Wien 133细胞系——人非何杰金氏淋巴瘤细胞系如何传代

Wien 133细胞系——人非何杰金氏淋巴瘤细胞系如何传代 货号：GD-C11419283

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-BroMo-2-fluoro-M-xylene, 97%
1-Decanethiol
(-)-THUJONE TECH.
5-Chloro-2-Methoxy-3-nitropyridine
(4-CHLOROPHENYLTHIO)ACETONITRILE
(4-Hydroxyphenyl)diphenylphosphine
Ethyl 2-oxocyclohexanecarboxylate
3-Nitroaniline, 98%
3-Chloropyridine-4-carboxylic acid
1-Benzyloxy-3-methyl-2-nitrobenzene
1,3-Dimethyl-2-(2-furyl)imidazolidine
2,2'-BICINCHONINIC ACID
Amoxicillin trihydrate
1,2-DIMETHYLNAPHTHALENE
TriphenylcarbeniuM tetrafluoroborate
4-Methoxybenzonitrile
4,5-Difluorophthalonitrile
5-BroMo-2-fluoro-3-Methylbenzaldehyde
4-Nitrobenzophenone, 99+%
Tacrine Hydrochloride, Monohydrate, USP
2-Methoxybiphenyl
2-Bromo-4-fluoronitrobenzene
Pigment Yellow 55
Phenoxycycloposphazene
resveratrol
2,3,4-Trihydroxybenzaldehyde
Ethyl hydrazinoacetate hydrochloride
4-FluorocinnaMic Acid
D-Glucal, 97%
3,4-(Methylenedioxy)phenylacetonitrile, 98+%
Cobalt(II) phosphate, anhydrous
2,1,3-Benzothiadiazole
3-AMINOBENZHYDRAZIDE
N-VALERALDEHYDE 2,4-DINITROPHENYLHYDRAZONE
3,5-DIMETHYLOCTANE
N-Succinyl sulfanilaMide sodiuM salt hydrate
2,2-Di-n-octyl-1,3-propanediol
tert-Butyl 2-formyltetrahydro-1(2H)-pyridinecarboxylate
1,1-Diphenylethylene, 98%
Octyl β-D-galactopyranoside ,98%
2-MERCAPTO-4-PHENYLTHIAZOLE
6-Hydroxypicolinic acid
N-(3-AMINOPROPYL)DIETHANOLAMINE
DL-2,3-DIAMINOPROPIONIC ACID HYDROBROMIDE
2-AMinothiophenol
2-Methylquinoxaline
Nalpha-[(9H-Fluoren-9-ylMethoxy)carbonyl]-NoMega-(2,2,4,6,7-pentaMethyldihydrobenzofuran-5-sulfonyl)-D-arginine
2-AMino-5-Methylnicotinic acid
Adenosine 5'-Monophosphate sodiuM salt, 99%
8-CHLORO-CAMP
3-Methoxy-1-butanol
oxymetazoline hydrochloride
H-Lys(Boc)-OtBu . HCl
iron di(acetate)
serotonin creatinine sulfate monohydrate
2-Cyanoethyl ether
N-MethylforMaMide
3-(2-NAPHTHYLTHIO)PROPIONIC ACID
3,4-DiMethyldiphenylaMine
4-Chloro-2,6-difluorobenzaldehyde
Hypophosphorous acid, 50 wt.% solution in water
(R)-2-Isobutylsuccinic acid-1-methyl ester, 95%, (98% ee)
2-Propoxyethylamine
2-Diphenylphosphino-2'-(N,N-dimethylamino)biphenyl
4-Bromo-2-hydroxybenzoic acid
1-Amino-8-hydroxynaphthalene-3,6-disulphonic acid
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
2,6-Difluorobenzenesulfonyl chloride
1-Butyl-3-MethyliMidazoliuM dibutyl phosphate
Idebenone
1-Isobutyl-3-(trifluoroMethyl)pyrazole
2,3,4-Trifluorobenzaldehyde, 98%
Disodium succinate hexahydrate
2,5-DiMethylbenzothiazole
1,4-dibromobut-2-ene
alpha-Bromo-o-tolunitrile
chloroethylmethylsilane
Methyl 3-oxotetrahydrothiophene-2-carboxylate
LEP ELISA Kit
Mouse substance P receptor,SP-R ELISA Kit
Human protocadherin 1,PCDH1 ELISA Kit
Human DPP8 ELISA Kit
Chicken 5-Nucleotidase,5-NT ELISA Kit
β-DF ELISA Kit
Irna ELISA Kit
Human PROC ELISA Kit
Human Serotonin,ST ELISA Kit
Hp-IgG ELISA Kit
Aminoacid Decarboxylase Test Medium controls
Kligler Iron Agar
PalladiuM(II) 2,4-pentanedionate, Pd 34.7%
HolMiuM(III) fluoride, anhydrous, 99.9% (REO)
5-(TrifluoroMethoxy)salicylaldehyde, 98+%
FurfurylaMine
2-Bromo-4-fluoronitrobenzene
Pyridine, 3,5-dibromo-4-chloro-
Vitamin A
dipropoxymethane

M-CRESYL ACETATE
Rapamycin
4-Methyl-1,3-Oxazole-5-Carboxylic Acid
PotassiuM antiMonate trihydrate, 94+%
3-Nitro-4-(1-piperidinyl)benzaldehyde, 96%
4-Hydroxybenzoic acid, 99%
4-AcetaMido-2-aMinobenzenesulfonic acid hydrate
1,5-HEXADIENE DIEPOXIDE
Methyl 5-cyclopropyl-1-(pyridin-2-yl)-1H-pyrazole-4-carboxylate
Ethyl 3-hexenoate
AMberlite IRC-50(H), ion exchange resin
N-(4-NITROPHENYLSULFONYL)-L-PHENYLALANINE
9-Bromophenanthrene 98%
1,2-Epoxydodecane
4-(4-Bromo-1H-pyrazol-1-yl)benzenesulphonamide 97%