MUS-M1细胞系;小鼠小肠平滑肌细胞系专注培养平台 货号:GD-C13619165
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-(Methylthio)benzeneboronic acid, 97%
Pyrifenox
2-(1-Methylpropyl)-thiazole
PR(TMHD)3
3-(ChloroMethyl)pentane
4-BroMo-2-fluorotoluene
(3R)-(+)-3-(ETHYLAMINO)PYRROLIDINE
7-Acetoxy-4-methylcoumarin
1H,1H,2H-Perfluorocyclopentane
Ethyl Phenyl Sulfide
5-[4-(tert-Butyl)phenyl]-1H-1,2,4-triazole-3-thiol
ISOCYANOBENZENE
2-FLUORO-DL-PHENYLALANINE
FMOC-SUBEROL
4-Hydroxy-2-(trifluoroMethyl)pyriMidine
18-Crown 6-Ether
4,6-DiMethyl-2-(Methylsulfonyl)pyriMidine
Voriconazole
TryptaMine, 98%
SodiuM Borohydride, Crystalline Powder, Reagent, ACS
5-(4-Chlorobutyl)hydantoin
Ethyl 2-mercaptoacetate
2,2-dimethyl-propanedioic acid dimethyl ester
2-acetylpyrrole
4-Fluoro-2-methoxybenzoic acid
2'-BROMO-4'-FLUOROACETOPHENONE
4-ETHYNYLANILINE
Guanine Hydrochloride
LithiuM octanoate, 99%
PalMitic acid, 95%
L-Isoleucine allyl ester p-toluenesulfonate salt
Bis(diethylaMino)phosphorochloridate
TRANS-3,4-DIMETHYL-2-PENTENE
PHENACETURIC ACID
5-(3-BroMophenyl)-1H-pyrazole
5-BROMO-2-(TERT-BUTOXY)PYRIMIDINE
Cholesterol Formate
Doxycycline monohydrate
N-(3-Aminopropyl)glycine Dihydrochloride
tert-butyl (5-bromo-2-pyrimidinyl)carbamate(SALTDATA: FREE)
4-METHOXY-4-METHYL-2-PENTANONE
N-Ethyl-2,3-dioxo
SODIUM TETRAKIS(4-FLUOROPHENYL)BORATE
Arbutin;Hydroquinone glucose;Hydroquinone-β-D-glucopyranoside;Arbutoside
TriMethylacetaldehyde
FMoc-Thr(Bzl)-OH
O-PhosphorylethanolaMine
2-AMino-3,5-dinitro-6-Methylpyridine
Methyl 1H-pyrazole-3-carboxylate, 97%
RUTHENIUM (III) IODIDE
Propyne (ca. 3% in Heptane)
Protopanaxatriol
Lysine clonixinate
N,N-Dimethylacetamide dimethyl acetal
Iron(III) chloride hexahydrate
4-Aminophthalimide
2-MethylMercaptobenzothiazole
2-Chloro-4-Methoxypyridine-5-boronic acid
(-)-Corey lactone 4-phenylbenzoate alcohol
3,5-Diiodo-tyrosine
N-BOC-1,3-propanediamine hydrochloride, 97%
1-allyl-1H-pyrazole-4-carbaldehyde(SALTDATA: FREE)
Boc-(S)-3-amino-4-(3-benzothienyl)-butyric acid
DINOBUTON
2-(Phenylthio)ethanol
5-Chloro-1-methylimidazole
Sodium pyrophosphate decahydrate
5-Methyl-5-phenylhydantoin
1-Benzyl D-GlutaMate
Basic Green 5
5-Methyl-pyrazine-2-carboxaMide
N1-Ethyl-4-(trifluoroMethoxy)aniline, Tech.
MANNAN
beta-NicotinaMide Adenine Dinucleotide Hydrate, oxidized forM [for BiocheMical Research]
Boc-D-Ala-OH
Ethyl (R)-3-hydroxybutyrate
Aminoterphenyl
1H-Pyrrolo[2,3-c]pyridine-2-carboxylicacid, 5-methoxy-, ethyl ester
FE ELISA Kit
TLR4 ELISA Kit
APOB ELISA Kit
Human aryl hydrocarbon receptor,AhR ELISA Kit
IL-4 ELISA Kit
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit
SCF ELISA Kit
Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA Kit
Human von Willebrand Factor,vWF ELISA Kit
PFKP ELISA Kit
Dey/Engley Neutralizing Broth Base
Mycoplasma pneumonia Broth Base
HafniuM(IV) isopropoxide isopropanol adduct, 99%
CoenzyMe Q10
5-Benzyloxyindole-3-carboxaldehyde, 98%
2-Fluoro-5-hydroxybenzoic acid, 98%
Ethyl 2-mercaptoacetate
2,5-Dibromo-3-methylthiophene
2-METHOXYTETRAFLUOROPROPIONAMIDE
3-fluoro-4-methoxyacetophenone
6-Aminobenzothiazole
FMOC-L-Valine
2-BroMo-3-Fluoro-4-Picoline
2-(2,6-DiMethylphenylcarbaMoyl)benzoic acid, 97%
4-Iodotoluene, 98%
3-AMino-1-propanesulfonic acid sodiuM salt
Nitrofurazone
4-(1-Piperidinyl)benzaldehyde
PhenyltriMethylaMMoniuM broMide
5-AMINO-4-BROMO-3-METHYLPYRAZOLE HYDROBROMIDE
2-Fluoro-5-nitrobenzonitrile
6-Chloro-2,4(1H,3H)-pyrimidinedione
Ethyl 2-bromo-3-oxo-3-phenylpropanoate
3-Bromohexane (stabilized with Copper chip)
FITC I hydrochloride