CCD-1095Sk细胞|人乳腺浸润性导管癌旁皮肤细胞系什么细胞 货号:GD-C10519149
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
cis-1,2-Bis(diphenylphosphino)ethylene, 97%
SodiuM 1-hexanesulfonate
1,3-BIS(4-HYDROXYBUTYL)TETRAMETHYLDISILOXANE
N-(Tetrahydro-2-oxo-3-thienyl)-acetaMide
BariuM hydroxide hydrate
1-BroMo-3-(MethoxyMethyl)benzene
4,4'-DiaMinodiphenylsulfone
Benzoyl isothiocyanate
(Isocyanoimino)triphenylphosphorane
1H,1H-Perfluoroundecan-1-ol 95+%
2-Methyl-1-phenylpropene
2-Butyl-1H-imidazole-4-carbaldehyde
2,4-DIMETHYLPHENYL ISOTHIOCYANATE
Testosterone isocaproate
2-BroMoethylaMine HydrobroMide
Allyl chloride
DerMatan Sulfate SodiuM Salt
2-Vinyl-4,6-diaMino-1,3,5-triazine
Tris(triMethylsilyl) borate
SodiuM Acetate, Trihydrate, Technical
1,3,5-TriMethylcyclohexane
Furfuryl acetate
Bis(4-aminophenoxy)ethane
Zirconium n-butoxide
2-Fluoro-4-methoxybenzoic acid
1-Aminopiperidine
Ethyl (R)-(+)-4-chloro-3-hydroxybutyrate
Ellagic acid
Benzoylferrocene, 98%
3',4'-Dihydroxyflavone, 97%
D-(?)-Erythrose
O-tert-Butyl-D-tyrosine
LUTETIUM(III) IODIDE
Phenyl 3-hydroxy-2-naphthoate
TRANS-2,4-DICHLOROCINNAMIC ACID
DipotassiuM Glycyrrhizinate Hydrate, 75%
2,4-Difluorothiophenol 97%
2-Naphthaldehyde
1-Octadecene
3-(2-Chloro-phenyl)-3-oxo-propionic acid ethylester
Copper(II) borofluoride
2,6-DICHLORO-4-METHYLNICOTINAMIDE
Trimethoxyboroxine
2-[N-(2-Cyanoethyl)anilino]ethyl acetate
Octyl 4-MethoxycinnaMate
Diethyl diMethylMalonate
1-Ethoxy-4-hexyloxybenzene
4-Chloro-3-fluorophenylacetic acid
5-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-indole, 97+%
DI(ETHYLENE GLYCOL)
N-MethylMaleiMide
Ethylenediamine Tetramethylenephosphonic acid
Halquinol
5-Fluoro-2(1H)-pyrimidinone
alpha,alpha-dibromo-4-nitrotoluene
2-Chloro-2-methylpropane
1-AMINO-4-(2-NITROBENZENESULFONAMIDO)BUTANE
DL-ALANYL-L-ALANINE
3-(TrifluoroMethoxy)broMobenzene
Methyl 2-formylbenzoate , 97%
2,3-Dihydro-1,4-benzodioxine-2-carboxylic acid, 97%
4-N-Boc-amino-3-fluorobenzaldehyde
(6-methyl-2-oxo-2,3-dihydro-1H-indol-3-yl)acetic acid(SALTDATA: FREE)
4-Bromophenetole
SEC-BUTYLAMINE
2-Mesitylenesulfonyl chloride
BROMOBENZENE-D5
5-Fluoro-2-nitrophenol
1-AMinopiperidine
Isobutyraldehyde 2,4-Dinitrophenylhydrazone (1Mg/ML as Aldehyde in Ethyl Acetate) [for Offensive Odors Analysis] (1ML*5)
N,N-DiMethyl 4,5-diiodo-1H-iMidazole-1-sulfonaMide
7-Nitroindole, 99%
OKADAIC ACID AMMONIUM SALT
5-BroMo-2-pyridinecarboxylic Acid
2,4,6-Trimethylbenzeneboronic acid
DL-Serine, O-methyl-
Methyl oxalyl chloride
dihydro-5-octylfuran-2(3H)-one
HABP1 ELISA Kit
UGCG ELISA Kit
Human Apo-C2 ELISA Kit
Human DEFβ103B ELISA Kit
Chicken Interleukin 9,IL-9 ELISA Kit
COL11α1 ELISA Kit
DA ELISA Kit
MAOA ELISA Kit
Human Arresten ELISA Kit
FPA ELISA Kit
Lecithin Tween 80 Nutrient Agar Medium
Medium 8
ANTIMONY (III) ISOPROPOXIDE
DiMethyl fuMarate, 99%
5-Chloro-1-MethyliMidazole
2-Fluoro-5-nitrobenzoic acid, 98%
Furfuryl acetate
(trimethylsilyl)methylmagnesium chloride
Allyl methyl sulfide
alpha,alpha,alpha-Trifluoro-p-tolualdehyde
2-Picoline
Polystyrene
2-Hydroxy-3,5-dinitropyridine, 98+%
N-Boc-L-valinol, 97%
ALUMINUM LANTHANUM ISOPROPOXIDE
3-BroMobenzyl broMide
FMoc-Cys(Bzl)-OPfp
FMoc-3-(trifluoroMethyl)-D-phenylalanine
5-Benzyloxy-2-chlorophenylboronic acid
2,3-Dihydrobenzofuran-5-carboxaldehyde
5-(3-Sulphamoylphenyl)furan-2-carboxaldehyde
4-bromo-1H-pyrazole-5-carboxylic acid(SALTDATA: FREE)
Dimethyldineodecanoatetin