NCI-H23[H23]细胞系;人肺癌细胞系降价大促销 货号:GD-C13720475
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4'-Hydroxyacetophenone, 99%
3-Methylbenzenethiol
4-AMino-3-fluoropyridine
1,2,4-Benzenetricarboxylic anhydride
2,3,4,5,6-PENTAFLUOROPHENOXYACETYL CHLORIDE
N-Octyl pyrrolidone
1-BUTYL-3-METHYLIMIDAZOLIUM METHANESULFONATE
Isoamyl Isobutyrate
3,4-Methylenedioxyphenethylamine hydrochloride
5-Oxo-pyrrolidine-3-carboxylic acid methyl ester
(3S-cis)-(+)-7a-Methyl-3-phenyltetrahydropyrrolo-[2,1-b]oxazol-5(6H)-one, 97%
Tamsulosin hydrochloride
Dimethyl fumarate
Cupric carbonate basic
tert-Butyl 4-[4-(ethoxycarbonyl)-1,3-thiazol-2-yl]tetrahydro-1(2H)-pyridinecarboxylate
2,6-Dichloro-4-Methylnicotinic acid
2,5-DibroMoselenophene
5-BroMo-1-(4-Methoxybenzyl)pyrrole-2-carbaldehyde
2-Hydroxy-4-Methylbenzaldehyde, 97%
TetrabutylaMMoniuM Hydroxide, 55 Percent (w/w) Aqueous Solution
DisodiuM 4-AMino-4'-nitrostilbene-2,2'-sulfonate
3-Amino-1-adamantanol
6-amino-1,3-dimethyluracil
Tungsten(VI) oxide
2,6,6-trimethylcyclohepta-2,4-dien-1-one
5-Hydroxymethylpyrrolidin-2-one
(2-HYDROXYETHYL)TRIPHENYLPHOSPHONIUM BROMIDE
6-Chloro-2,4-di(4-Morpholinyl)pyriMidine, 97%
2,2-Di-n-propylacetaMide, 97%
1-TriMethylsilyl-1,4-pentadiyne, 98%
4-(TrifluoroMethoxy)aniline
2,3,4,6-TETRA-O-ACETYL-BETA-D-GLUCOPYRANOSYL ISOTHIOCYANATE
Ethyl 3-broMo-2-(broMoMethyl)propionate
N-Propyl 3-broMo-5-nitrobenzaMide
2,4-Dichloro-6-MethylpyriMidine
(1-Chloroethyl)triMethylsilane
cis-1,2-Cyclohexanediamine
4-(tert-Butoxycarbonylamino)benzylamine
Imidazo[1,2-a]pyrazine-2-carboxylic acid
2-Hydroxy-2-methylpropiophenone
3-Bromo-2,6-dimethylpyridine
4,5-Dicyano-2-aminoimidazole
Antimonous hydride
2-[3-(2H-Benzotriazol-2-yl)-4-hydroxyphenyl]ethyl methacrylate
4-Fluorobenzyl alcohol
Nalpha-(tert-Butoxycarbonyl)-L-arginine 4-Nitroanilide Hydrochloride
Ethyl 6-MethyliMidazo[1,2-a]pyridine-2-carboxylate
alpha-MethylferroceneMethanol, 97%
1-Benzyl-4-cyano-4-hydroxypiperidine, 98%
LithiuM Dodecyl Sulfate [for BiocheMical Research]
Benzenesulfonic Acid Anhydrous
4,4'-Dimethylbiphenyl
5-Bromo-2,3-difluoroanisole
3-Pyridazinylmethylamine
oxiconazole nitrate
3-METHYL-3-HEXANOL
1,3,5-Benzenetricarboxylic acid chloride
8-Fluoroquinoline-7-boronic acid
3-(MethylaMino)propionaMide
3',4'-(Didecyloxy)benzaldehyde, 96%
3-Bromo-5-fluorobenzyl bromide 98%
2-Formylfuran-4-boronic acid pinacol ester
Cyclohexylmethyltriphenylphosphonium iodide
4-Chlorobenzenesulfonic acid
(S)-4-(4'-Nitrobenzyl)-1,3-oxazolidine-2-one
(R)-(-)-Epichlorohydrin
3-Fluoro-4-methylbenzoic acid
2,1,3-Benzothiadiazole-4-carbaldehyde
1-Chloro-2,5-diethoxybenzene
4-Propoxy-2,3,5,6-tetrafluorophenylboronic acid
Hydrogen broMide, pure, 33 wt.% solution in glacial acid, AcroSeal
1-Acetyl-3-indolecarboxaldehyde, 98%
Miconazole Nitrate
2-Methoxybutyl Acetate
o-Fluoro Thiophenol
Phenylacetaldehyde dimethyl acetal;1,1-Dimethoxy-2-phenylethane
3-Bromo-7,12-diphenylbenzo[k]fluoranthene
(2a,6a,8a,9ab)-Hexahydro-8-hydroxy-2,6-methano-2H-quinolizin-3(4H)-one
OX-B ELISA Kit
P450scc ELISA Kit
Human OSCP1 ELISA Kit
Human DDH2 ELISA Kit
LEP ELISA Kit
BLI ELISA Kit
Chicken Heat Shock Protein 20,HSP-20 ELISA Kit
Human PROZ ELISA Kit
Human TM ELISA Kit
APOB ELISA Kit
Oxford Agar Base
Nitrate peptone water Medium
CalciuM Acetylacetonate
SodiuM fluorosilicate
5-(4-Morpholinyl)-2-nitrophenol, 97%
Ethyl 2-butynoate
3-Amino-1-adamantanol
TETRAHYDROXYDIBORON
Platinum(IV) oxide
3-Hydroxy-4-methoxycinnamic acid
COAL TAR
3-Chloropropionic Acid Methyl Ester
Tetrahydrothiophene, 98%
4-n-Heptyloxybenzaldehyde, 97%
Methyl 4-(2,2,2-triMethylacetaMido)nicotinate
6-Methoxysalicylic Acid
Tea tree oil
Butyryl chloride
Methyl 4-broMopicolinate
4,4'-DIMETHOXYBENZHYDROL
2-Methylthiazole
1-Methyl-1H-indazole-3-carbonyl chloride, 97%
(S)-1-Benzyl-pyrrolidin-3-ol
meso-2,3-Dimethylsuccinic Acid
5-Fluoro-2-methoxybenzyl alcohol