Hep 3B2.1-7[Hep 3B,Hep-3B,Hep3B]细胞|人肝癌细胞系什么细胞 货号:GD-C10520420
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4'-BroMoheptanophenone, 97%
Salicylanilide
ETHYL HYDROGEN GLUTARATE
6-Chloro-2-fluoro-3-Methylbenzonitrile, 97%
4-Methoxy-2,3,6-triMethylbenzenesulfonyl chloride, tech. 90%
2',4'-DICHLOROPROPIOPHENONE
1-MethyliMidazole
Dicarbonylacetylacetonato iridium(I), 98%
2-(tert-Butoxycarbonylthio)-4,6-dimethylpyrimidine [Boc Agent for Peptides Synthesis]
1-Phenyl-propylamine
3-Fluorobenzyl mercaptan
2-Naphthoxyacetic acid
5-CHLORO-M-PHENYLENEDIAMINE
METHYL 2-CHLOROISONICOTINATE
4-[4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)phenyl]Morpholine
3,4-DichlorophenethylaMine
4-BroMo-2,3-difluorophenol
Methyl 3-broMoiMidazo[1,2-a]pyridine-6-carboxylate
ZirconiuM(IV) oxide, 98.5%
Sudan II
Undecanoic Acid
o-Tolylacetic acid
D-Histidine hydrochloride monohydrate
5-(3-dimethylaminopropyl)-10,11-dihydrodibenzo(a,d)cyclohepten-5-ol
1,2,5,6-tetrahydropyridine-3-carboxylic acid
2-ETHYL-4-METHYLIMIDAZOLE
SARCOPHYTOL A
N-Benzoylcytidine
3,5-Diphenyl-2-thioxo-2,3-dihydrothieno[2,3-d]pyriMidin-4(1H)-one, 96%
3-(TrifluoroMethoxy)benzyl alcohol, 98+%
2,3,4-Trifluorophenol
5-Chloro-2-fluoroaniline
9-Phenanthreneboronic Acid (contains varying aMounts of Anhydride)
Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
DiallylaMine
4-tert-Butyl-2,6-diisopropylphenol
3-Chloro-2,6-difluorobenzoyl chloride
2,6-Diisopropylphenol ,99%
2,6-Dichlorotoluene, 99+%
3-Methoxypyridine-5-boronic acid pinacol ester
AMINOGUANIDINE HYDROCHLORIDE
ZINCON
2,3,4,5,6-PENTAFLUOROPHENOXYACETYL CHLORIDE
Neodymium trifluoride
3-Nitrotoluene
1-Butyl-3-MethyliMidazoliuM thiocyanate
L-Leucyl-L-tyrosine
N-(5-BroMopyridin-2-yl)pyrrolidine
(4-Methyl-2-phenyl-1,3-thiazol-5-yl)Methanol, 97%
ISOPROPYL BUTYRATE
Tetrakis(1,1,1,3,3,3-hexafluoroisopropyl) Orthosilicate
piperidine-4-propanol
[1S-(1α,4aα,6α,7α,7aα)]-1-(β-D-glucopyranosyloxy)-1,4a,5,6,7,7a-hexahydro-6-hydroxy-7-methylcyclopenta[c]pyran-4-carboxylic acid
4-(prop-2-en-1-yloxy)phenol
Benzyl glycidyl ether
BOC-D-3,4-Dichlorophe
RubidiuM fluoride
4,4'-ISOPROPYLIDENEDIPHENOXYACETIC ACID
1-(4-Chlorophenyl)-1-cyclobutanecarbonitrile
2-Azidotoluene solution
1-Naphthalenemethylamine, 97%
3-Methoxyphenol, 97%
(Chloromethyl) phenyl ethyl dimethylchlorosilane
Chromone-3-carboxylic acid, 95%
2-Ethylfuran
3-Ethylpyridine
N,N-DI-N-BUTYLETHYLENEDIAMINE
Ethyl N-benzyl-3-oxo-4-piperidine-carboxylate hydrochloride
1-BroMoadaMantane
2-(4-Nitrophenyl)-4,4,5,5-tetraMethyliMidazoline-3-oxide-1-oxyl Free Radical
3-(4-BroMophenyl)-1,2,4-oxadiazol-5(4H)-one
SodiuM ethoxide, 96%, pure
Loratadine, USP
2-Hydroxy-3-Methyl-2-cyclopentenone
3-Fluoro-4-nitrobenzoic acid
Methyl 4-amino-3-nitrobenzoate
3-chloropentane
Formanilide
STK ELISA Kit
S100B ELISA Kit
Human PCDHα8 ELISA Kit
Human DNM2 ELISA Kit
Chicken L-Phenylalanine ammonla-lyase,PAL ELISA Kit
Rat GLβ (Galactosidase, Beta) ELISA Kit
sE-selectin ELISA Kit
Human Elastin,ELN ELISA Kit
HSA ELISA Kit
APT ELISA Kit
Sodium Sulfite Agar
T1N3 Broth
Dimethyl acetylsuccinate
Nickel(II) fluoride, anhydrous, 97%
5,5-DiMethylhydantoin, 97%
2-Fluoro-3-nitropyridine
o-Tolylacetic acid
Ammonium perrhenate(VII)
Oryzanol
2,6,6-trimethylcyclohepta-2,4-dien-1-one
Trimethylboroxine
N-(4-HYDROXYPHENYL)METHACRYLAMIDE
3-Chlorocinnamic acid
N^a-Boc-N^d-FMoc-L-ornithine, 95%
4-(3-Pyrrolidinyl)Morpholine, 97%
D-(+)-Xylose
Diphenylphosphine oxide
2-Mercapto-5-Methoxythiazolo[4,5-b]pyridine
1,4-DIIODO-2,3,5,6-TETRAMETHYLBENZENE
(S)-(+)-2-Hexanol
DODECYL ACETATE
2-Benzyl-4-chlorophenol , 97%
Fumaramide, 98%
Ethyl 2-amino-1,3-oxazole-5-carboxylate 95%
2,3,6-Trifluorophenylacetonitrile