CNE细胞系——人鼻咽癌细胞系来源清晰 货号：GD-C11919142
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
N-Boc-DL-pipecolinic acid, 98%
SodiuM persulfate
N-CARBOBENZOXY-1,5-DIAMINOPENTANE HYDROCHLORIDE
5-BroMo-2-ethoxypyriMidine
1,3-DIIODOBENZENE
2-(ISOBUTYLTHIO)ETHANOL
2-Deoxy-D-ribose
3-Fluoro-2-methoxy-5-(methylthio)benzeneboronic acid
8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt
(S)-(-)-[(S)-2-Diphenylphosphinoferrocenyl] [2-bis(3,5-dimethyl-4-methoxyphenyl)phosphinophenyl]methanol, min. 97%
5-(2-Pyridinyl)-2-thiophenecarbonyl chloride, 97%
Zirconium carbonate oxide
Allethrin
4-Bromoaniline
α,α′-DibroMo-o-xylene
Methyl 5-Methylsulphonylindole-2-carboxylate
4,6-DiaMinopyriMidine
VariaMine Blue B DiazoniuM Salt [for Naphthol-Dyes]
Tris(diMethylaMino)borane, 98%
Silver Sulfadiazine, Micronized, USP
Benzoic Anhydride
4-Bromobenzonitrile
3-Amino-3-(4-hydroxyphenyl)propanoic acid
N-sodiohexamethyldisilazane
5-Chloro-2-fluorobenzoic acid
PRASEODYMIUM BORIDE
2-Adamantanol
Phenoxyacetic anhydride
4-n-Propoxybenzeneboronic acid, 97%
(R)-1-PhenylbutylaMine, ChiPros|r, 98%, ee 98+%
Boc-Tyr-OH
Orbencarb
2-Fluoro-5-iodotoluene
Phenyl salicylate
Isobutylene oxide
CYCLOHEXENE SULFIDE
Cyclooctylamine
2-Chloro-2-fluoropropane
2-Amino-3,5,6-trifluoroterephthalonitrile
4-(3-Nitrobenzyl), N1-BOC protected 97%
5-ETHYLIDENE-2-NORBORNENE
PALLADIUM(II) OXIDE
4,5-DIMETHYLTHIOPHENE-2-CARBOXYLIC ACID
2,3-DIMETHYL-2-CYCLOPENTEN-1-ONE
Copper(II) nitrate trihydrate
1-Phenyl-5-pyrazolone-3-carboxylic Acid
TetrabutylaMMoniuM Fluoride Hydrate [for Catalyst of acylation, silylation and cleavage of silyl ether]
2,3-Dichlorophenylacetic acid, 97%
1,3-Benzothiazole-6-carboxaldehyde, 97%
7-Dehydrocholesterol
3-Nitroaniline Hydrochloride
Beraprost Sodium
pth-4-hydroxyproline
N,N-dimethylformamide di-tert-butyl acetal
Silicon phthalocyanine dichloride
7-Azaindole
Serinol hydrochloride
2-BroMo-6-butoxypyridine
(S)-Boc-4-Methyl-β-Phe-OH
2,6-Dichloro-4-(trifluoromethyl)benzenesulphonyl chloride 95%
2-Methyl-4-nitro-1H-indole
3-(5-Methyl-tetrazol-1-yl)-phenylamine
(R)-4-Bromostyrene oxide
2,3-Dichloropropene
6-Methoxy-2-naphthol
(R)-4-PHENYL-1,3-THIAZOLIDINE-2-THIONE
N,N-DiethylforMaMide;ForMyldiethylaMine
Myricetin
1-AMinocyclopropanecarboxylic acid hydrochloride
3-(4-Hydroxy-3-Methoxyphenyl)propionic Acid
1-(3,5-Dichlorophenyl)-2,2,2-trifluoroethanone
1-(Phenylsulfonyl)pyrrole, 97%
Myristic acid
TPPS Hydrate (=Tetraphenylporphyrin Tetrasulfonic Acid Hydrate) [Ultra-high sensitive spectrophotoMetric reagent for transition Metals]
clonidine
[1,5]-NAPHTHYRIDIN-2-YLAMINE
4-Biphenylboronic acid
2-Methyl-1-butanethiol
MT ELISA Kit
VGF ELISA Kit
APOC4 ELISA Kit
Human DEFβ118 ELISA Kit
ALV-AG ELISA Kit
JUN ELISA Kit
CCKAR ELISA Kit
Human CAPZβ ELISA Kit
Human angiopoietin-like protein 3,ANGPTL3 ELISA Kit
CH50 ELISA Kit
Modified Mycoplasma Broth Medium Base（Frey）
Sensitivity Disk Broth
IndiuM(III) isopropoxide, 99+% (Metals basis), 5% w/v isopropanol
Ketotifen fumarate
5-Chloro-2(3H)-benzoxazolone, 99%
2-Fluoro-5-Nitropyridine
4-Bromobenzonitrile
Vat Black 38
Tergitol NP-33
Alpha,Alpha,Alpha-Trifluoro-m-toluic acid
4-Methylanisole
Ethopabate
3,4,5-Trifluorobenzaldehyde
N-(4-Pyridyl)thiourea, 98%
2,5-Dichlorothiophene-3-sulfonyl chloride, 97%
Tetrahydrothiophene
Deiquat Monohydrate
(ETHYLTHIO)ACETIC ACID
2-Fluorobiphenyl-3-carboxylic acid
(1,5-Cyclooctadiene)bis(triMethylsilylMethyl)palladiuM(II)
4-BroMo-3-nitrobenzonitrile
2-Methylbutyl Acetate
4-[2-(2-methyl-1H-imidazol-1-yl)ethyl]piperidine(SALTDATA: FREE)
1H-Pyrrolo[2,3-b]pyridin-5-ylamine
Orcein, certified, pure
