beta(1-4)Glc[236Bn]-beta-MP , 95%
6,13-Pentacenequinone
Ethyl 5-(4-nitrophenyl)oxazole-4-carboxylate
3-BroMo-4,5-difluoroaniline, 98%
(S)-1-N-Cbz-prolinamide
1-piperidin-2-ylacetone(SALTDATA: 0.15H2O)
4,5-Dichloro-2-fluorobenzoic acid
2,2,2-Trifluoro-N-(7-oxo-6,7-dihydro-5H-indeno-[5,6-d][1,3]dioxol-5-yl)acetamide
N-Acetyl-L-methionine
Calcium 2-ethylhexanoate
ISOPROPYL 3-AMINO-4-METHYL BENZOATE
Tungstic acid
4-BroMo-1-Methyl-3-(trifluoroMethyl)-1H-pyrazole
Di-Mu-chlorobis[5-chloro-2-[(4-chlorophenyl)(hydroxyiMino)Methyl]phenyl]palladiuM(II) DiMer
Methyl 6-broMo-7-chloro-8-Methyl-4-hydroxyquinoline-2-carboxylate
VinyloxytriMethylsilane, 97%
SodiuM Tetrathionate, Dihydrate
Indole-3-acetaMide
Phenylphosphine
3-nitro-4-bromobenzoic acid
3-Phenyl-5-isoxazolone
Ethyl 3-amino-4-fluorobenzoate
Phenazopyridine hydrochloride
4-Hydroxybenzoic acid
2,3-DiMethylpyrazine
Dihydrogen hexachloroplatinate(IV) solution, Pt 20% (cont. Pt)
Phenylhydroquinone diacetate, 98%
(R)-(+)-Glycidol
N-IodosucciniMide
2-BroMo-6-chloro-3-hydroxypyridine
PaMidronate disodiuM salt
5-BroMo-4-o-tolylpyriMidine
LIVER OF SULFUR
Ethyl pyrazolo[1,5-a]pyridine-3-carboxylate , 95%
Potassium Isobutyrate
1-(3,4,5-Trimethoxyphenyl)ethylamine
4-[(Diethylcarbamoyl)amino]benzeneboronic acid, pinacol ester 98%
5-(4-Methylphenyl)-1H-tetrazole
1-BROMO-4-FLUOROBUTANE
2-Cyanoacetamide
DiMethyl yellow;N,N-DiMethyl-4-phenylazoaniline;N,N-DiMethyl-4-(phenylazo)benzenaMine;C.I. Solvent yellow 2;p-DiMethylaMinoazobenzene;Butter yellow;Oil yellow;C.I. 11020
4-(1H-Pyrazol-1-ylMethyl)aniline
N-Hydroxy-5-norbornene-2.3-dicarboxylic iMide
Pentachlorothioanisole
(1S,2S)-(+)-N-(4-Toluenesulfonyl)-1,2-diphenylethylenediaMine, 98%
(2R,3R)-1,4-Di-O-tosyl-2,3-O-isopropylidene-D-threitol, 99+%
LIGNOCERIC ACID METHYL ESTER
Bis(triMethylaluMinuM)-1,4-diazabicyclo[2.2.2]octane Adduct
3-hydroxyadamantane-1-carboxylic acid
(R)-3-amino-1-butanol
4-aminoimidazole-5-carboxamide
oracet blue B
4-Bromobiphenyl
RutheniuM(IV) oxide hydrate
4-METHYL-5-DECANOL
lithiuM octanoate
2,1,3-Benzothiadiazol-4-yl isocyanate , 97%
1-Cyclopropyl- dihydrochloride
4-Nitrophenyl α-L-arabinofuranoside ,98%
1-(2-Bromobenzoyl)pyrrolidine 98%
Benzyl glycinate p-toluenesulfonate
5-Chloro-2-hydroxyaniline
ZINC FORMATE
2-Methylphenylacetic acid
[3-(TrifluoroMethyl)phenyl]Methanethiol
1-BroMo-2-fluoro-4-nitrobenzene
4,5-Dichloro-2-fluorophenylboronic acid, pinacol ester
Cobaltocene, 98%
DL-alpha-Methylbenzyl isothiocyanate, 98%
2,3,5,6-Tetrafluoro-p-xylene
ChloroMethyl p-Tolyl Sulfide
testosterone acetate
(2-fluoro-3-formylphenyl)boronic acid
1-(N,N-dibenzylamino)cyclopropanol
Methyl 5-bromo-2-methylbenzoate
PP ELISA Kit
Slit1 ELISA Kit
Human Progesterone,PROG ELISA Kit
DUB ELISA Kit
IL-1 ELISA Kit
Rat α-Glucosidase,a-Glu ELISA Kit
HSL ELISA Kit
Human choline phosphoglyceride,PC/CPG ELISA Kit
Human HO-1 ELISA Kit
IGF-2 ELISA Kit
Casein peptone agar (G.A)
TGC Medium ,Fluid
3-Oxobutyric Acid Ethyl Ester
Manganese(II) fluoride, Puratronic, 99.99% (Metals basis)
5,6-DiMethoxy-2-(pyridine-4-yl)Methylene-indan-1-one
2'-Fluoro-4'-hydroxyacetophenone, 97%
Phenylphosphine
D-glucal
2-Methoxyethyl cyanoacetate
Coumalic acid
4-Ethylcyclohexanol
4-Hydroxyphenyl Beta-D-Glucopyranoside
5-Methoxy-2-Methylbenzoselenazole, 97%
4-AMino-3,5-dichlorobenzonitrile, 97%
4-ALLYLOXYBENZALDEHYDE
4-(Benzyloxy)-3-Methoxyphenylboronic acid, pinacol ester
1-BroMo-4-tert-butylbenzene
DiacetoxydiMethylsilane
3H-1,2-Benzodithiol-3-one
(R)-N-BOC-4-Cyanophenylalanine, 98% ee, 95%
1-(4-Fluorophenyl)vinylboronic acid, pinacol ester 97%
2-Chloro-4-(trifluoromethyl)-5-thiazolecarboxylic acid phenylmethyl ester
Methyl 3-chloro-5-iodobenzoate