HEC-1B细胞,人子宫内膜腺癌细胞系多久传代 货号:GD-C14120523
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Methyl-5-(trifluoroMethoxy)benzoic acid, 97%
Hyaluronic acid sodiuM salt froM rooster coMb
TriMorpholinophosphine oxide
AMberlite XAD-1180
3-Fluoropicolinic acid
4-NITROBENZO-15-CROWN-5
2-(Trifluoromethyl)-imidazo[1,2-a]pyrazine
n-Triacontyldimethylchlorosilanetech grade
(4-Methoxy-pyridin-2-yl)-carbamic acid tert-butyl ester
Thiazolidine
2,4'-Dihydroxydiphenyl Sulfone
Trimethylolpropane trimethacrylate
Calcium nitrite
4,6-Dichloro-5-pyrimidinecarbaldehyde
2-(5-Chloro-3-Methylbenzo[b]thiophen-2-yl)acetic acid
4-ChlorobenzylaMine
Diethyl 2,2'-Thiodiglycolate
4-BroMo-3-(trifluoroMethyl)benzaldehyde
2-FluorobenzylaMine, 96%
Trazodone Hydrochloride, USP
N,N,N'',N''-TetrabutyldiethylenetriaMine
Tricyclo(3.3.1.13,7)decane-1-carboxylic acid, methyl ester
2,4,6,8-Tetrahydroxy-Pyrimido-(5,4D)Pyrimidine
calcium diundec-10-enoate
Rosmarinic acid
2-Amino-4-methoxy-6-methylpyrimidine
Flufenoxuron
3-Iodobenzonitrile
O-Benzylphospho-N-FMoc-L-serine, 95%
N-Benzyl-4-fluorobenzylaMine, 97%
2-NitrobenzenesulfonaMide
N,N-DiMethylbutylaMine
3,5-Difluoro-2-nitrophenylboronic acid
N-Methylaniline
1-ACETOXY-1,3-BUTADIENE
N,N-DiethylsalicylaMide
6-Fluoronicotinaldehyde
2-Chlorobenzoylacetonitrile
D-Glutamine methyl ester hydrochloride
N-Carbobenzoxy-L-isoleucine Dicyclohexylammonium Salt
Lofexidine hydrochloride
5-Azacytidine
Cyclobutanecarbonyl chloride
LAURIC ACID SODIUM SALT
2,3-DichlorobenzylaMine
Dibenzo(a,h)anthracene D14 solution
2-Nonene (cis- and trans- Mixture)
IMidocarb
(-)-8-PhenylMenthol, 97%
N-PHENYLAZA-15-CROWN 5-ETHER
1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one
m-Methyl red
1-(methylsulphonyl)imidazolidin-2-one
2-Chloro-3-methylbenzoic acid
o-Sulfobenzoic acid monoammonium salt
DL-2-Octanol
DIMETHYL 2-(THIOPHEN-2-YLMETHYL)MALONATE
Hexyl alcohol
P-TOLUENESULFONYL-N,N-DIETHYLAMIDE
4-Hydroxy-1-methylbicyclo[2.2.2]octan-2-one
4'-Amino-3'-(trifluoromethyl)acetanilide
4-Ethoxy-3-nitropyridine hydrochloride
8-Aminoquinoline N-Oxide
FMOC-D-2-PYRIDYLALANINE
1-CHLORO-3-METHYLBUTANE
1,2,3,4-TETRAHYDRO-9-ACRIDINAMINE
2-Heptanol;MethylaMyl carbinol;sec-Heptyl alcohol;2-Hydroxyheptane;Methyl pentyl carbinol
1,2-Difluoro-4-iodobenzene
1-Ethylpiperidine
Nepafenac
Methyl 2-aMinobenzo[d]thiazole-7-carboxylate
3H-1,2-Benzodithiol-3-one 1,1-dioxide, 98%
Methyl (triphenylphosphoranylidene)acetate
Ethyl Nonanoate
2-Bromo-3-methyl-5-nitropyridine
dichloroisocyanuric acid sodium salt dihydrate
Dimethoxymethylvinylsilane
Trimethylsilyl cyanide
GDF1 ELISA Kit
CACNα1D ELISA Kit
Human Helicobacter pylori IgG ,Hp-IgG ELISA Kit
Human MRP1 (Multidrug Resistance Associated Protein 1) ELISA Kit
Monkey apoprotein B100,apo-B100 ELISA Kit
γSAP ELISA Kit
CA2 ELISA Kit
Human PKCβ1 ELISA Kit
PAF ELISA Kit
FABP ELISA Kit
Dulbecco’s Phosphate Buffered Saline(D-PBS)
Cary-Blair Transport Medium
Trichlorovinylsilane
Furan-2-thiocarboxaMide, 97%
5-(2-CHLOROPHENYL)-2-FUROIC ACID
2-Iodobenzoic acid, 98+%
Tricyclo(3.3.1.13,7)decane-1-carboxylic acid, methyl ester
Benzo-18-crown-6
Acid Yellow 116
Tert-butyl nitrite
2,5-Dimethylphenylacetonitrile
4-Chloro-2-fluorophenol
Diisobutyl adipate
AMMoniuM diaMMinetetrathiocyanatochroMate(III) Monohydrate, ACS, 93.0% Min
(+)-Fenchone, 98%
Tetrahydrofuran-3-ylaMine hydrochloride
Tributylphosphine solution
Methyl 1H-indazole-6-carboxylate
(S)-1-[(RP)-2-(Diphenylphosphino)ferrocenyl]ethyldi(3,5-xylyl)phosphine
3-(2-Naphthyl)-D-alanine
1,2-Dichloropropane
2,3-Dichlorophenyl isocyanate
(4-sec-butylphenyl)boronic acid(SALTDATA: FREE)
Ethyl 2-(Hydroxymethyl)acrylate (stabilized with HQ)
5-Nitro-2-(3-piperidinylmethoxy)pyridine hydrochloride