CCC-SMC-2细胞系;兔主动脉平滑肌细胞系专注培养平台 货号:GD-C13619204
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3'-AMinoacetophenone, 97%
Iron acetylacetonate
2,2-DIPHENYLPROPIONIC ACID
2,3-DIFLUORO-4-METHYLBENZYL BROMIDE
Ethyl 2-acetylcaprylate
2,4,6-TRI-TERT-BUTYLNITROBENZENE
COMANIC ACID
L-Arginine methyl ester dihydrochloride, 98%
Undecyl Ether
Methyl 3-(cyclopropylamino)-2-(2,4-dichloro-5-fluorobenzoyl)acrylate
3-Hydroxyaspartic Acid
Ethyl 4-amino-2-(ethylthio)-5-pyrimidinecarboxylate
4-BROMO-2-(TRIFLUOROMETHYL)PHENYL ISOTHIOCYANATE
N-Cbz-N-methyl-L-leucine
TetrapropylaMMoniuM chloride
5-(4-Chlorophenyl)furfural
Diallyl 1,4-Cyclohexanedicarboxylate (cis- and trans- Mixture)
2-AMino-4-broMobenzaldehyde
Vanillin, 99%, pure
SodiuM Pyruvate, Reagent
1,1,3-Trichloroacetone
1-Chloro-4-iodobenzene
Brilliant Blue R
Indole-5-methanol
alpha,alpha,alpha-Trifluoro-p-tolualdehyde
3-METHYL-5-HEXEN-3-OL
4-Cyanopyridinium-1-olate
3-Fluoro-2-Methylpyridine
8-Chloro-4-hydroxy-2-(trifluoroMethyl)quinoline, 97%
N,N'-DiethyloxaMide, 98%
4-(5-Methyl-1,2,4-oxadiazol-3-yl)benzoic Acid
3-Acetylpyridine
1,6-DIAMINOHEXANE-N,N,N',N'-TETRAACETIC ACID
PENTACHLOROTHIOANISOLE
2-Fluorophenylboronic acid, pinacol ester
3,4-O-ISOPROPYLIDENE-D-MANNITOL
3-Amino-5-methoxy-pyridine
1-(2,5-Dichlorophenyl)-2-thiourea
Acid Red 60
3-pyridin-3-yl-1H-pyrazol-5-amine(SALTDATA: 2.1HCl 0.2H2O)
4-Carboxyphenylboronic acid
Fmoc-Cys(tBu)-OH
Eprodisate
2,5-Dihydrofuran
Phosphotungstic acid hydrate
PotassiuM cyanate
ThauMatin
2,4,4,6-TetrabroMo-2,5-cyclohexadien-1-one, 95%
BariuM broMide, 99.999%, (trace Metal basis), extra pure, anhydrous
Z-LYS-ONP HCL
tert-Butyl 3-Oxo-1-carboxylate
N-[(4-chlorophenyl)methyl]-2,2,2-trifluoro-Acetamide
N-Fmoc-N-methyl-O-benzyl-L-serine
3-Bromo-4-chloropyridine
1,4-Cyclohexanedimethanol bis(3,4-epoxycyclohexanecarboxylate)
2-Diisopropylaminoethyl chloride hydrochloride
LithiuM L-aziridine-2-carboxylate
4-Methoxypyridine-3-boronic acid, HCl
6-BroMotetrazolo[1,5-a]pyridine
4-Benzylbiphenyl
(3S)-(-)-3-(Ethylamino)pyrrolidine
Methyl indole-2-carboxylate ,97%
N-Boc-1,2,3,4-tetrahydroisoquinoline-6-carboxylicacid
2-Cyano-phenothiazine
Gestodene
2,6-DIPHENYLPYRIDINE
DiethylaMine;
Methyl Orange-xylene cyanol solution in water
1-BroMo-2,3-difluorobenzene
Isoguvacine Hydrochloride
4-(4-BroMophenyl)-N,N-diMethyl-1-sulfonaMide
2-Chloro-5-pyridineboronic acid, 97%
TRIOXSALEN
3,4-Difluorobenzonitrile
1-Cyclohexenylacetonitrile
Chloromethyl chloroformate
Methyl 2-oxopentanoate
sulfuryl fluoride
PPI ELISA Kit
Mouse Smad4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Human PROG ELISA Kit
Human E2/ubiquitin-conjugating enzyme,E2/UBCE ELISA Kit
Chicken IL-12 p40 (Interleukin 12 p40) ELISA Kit
Rat α-glutathione S-transferases,α-GST ELISA Kit
MYO ELISA Kit
Human CH25H ELISA Kit
Human hemoglobin-advanced glycosylation end products,Hb-AGE ELISA Kit
IAPP ELISA Kit
Modified Plate Count Agar
Letheen Agar Base, Modified
AntiMony(III) ethoxide, 99.9% (Metals basis)
EuropiuM(III) fluoride, anhydrous, 99.5% (REO)
5,7-Dichloro-1-indanone, 97%
2-Fluoro-4-nitrobenzoic acid
1-Chloro-4-iodobenzene
4,5,6,7-tetrachlorofluorescein
Isopropyl laurate
3-Fluoro-5-nitrotoluene
1-Benzyl-4-hydroxypiperidine
Carrot Seed Oil
Undecylenic Acid
Bis(cyclopentadienyl)vanadiuM, subliMed, 95%
Nitrobenzene-d{5}, 99% (Isotopic)
(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt
Nα-Benzoyl-DL-arginine β-naphthylaMide hydrochloride
(R)-(+)-Trityl glycidyl ether
PENTAMETHYLENEBIS(TRIPHENYLPHOSPHONIUM BROMIDE)
3-(TriMethylsilyl)propynoic acid
4-(2-CHLOROETHYL)BENZOIC ACID
6-(2-Hydroxyethylamino)-1,3-dimethyl-1H-pyrimidine-2,4-dione ,98%
N,N-Dipropylaniline
4-Chloro-3,5-difluorobromobenzene 97%
(S)-(-)-2,2'-Bis(di-p-tolylphosphino)-6,6'-dimethoxy-1,1'-biphenyl, min. 97%