NCI-H716[H716]细胞系——人结直肠腺癌细胞系如何传代

NCI-H716[H716]细胞系——人结直肠腺癌细胞系如何传代 货号：GD-C11419093

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Isobutyl Methacrylate, 99.5+%, stab. with 10ppM 4-Methoxyphenol
3-Nitrophenyl disulfide
Poly(ethylene glycol) Methyl ether acrylate
CHLOROMETHYL PHENYL SULFONE
2-ETHYLTHIO-1,3,4-THIADIAZOLE-5-THIOL
2-METHYL-4-UNDECANONE
2,4-BIS(TRIFLUOROMETHYL)CHLOROBENZENE
Zinc nitrate hexahydrate, min. 98%
4-Methylphthalonitrile
N-n-Propyl-N-(2,3-epoxypropyl)perfluorooctyl-sulfonamide
3-(4-Bromophenyl)-1,2,4-oxadiazole 96%
2-CHLORO-4,6-DIAMINO-1,3,5-TRIAZINE
2-Chloro-4,6-dimethylpyridine
N-Hydroxymethyl-3,4,5,6-tetrahydrophthalimide
N1-(2-Chlorophenyl)-2-chloroacetaMide
2-MethylcyclohexylaMine (Mixture of isoMers)
DiMethyl (1-Diazo-2-oxopropyl)phosphonate
4-Methoxybenzyl 5-broMo-2-chlorobenzaMide
Tri-n-butyltin hydride, 97%
Pyronin Y
PhthaliMidoacetone
3-Acetamidophenol
4-methoxyphenylacetaldehyde
4'-tert-Butylacetophenone
2-Bromo-1-chloro-3-(trifluoromethyl)benzene
Dodecanamine
Mancozeb
2,5-Dichloroterephthalic Acid
4,4-Difluorocyclohexanecarboxylic acid, 98%
3,3'-DiMethoxybenzil, 99+%
3,5-DiMethylpyridine-4-boronic acid pinacol ester, 95%
3-(broMoMethyl)-5-chlorobenzo[b]thiophene
2-ISOBUTYLPYRAZINE
-1,4-bis(2-ethanesulfonic Acid) DisodiuM Salt [Good's buffer coMponent for biological research], 98%
1-(4-PyridinoMethyl)pyrazole-4-boronic acid pinacol ester
N-ETHYL-N-(2-HYDROXYETHYL)NITROSAMINE
4-[(3-Bromophenyl)sulphonyl]morpholine 95%
2-(tert-Butylamino)-3,6,7-trichloroquinoxaline 96%
Tetraphenylphosphonium bromide, 99%
3-Iodobenzonitrile
2-Tetrahydrofuran-2-ylethanamine
2-Amino-5-chlorophenol
4-METHOXY-1-INDANONE
1-Hexanethiol
Methyl 5-acetylsalicylate
D-(+)-Galactose anhydrous
2,9-Dichloro-1,10-phenanthroline
2-chloro-5-fluoropyridine-4-carbaldehyde
Crotyl chloride, 70%, tech.
TrisMat(R) Hydrochloride, Crystal, Reagent
3-tert-Butylphenol
1,1,1,2-tetrafluoroethane
1H-Pyrazolo[3,4-b]pyridin-3-amine
Sulphur Brown 10 -Solubilised
trans,trans-1,4-Diphenyl-1,3-butadiene
GALLIUM(III) OXIDE
2,4-Ditert-butoxypyriMidine-5-boronic acid
N-Boc-1,2-phenyldiaMine
N-ACETYL-2-(4-NITROPHENYL)ETHYLAMINE
2,6-Difluoro-3-hydroxybenzoic acid
3-Chlorobenzoyl isothiocyanate
5-Methoxy-2,1,3-benzoxadiazole, 97%
5-Bromo-2-chloro-4-pyridinamine
1-Ethylimidazole
DL-Homocysteinethiolactone hydrochloride
NEROL
2-Decanone;2-Oxodecane
2',7'-Dichlorofluorescein
16,17-alpha Epoxypregnenolone
5-Norbornene-2-MethylaMine (Mixture of isoMers)
5-Methyl-3-p-tolyl-1,2,4-oxadiazole
Bis(benzene)chroMiuM, 97%
DOPAMINE HYDROCHLORIDE
Flupirtine Maleate Salt
Corilagin
2-[3-(2-Amino-ethyl)-adamantan-1-yl]-ethylamine
Geraniol
Sulfuric acid
PP ELISA Kit
α2-AP ELISA Kit
Human EEA1 ELISA Kit
Human mast cell tryptase,MCT ELISA Kit
Chicken LH (Luteinizing Hormone) ELISA Kit
Rat TGF-beta-inducible early response gene-1,TIEG1 ELISA Kit
Chicken virus enteritis,DEV ELISA Kit
Human OXTR ELISA Kit
Human VEGFR-2/Flk-1 ELISA Kit
ACE2 ELISA Kit
Murashige & Skoog Medium without Agar (1/2 Sucrose
Glucose Blood Agar
Iron(III) isopropoxide, 98%
Glycine hydrochloride
5-Chloro-2-hydroxybenzyl alcohol, 98%
2-Fluoro-N-(2,4-difluorophenyl)benzaMide, 97%
3-Acetamidophenol
C.I. Reactive brown 11
tween(R) 65
3-Fluoro-4-nitrophenol

3-BROMO-4-NITROPYRIDINE N-OXIDE
2-Picoline
1,2,3-Tri-O-Acetyl-5-Deoxy-Beta-D-Ribofuranose
2,4-Pentanedione dioxiMe, 98+%
Methyl Methanesulfonate, 99%
p-TolylMagnesiuM broMide
Candelilla wax
2,6-DIHYDROXYANTHRAQUINONE
1-BOC-7-Methylindole
4-Butyrylphenylboronic acid
3-(Phenylsulfonyl)propanoic acid
3-Bromophenylacetonitrile, 98+%
3-Undecylthiophene
4-Ethylbenzenesulphonamide 98%
Coelenterazine hcp