CCC-SMC-1细胞系;兔主动脉平滑肌细胞系专注培养平台 货号:GD-C13619206
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-AMinobenzoic acid, 98%
Nicotinoyl chloride hydrochloride
5-Benzyloxy-1-BOC-indole-2-boronic acid
N-[3-(BOC-AMINO)PROPYL]GLYCINE ETHYL ESTER
Citral diethyl acetal
2-Methoxycarbonyl-3-Methylphenylboronic acid
1,1'-OXALYLDIIMIDAZOLE
Ethyl 4-pyridylacetate, 97%
(S)-(-)-1-Ethyl-2-pyrrolidinecarboxamide
4-Bromo-2-(pyrrolidin-1-ylsulphonyl)anisole 95%
3-Chlorobenzal Bromide
2-Thienylmethanethiol
L-Cysteine hydrochloride anhydrous
1-(3-CHLOROPHENYL)BIGUANIDE HYDROCHLORIDE
2,5-Hexanediol
2-(MethylaMino)ethanol
2,6-DichlorodiphenylaMine
2-AMino-5-forMylthiazole
Vinyl acetate, 99+%, stabilized
SodiuM Starch Glycolate, pH 5.5-7.5, Type A, NF
LuMinol [CheMiluMinescence Reagent]
p-Hydroxy Thiophenol
L(+)-Glutamic acid monosodium salt monohydrate
5-Chloro-2-methylindole
benzoyl fluoride
4-(2,6-DIMETHYLPHENYL)-3-THIOSEMICARBAZIDE
Ethanol
Tetra-n-butylaMMoniuM acetate, 1.0M aq. soln.
Thiophene-2-carboxylic anhydride, 95%
Copper based Methanol reforMing catalyst, HiFUEL^t R120
4-BroMo-3,5-diMethoxybenzoic acid
2-AMINO-4-NITROPHENOL SODIUM SALT
Cyanoethyl Cellulose
Pentacene
2,4-DiMethoxy-1-fluorobenzene
Anthrarobin
Tetramethylammonium hexafluorophosphate 99%
2-Nitroaniline
4-Amino-1,8-naphthalimide, 98%
Metrizoic Acid
CIS-1,2-CYCLOHEXANEDICARBOXYLIC ANHYDRIDE
3,3-Dimethyl-D(-)-cysteine
Phenyl-(2-pyridyl)acetamide
Eleutheroside E
1-AdaMantanaMine
cis-4-tert-Butylcyclohexanecarboxylic Acid
3-Fluoro-4-cyanopyridine
Cellulose Microcrystalline, average particle size 50áM
1-(2-Phenyl-1,3-thiazol-4-yl)ethan-1-one, 95%
Fexofenadine Hydrochloride
sec-Butyl Butyrate
1-(Diphenylmethyl)
1-(5-bromothiophen-2-yl)methanamine
3-Tetradecylthiophene
diethyl (2R,3R)-oxirane-2,3-dicarboxylate
2-Ethoxynaphthalene
(4-Pyridylthio)acetic acid
DiMethyl 3,3'-thiodipropanoate
CORONENE
2-Methoxybenzylamine, 98%
1-methyl-3-phenyl-1H-pyrazol-5-amine(SALTDATA: FREE)
Perylene, 99+%
Perfluorobut-2-ene (FC-1318my) 97%
2,4,5-TRIFLUOROBENZYL CHLORIDE
19-Norandrost-5(10)-ene-3,17-dione
2-CHLORO-6-FLUOROBENZAMIDE
DiisopropylaMine;N-(1-Methylethyl)-2-propanaMine; DIPA;
(4-Chlorophenyl)(4-piperidyl)Methanone hydrochloride
1-BroMo-2,4-dichlorobenzene
Isopropenyloxytris(triMethylsilyl)silane
1-(4-BroMophenyl)-4-(ethylsulfonyl)
4-Phenoxyaniline, 97%
BASIC BLUE 24
1,2-Naphthoquinone-2-diazido-4-sulfonyl Chloride
Kasugamycin
ketoprofen
z-D-Arg
4-amino-1,1'-azobenzene-3,4'-disulfonic acid
FetuinA ELISA Kit
Mouse Smad4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Human Pg ELISA Kit
Human UBE1L ELISA Kit
IL12B ELISA Kit
α-GST ELISA Kit
MYO/MB ELISA Kit
Human SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit
Hb-AGE ELISA Kit
INS ELISA Kit
Yeast Extract Agar
Eugon Broth
6-ETHOXY-2-BENZOTHIAZOLESULFONAMIDE
EUROPIUM FLUORIDE
5,6-Diphenyl-3-(2-pyridyl)-1,2 4-triazine, 99%
2-Fluoro-4-nitrobenzaldehyde
p-Hydroxy Thiophenol
Cyclohexyl chloroformate
Glybenzcyclamide
Isomaltose
Cobalt acetate
5-Chloroindole-2-Carboxylic Acid
2,2,6,6-TetraMethyl-4-piperidone Monohydrate, 99%
1-BroMo-2-Methylnaphthalene, tech. 85%
6-Benzoylhexanoic acid, 94%
p-Tolyl isocyanate
3,4-DIHYDROXYBENZYLAMINE HYDROBROMIDE
METHYL 2-ISOTHIOCYANATOBENZOATE
(S)-(+)-4-NITRO-7-(3-AMINOPYRROLIDIN-1-YL)-2,1,3-BENZOXADIAZOLE
THIOPHENE-3-CARBOXALDEHYDE DIETHYL ACETAL
4-HYDROXYBENZOIC ACID ALLYL ESTER
2-Amino-6-iodo-7H-purine
N-Acetyl-L-cysteine
Benzoyl-beta-alanine
Trihexylphosphine