RWPE-2细胞|人前列腺正常细胞系什么细胞 货号:GD-C10519176
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Hydrazinobenzenesulfonic acid hydrate, 98%
Vernolat
3-Cyclohexene-1-carboxaldehyde
Boron triiodide
1-(FMOC-AMINO)CYCLOHEXANECARBOXYLIC ACID
2-Chloro-6-Methylbiphenyl-3-carboxylic acid
4-AMino-2-picoline
4-Hydroxypyridine-2,6-dicarboxylic acid hydrate ,97%
6-Undecanone
5-Iodo-3-nitropyridin-2-ol
1-(4-Hydroxy-phenyl)-cyclopentanecarboxylic acid
DL-2,4-DIAMINOBUTYRIC ACID DIHYDROCHLORIDE
5-Hydroxypyrazine-2-carboxylic acid
4-Hydroxybenzhydrazide
2-(TriMethylsilyl)thiazole
4-(2-Morpholin-4-ylethoxy)aniline
1,4-Di(2-thienyl)-1,4-butanedione
Xylene Fast Yellow 2G
TyraMine hydrochloride, 99%
SodiuM Ferrocyanide, Reagent
2,6-Bis[(2S,5S)-4,4-diphenyl-1-aza-3-oxabicyclo[3.3.0]octan-2-yl]pyridine
Dithiocarbonicaciddiethylester
N-Benzyloxycarbonyl-D-serine
2,6-Lutidine-N-oxide
3-Fluoro-4-methylphenol
PERFLUORO-N-HEPTYL IODIDE
5-Nitroisoquinoline
2,5-DibroMo-3-Nitropyridine
L-Aspartic acid 1-Methyl ester, 98%
^a-D-Glucose pentaacetate, 99%
2,3-Difluoro-6-nitrophenol
AcetobroMo-α-D-glucuronic acid Methyl ester
2-PIPERIDINOACETAMIDOXIME
Perfluorobutyl iodide
Chloroplatinic acid
SodiuM anthraquinone-2-sulfonate
4-Chloro-4'-fluorochalcone, 98%
1,3-Dimethyl-1H-pyrazole-4-carbaldehyde
5-Chloro-3-nitrosalicylaldehyde
2-Chloroethylurea
Tetramethylammonium fluoride
1,2-Dibromo-3-butanone
4-(4-Fluorobenzoyl)butyric acid
SodiuMMetaphosphate
1,4-Dihydroxyanthraquinone
Methyl 5-chloro-2-pyrazinecarboxylate
Silybin (Mixture of Silybin A and Silybin B)
1-BroMo-3-iodobenzene, 98+%
1,2,3-Trichloro-4-nitrobenzene, 97%
ETHYL EOSIN
Pyrazinecarboxylic Acid
(2,6,6-trimethylbicyclo[3.1.1]heptan-3-yl)borane
Carvedilol
methyl 5-methylpyridine-2-carboxylate
4-Pyrimidinecarbonitrile, 2-chloro-6-methyl-
1,1-Cyclobutanedicarboxylic acid
2,4-BIS(ANILINO)-6-(4-MORPHOLINO)-1,3,5-TRIAZINE, 97%
O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE
1,2-Difluorobenzene
2-(4-Formylphenoxy)acetamide
4-Vinylbenzoic Acid (stabilized with BHT)
4-Methyl-4-heptanol
Galangin
1-METHOXY-3-TRIMETHYLSILOXY-1,3-BUTADIENE
3-Fluoro-5-bromotoluene
3-Bromo-2-(bromomethyl)propionic acid
2-Benzyl-2-(dimethylamino)-4'-morpholinobutyrophenone
(2,3,4,5,6-Pentafluorophenyl)Methanol
1-Benzyl-3-(diMethylaMino)pyrrolidine
Ethyl 5-hydroxy-2-phenylbenzofuran-3-carboxylate
AMberlite? IRA-402, Cl-forM, ion-exchange resin
Methyl 5-broMo-2-(Methylsulfanyl)-4-pyriMidinecarboxylate, 97%
TetraMethyl 1,1,2,3-Propanetetracarboxylate
N-SucciniMidyl BroMoacetate
2-Hydroxyphenylacetic acid
N-Boc-4-iodopiperidine
PERLITE
4,5-dimethyl-2-isobutyl-3-thiazoline
PYGL ELISA Kit
TIEG1 ELISA Kit
Human APOA2 ELISA Kit
Human ARSH ELISA Kit
Chicken IL-23 (Interleukin 23) ELISA Kit
αHSG ELISA Kit
BMP4 ELISA Kit
Human monocyte chemotactic protein 3,MCP-3 ELISA Kit
Human ANG ELISA Kit
PF4 ELISA Kit
Brain Heart Infusion Agar
Triple Sugar Iron Agar Medium
Isocytosine, 99%
Flutamide
5-Benzyloxy-1-Boc-indole-2-boronic acid, 98%
2-Fluoro-5-Iodobenzoic Acid
Dithiocarbonicaciddiethylester
5-amino-8-quinolinol
2,2,4-trimethyl-1-oxa-4-aza-2-silacyclohexane
3-chlorobenzenediazonium tetrafluoroborate
1-Methyl-2-pyrrolidinone
N-Methyl HoMo
2-Ethyl-2-oxazoline, 99%
2-Chloro-5-nitrobenzoic acid, 98+%
Methyl 4-chlorocarbonylbenzoate, 98+%
IMinodiacetic acid
3,5-DINITRO-4-METHYLBENZENEBORONIC ACID
DIBENZYL SUCCINATE
DIMEDONE DIOXIME
3-FLUORO-4-(TRIFLUOROMETHYL)BENZALDEHYDE
4-Isobutyl-3-nitrophenylboronic acid
2,3,6-Trifluorobenzoic acid 98%
4-[(2-Methoxy-2-oxoethyl)aminocarbonyl]benzeneboronic acid 97%
4-Bromo-4',4''-dimethyltriphenylamine
2-Cyano-5-methoxybenzoic acid methyl ester