LSECs细胞系;大鼠肝窦内皮细胞系大减价 货号:GD-C13118296
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-(N-[2-(tert-ButyldiMethylsilyloxy)ethyl]sulfaMoyl)benzeneboronic acid, 96%
Benzyl cinnaMate
2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiaMidite
N-OCTYL CYANIDE
3-CHLORO-2-HYDROXYPROPYL METHACRYLATE
Dibenzyl oxalate
2-Methoxy-β-nitrostyrene
2,3-Dihydro-1,4-benzodioxin-6-ylmethylamine , 95%
4-(4H-1,2,4-Triazol-4-ylcarbamoyl)benzeneboronic acid hydrochloride 98%
Lithium 3,5-diiodosalicylate, 95%
(4S,5S)-1,3-Dimethyl-4,5-diphenyl-2-[(R)-1-benzyl-2-hydroxyethylimino]imidazolidine
4-Acryloylmorpholine
2-(4-METHYLPIPERAZINO)BENZALDEHYDE
2-(3-Chlorophenoxy)-propionic acid
5-Chloro-2-nitroaniline
2-Hydrazinopyridine
(-)-1,4-Di-O-tosyl-2,3-O-isopropylidene-L-threitol
5-BroMo-8-Methoxy-2-Methylquinoline
Polycarbonate resin, approx. M.W. 45.000, pellets
Flunixin MegluMine, USP
(1alpha,3alpha,5alpha)-1,3,5-Cyclohexanetriol
N-phenyl-2-naphthylamine
9H-Purin-2-amine, 6-chloro-9-methyl-
naphthol as-bi-phosphate disodium salt heptahydrate
Linalool
2,2'-Dithiobis(benzothiazole)
Perfluorooctane
(S)-1-Boc-3-(hyroxyMethyl)piperidine
n-Propyl 3,4,5-trihydroxybenzoate, 98%
2-AMino-4-hydroxy-5,6-diMethylpyriMidine, 97%
(S)-(+)-1-Methoxy-2-propylaMine, ChiPros 98+%, ee 96+%
N-NitrosodiphenylaMine
(S)-3-AMinopyrrolidine
Zein
2-Methyl-2-propyl-1,3-propanediol
BOC-D-THR-OH
1-NAPHTHALENEMETHYLAMINE
6-bromo-3-pyridazinol(SALTDATA: FREE)
Cyclohexylamine, 99%
1,2-Diamino-2-methylpropane
2,5-Bis(4-biphenylyl)thiophene
3-CHLORO-2,6-DIBROMO-4-METHYLANILINE
DIETHYL HYDRAZODICARBOXYLATE
4-Methylthiophene-2-boronic acid
L-alpha-Cyclohexylglycine
3-Chloro-2-fluorobenzenesulfonyl chloride
Di-tert-butylphenylphosphine
2-BroMo-6-fluorobenzaldehyde
(R)-N-BOC-Prolinal, 97+%
Sulfathiazole, Powder, USP
3-BroMobenzotrifluoride
1-(2-hydroxy-3-methylphenyl)ethanone
4,4-diethoxybutylamine
Oxacillin sodium anhydrous
Benzenediazonium, 3-bromo-, tetrafluoroborate(1-)
2,5-Difluorobenzoic acid
3-BUTEN-2-OL
ETHYL (METHYLTHIO)ACETATE
(1S,2S)-trans-N-Boc-1,2-cyclohexanediaMine
3-Bromo-5-fluorobenzotrifluoride
6-Chloro-2-fluoro-3-methylbenzyl alcohol
Benzo[b]thiophene-5-carboxaldehyde
1,4-Phenylenediamine Dihydrochloride
Tetramethylammonium hydrogen sulfate
3-METHYLPHENOXYACETIC ACID
3-Chlorobenzonitrile
Nitrobenzene
3-(4-Chlorophenyl)propionic acid
(+)-Dihydrocarvone, Mixture of isoMers
Methyl 2-Chloronicotinate
2-Chloro-5-nitrobenzenesulfonyl chloride
2-CarboMethoxy-3-thiophenesulfonyl chloride, 95%
Sulfamide
2-Methyl-3-octanol
tris(4-fluorophenyl)phosphine
3,5,7-Trihydroxy-2-(4-methoxyphenyl)-8-(3-methylbut-2-en-1-yl)-4H-chromen-4-one
Tiancaian
(R)-(-)-1-cyclohexylethylamine
βAP ELISA Kit
CFD ELISA Kit
Human cath-K ELISA Kit
Human OPGL (Osteoprotegerin Ligand) ELISA Kit
3-NT ELISA Kit
NF-κB ELISA Kit
ADAMTS13/vWF-cp ELISA Kit
Human GAL-3 ELISA Kit
Human Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit
SFLT1 ELISA Kit
Eugon Agar
DPD Medium
Filter Aid, Celite AW Standard Supercel|r
XANTHOSINE 5'-MONOPHOSPHATE DISODIUM SALT
5-BroMo-2,4-Dichloro-6-MethylpyriMidine
2-Hydroxypyridine
N-phenyl-2-naphthylamine
L(+)-Glutamic acid monosodium salt monohydrate
7-Aminoindole
dimethylcarbamoyl chloride
2-Amino-6-methyl-4-pyrimidinol
Phenyl phosphine
DiphenylMethane
2,4-Difluorobenzenesulfonyl chloride, 98%
1-Boc-4-(5-iodo-2-pyridyl), 95%
3,5-Dichloro-4-fluorobenzotrifluoride
PalladiuM oxide (Ⅱ)
4-tert-ButyldiMethylsiloxy-3-penten-2-one, 95%, Mixture of isoMers
(R)-(+)-1-PhenylethylaMine
3-(DiMethylaMino)benzoic acid
N-(2,4-Dichlorophenyl)MaleaMic acid, 97%
Dimethyl trans-1,4-cyclohexanedicarboxylate, 99+%
Di-Fmoc-L-ornithine
1-Aza-15-crown 5-Ether
2,4,8,10-Tetraoxaspiro[5.5]undecane, 98+%