SW579[SW579,SW-579]细胞|人甲状腺鳞癌细胞系什么细胞 货号:GD-C10519034
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞,为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Mercaptothiazoline, 98+%
3-(Methylthio)hexyl acetate
ISOBUTYL TIGLATE
6-BroMo-3-Methylquinazolin-4(3H)-one
2-Fluoro-3-(trifluoroMethyl)phenol
5-(2,5-Dichlorophenyl)-1H-tetrazole, 97%
Ethyl 4-aMinocinnaMate
Ethyl Eosin, potassium salt, pure
4-(1H-Pyrazol-1-yl)phenyl isothiocyanate 97%
Zn(II) meso-Tetra (N-methyl-4-pyridyl) Porphine Tetrachloride
2,2,3,3,3-Pentafluoropropyl 2-fluoroacrylate
2-Chlorophenyl cyclopentyl ketone
3-AMINO-6-METHOXY-2-METHYLAMINO-PYRIDINE, DIHYDROCHLORIDE SPECIALITY CHEMICALS
2-Methoxypropene
(R)-(-)-2-Phenylpropionic acid
3-Chlorobenzyl BroMide
N,4'-DiMethylforManilide
Ethyl 7-broMo-1h-indole-2-carboxylate
Triethyl phosphate, 99%
PotassiuM Nitrite, Crystal, Reagent, ACS
(S)-(-)-1-Methyl-2-(1-naphthylaMinoMethyl)pyrrolidine
2,4,6-Triiodophenol
Calcium disodium EDTA
cis-1,2,3,6-Tetrahydrophthalic anhydride
2-Fluoro-4-methoxybenzaldehyde
2,4-Dinitro-1-(trifluoromethoxy)benzene
Promethazine hydrochloride
5-ForMyl-2-Thiopheneboronic Acid
6-Nitropiperonyl alcohol, 98+%
5-Chloro-6-hydroxynicotinic acid, 98%
Ethyl 4-Iodobenzoate
3-Iodoanisole
3-AMINO-4,5-DIHYDRO-1-PHENYLPYRAZOLE
POTASSIUM METHANESULFONATE
3-(DIFLUOROMETHOXY)BROMOBENZENE
Boc-Gly-OtBu
2,5-Difluoroanisole
2-[2-(Bromomethyl)phenyl]thiophene, 95%
4-Methyl-2-phenyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,3-thiazole, 97%
2-Chloro-3-fluoroisonicotinic acid
6-Fluoro-2-tetralone
Bromocriptine mesylate
Osthole
TANTALUM(V) OXIDE
2,6-Difluorobenzoic Acid
Dibenzothiophene sulfone
Methyl 4,6-O-Benzylidene-2-deoxy-alpha-D-erythro-hexopyranosid-3-ulose OxiMe
2-(ChloroMethyl)-1,3-benzothiazole
6-(4-Chloro-3,5-diMethylphenoxy)pyridin-3-aMine, 90%
ETHYLENE GLYCOL MONOSTEARATE
L-Phenylalanine
chloromethyl-[chloromethyl-bis(trimethylsilyloxy)silyl]oxy-b...
Malicaciddimethylester; 98%
3,5-Dimethoxyphenylacetonitrile
O,O-diethyl thiophosphate, potassium salt
TETRA-N-PROPYLTIN
4-AMinobenzo-2,1,3-thiadiazole
1-(2,4-DIMETHYLPHENYL)-2-THIOUREA
2-(N,N-BisBOC-AMino)pyriMidine-5-boronic acid, pinacol ester
3-(2-Thienyl)aniline, 95%
5,6-Dimethoxyindole 99%
3-methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine(SALTDATA: FREE)
Dichloro[(S)-(-)-2,2'-bis(diphenylphosphino)-1,1'-binaphthyl][(1R,2R)-(+)-1,2-diphenylethylenediamine]ruthenium(II), min. 98%
2-Amino-5-formylthiazole
4-Acetylbenzenesulphonamide
N,N-Diethyl-1,1,2,3,3,3-hexafluoropropylamine
cis-2-Pentene;1-Ethyl-2-Methylethylene;
PlatinuM(IV) oxide Monohydrate
1,5-Bis(acryloyloxy)pentane (stabilized with MEHQ)
2-Fluoro-5-pentanoyl-1,3-phenylenediboronic acid, pinacol ester
DiMethyl chlorophosphate, 96%
3-(4-Methoxyphenyl)-5-Methyl-4-isoxazolecarbonyl chloride, 97%
2-Methyl-3-(3,4-Methylenedioxyphenyl)propionaldehyde
1,2-Epoxycyclooctane
1,2-cyclopentane dicarboxylic acid
Cyclopropylboronic acid methyliminodiacetic acid anhydride
N,N-Dimethylazetidin-3-amine
N-(3-bromobenzyl)propan-1-amine
CCR ELISA Kit
α-glucosidase ELISA Kit
Human MUC6 ELISA Kit
CPn IgG ELISA Kit
Chicken Nuclear factor-kappa B,NF-κB ELISA Kit
Rat Soluble protein-100B,S-100B ELISA Kit
IL1β ELISA Kit
Human CRF ELISA Kit
VEGF ELISA Kit
ANP ELISA Kit
Glfu Anaerobic Medium Semisolid without Dextrose
Czapek Yeast Exatract Agar
TitaniuM(IV) isobutoxide
Heparin sodium
5-Chloropyridine-2-boronic acid, 96%
2-Fluorobenzoic Acid Ethyl Ester
2,4,6-Triiodophenol
Capreomycin
Antimony tribromide
adrenosterone
CHLOROAURIC ACID HYDRATE
2,6-DIMETHYL-3-HYDROXYPYRIDINE
2-Hydroxy-3-AMino-5-Picoline
IsobutyltriMethoxysilane, 97%
4-Acetyl-5-Methyl-1-phenyl-1H-pyrazole, 97%
1-Benzothiophen-2-ylboronic acid
N-EthylMaleiMide
4-Pentyloxy-[1,1'-biphenyl]-4'-carbonitrile
5-FLUORO-2-(TRIFLUOROMETHYL)BENZONITRILE
DiMethyl 2,6-dibroMoheptanedioate
2-FURFURYL ISOTHIOCYANATE
2-Methylbenzeneboronic acid
4-Fluoropentylbenzene
tri-n-Butylmethoxytin
4-Butyrylbiphenyl