Hep3B2.1-7/人肝癌细胞系专注细胞培养十年 货号:GD-C12618940
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞,为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(2,4-DiMethylphenylcarbaMoyl)benzeneboronic acid, 98%
Hexadecy ltriMethyl aMMoniuM broMide
3-METHYLPENTANOYL CHLORIDE
6-Acetyl-1,2,3,4-tetrahydronaphthalene, 97%
PotassiuM 1H,1H,2H,2H-perfluoroundecanoate
ANTHRAQUINONE-2,3-DICARBOXYLIC ACID
(1R,aR)-3-Diphenylphosphino-2-(4-bis(3,5-diMethylphenyl)phosphino-2,5-diMethyl-3-thienyl)-1,7,7-triMethylbicyclo[2.2.1]hept-2-ene
Decylcyclohexane
Isothiazole-3-carbonitrile 97%
4-Propylpyridine
N-Methyliminodiacetic acid, 99%
Diallyldisulfide
Pivaloyl chloride
Sarcosine
N-Boc-trans-4-Hydroxy-L-proline methyl ester
4-Fluoro-3-methoxybenzonitrile
2',3'-Dideoxyinosine
2,5-DibroMo-4-MethyliMidazole
trans-(4-Ethoxybenzoyl)-3-acrylic acid, 98%
Phenethyl BroMide
Pentaethylene Glycol MonoMethyl Ether
3-tert-Butylphenol
6-Nitro-1,2,4-diazo acid
Ldenone Acetate
aziridine
2-BROMO-4-METHYLPHENYL ISOTHIOCYANATE
6-Chloro-5-(2,3-dichlorophenoxy)-2-methylthio-benzimidazole
2-Bromo-3-fluoroanisole
1-Heptanethiol, 98%
5^a-Cholestan-3-one, 97%
4-Methylbenzyl alcohol
SodiuM Allylsulfonate
5-(4-Methylphenyl)-1H-tetrazole
RhodiuM (triphenylphosphine)carbonylacetylacetonate
DisodiuM fuMarate
3,6-DibroMo-2,4-dichlorotoluene
Methyl (4-chlorobenzoyl)acetate
2,4-Pyridinedicarboxylic acid hydrate ,99%
5-Chloro-2-nitrophenol 95+%
Sodium carbonate monohydrate, for analysis ACS, 99.6%
2-Bromo-4-fluoropyridine
DIPROPYL MALEATE
2,2'-(BENZYLIMINO)DIETHANOL
4-Nitrobenzoic acid;Nitrodracylic acid
1-AdaMantanecarboxylic acid chloride
FMoc-Lys(Boc)-OH
1-Octen-3-one
6-Chloroquinazoline-2,4-dione
Isoxazole-5-carbonyl chloride, 90%
1,8-DIMETHYLNAPHTHALENE
Diethyl 2,5-Pyridinedicarboxylate
2-Chloro-6-methylnicotinic acid
1,10-phenanthrolin-5-amine
2-Propenyltrimethylsilane
lemongrass oil
3-Chlorobenzyl cyanide
Methyl 2-(tetrahydro-2H-pyran-4-yl)acetate
Phenylacetic acid ethyl ester
BENZOYL BROMIDE
4-(N,N-Dibenzylamino)phenylboronic acid
Ethyl 3-(trifluoromethyl)pyrazole-4-carboxylate ,97%
5,6-Dichlorobenzimidazole-1-β-D-ribofuranoside ,98%
3-(3-Bromophenyl)-5-isoxazolamine
2-Amino-5-chlorobenzoic acid
ABIETIC ACID SODIUM SALT
Chlorothiazide
3,4'-Dichlorodiphenyl ether
2,5-Dichlorophenylhydrazine hydrochloride
1,3-Dichloro-2-propanol
N-EthylsucciniMide
4-ForMyl-2-isopropylthiazole
8-Azaxanthine Monohydrate, 98%
2-METHYL-5-NITROANILINE
epsilon-Caprolactone
1-(3-Bromo-4-fluorophenyl)ethan-1-one
Triethyl 1,1,2-ethanetricarboxylate
Chrome Azurol S
Fenipentol
CLS ELISA Kit
Mouse Beta-Endorphin,β-EP ELISA Kit
Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit
Human CLDN6 ELISA Kit
OT ELISA Kit
Na-K-ATPase ELISA Kit
HSPD1 ELISA Kit
Human Estrogen Receptor,ER ELISA Kit
Human Ang1-7 ELISA Kit
CDC ELISA Kit
Trichophyton Agar #2
Modified Giolitti-Cantoni Broth Base
2-Isonitrosoacetophenone
CalciuM zirconiuM oxide, 99.2% (Metals basis)
5-AMino-2-Chlorobenzoic Acid
2-Cyclopentene-1-acetic acid, tech. 90%, reMainder Mainly 3-cyclopentene-1-acetic acid
3-tert-Butylphenol
2-Amino-1-(3,4-difluorophenyl)ethanol
N-(6-chloro-5H-purin-2-yl)acetamide
2,4-Bis(trifluoromethyl)bromobenzene
10-Deacetylbaccatin III
DOWANOL(TM) PNB
PalladiuM(II) acetate
Copper(II) carbonate dihydroxide, Cu 55% Min
2-Chloro-6-fluorocinnaMic acid, predoMinantly trans, 98%
5-BroMothiophene-2-carboxylic acid hydrazide, 98%
1,1′-Bis(diisopropylphosphino)ferrocene
Poly(4-vinylpyridine-co-ethylvinylbenzene), cross-linked
4-AMino-2-diMethylaMino-6-hydroxypyriMidine HeMihydrate, 98%
N-Benzyl 2-broMo-4-nitroaniline
CeriuM(III) nitrate hexahydrate
4-Methyl-3-nitropyridine
2-Anthracenecarboxylic Acid
Phytantriol (mixture of isomers)
Sodium orthovanadate, 99%