ISK(Ishikawa)细胞,人子宫内膜癌细胞系多久传代 货号:GD-C14120532
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3'-BroMobiphenyl-3-carboxylic acid, 95%
Karbutylate
OctyltriMethylaMMoniuM chloride
METHYL N-MORPHOLINOACETATE
4'-Chloroacetophenone
2,4,5-TriMethylbenzoic acid
2,6-Difluoroanisole 98%
6-Bromo-1-hexene
acth(1-10)
3,5-Dichlorobenzoylacetonitrile
4-Pyrrolidin-3-yl morpholine
METHYL 1-METHYLPYRROLE-2-CARBOXYLATE
1H,1H-HEPTAFLUOROBUTYL ACRYLATE
3-Amino-4-bromopyrazole
2-DiMethylaMinoethyl chloride hydrochloride
1-[4-(1H-Indol-3-yl)piperidino]ethan-1-one
2,2-DiMethylhexanenitrile
N-Propyl 2-broMo-5-fluorobenzaMide
Methyl 2-chloro-4,4-diMethyl-3-oxopentanoate, 90%
TRICHLOROACETIC ACID
1,4-Dibutoxybenzene
Biphenyl dimethyl dicarboxylate
dichloro[tris(2-methylphenyl)]-lambda~5~-bismuthane
[[4-[[4-(phenylimino)-2,5-cyclohexadien-1-ylidene][4-[(sulphophenyl)amino]phenyl]methyl]phenyl]amino]benzenesulphonic acid
trans-4-hydroxy-3-methoxycinnamic acid
3-(3,4,5-TRIMETHOXYPHENYL)PROPIONIC ACID
TRANS-PERMETHRIN
1-Benzyl-4-Methyl Hydrochloride
1-Fluoro-4-hydroxy-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate), ca 45-50% w/w on aluMina
3-BroMo-2-chlorobenzotrifluoride, 97%
3′-(TrifluoroMethyl)acetophenone
3-AMinopyrazole-4-carbonitrile
2-CHLORO-5-FLUORONITROBENZENE
N-Methyl-1-phenylpropylaMine
4-BroMo-1H-pyrrole-2-carboxaMide
4-BroMobiphenyl-4-yl triflate
4,5-Dimethyl-1,3-thiazol-2-amine hydrobromide , 97%
4-Aminobenzamide
4-methyl-3-phenylisoxazol-5-amine(SALTDATA: FREE)
5-Bromobenzo[b]thiophene-2-boronic acid
GUANFACINE HCL
2,4,5-TRICHLOROTHIOPHENOL
Boc-L-Pyroglutamic acid methyl ester
3,4-Dichlorotoluene
4-(2-Thienyl)benzaldehyde
4-Ethylbiphenyl
Octatriacontane
1-Cyclopropyl-1,3-butanedione
4-(1,2,3-Thiadiazol-4-yl)benzoyl chloride, 97%
2-Octanol
2-AMino-5-chloro-2'-fluorobenzophenone
4-(4-Fluorobenzoyl)piperidine hydrochloride
Iridium(III) acetate
methyl (R)-lactate
2-Bromo-4-methylbenzoic acid
Methyl 3-methylthiopropionate
4-AMino-3-(4-chlorophenyl)butyric Acid, Baclofen
TRANS-1,2-DICYANO-1,2-BIS(2,4,5-TRIMETHYL-3-THIENYL)ETHENE
THORIN
4,5,6,7-Tetrafluoroindole-3-carboxaldehyde
1,3-Cyclopentanediol, mixture of cis and trans, 95%
t-Butyldicyclohexylphosphine, min. 95%
Phenyl 4,6-O-Benzylidene-1-thio-beta-D-glucopyranoside
2-Chloro-6-methylpyridine-4-boronic acid, pinacol ester
ALLYLTRIPHENYLTIN
1,2,2,3-TETRACHLOROPROPANE
5-FLUORO-2-METHOXYBENZALDEHYDE
Cyclohexyl broMide
1H,1H-Perfluoro-1-octanol
Dexrazoxane
4-Chloroindole-3-carbaldehyde
PhenacyltriphenylphosphoniuM broMide, 96%
Ursodiol, USP
1,1-Diethylurea
N-methyl-2,2,2-trifluoroacetamide
Dimethyldiselenide
9H-Carbazole-ethanol
4-Iodoaniline
ADM ELISA Kit
PAL ELISA Kit
HP-lgM ELISA Kit
PMN ELISA Kit
P ELISA Kit
γ IFN-Ab ELISA Kit
MT ELISA Kit
Human PKCδ1 (Protein Kinase C Delta ) ELISA Kit
Human PAFAH2 ELISA Kit
MDA ELISA Kit
Phosphate solution(1M,pH6.0)
Dulbecco’s Phosphate Buffered Saline(D-PBS)
Vinylcyclohexane, 97%
(-)-Fenchone, 98+%
Methyl 4-n-octyloxybenzoate, 98%
2-Iodophenylacetonitrile, 96%
Biphenyl dimethyl dicarboxylate
BIS(TRIPHENYLPHOSPHINE)NICKEL(II) BROMIDE
3-Ferrocenylpropionic acid
Carnitine D-form
2,4-DICHLOROBENZHYDRAZIDE
2,2'-(1,2-Ethenediyldi-4,1-phenylene)bisbenzoxazole
3,4-Diethoxy-3-Cyclobutene-1,2-Dione
3-BroMo-5-iodobenzoic acid, 97%
6-ChloroiMidazo[1,2-a]pyridine, 97%
Methyl decanoate
2-Fluoro-5-nitroaniline
3-Chloro-2-nitrobenzyl alcohol
8-ANILINO-1-NAPHTHALENESULFONIC ACID AMMONIUM SALT HYDRATE, 97
METHYLMAGNESIUM IODIDE
DICHLORO-[1,3-BIS(DIISOPROPYLPHENYL)-2-IMIDAZOLIDINYLIDENE]-(3-CHLOROPYRIDYL)PALLADIUM(II)
Benzyltrimethylammonium Hydroxide (10% in Water)
2,4,6-Triphenylnitrobenzene
4-(1H-Tetrazol-5-ylcarbamoyl)benzeneboronic acid hydrochloride 97%
M344