SV-HUC-1细胞|人输尿管上皮永生化细胞系(买细胞,送话费,多买多送) 货号:GD-C10419215
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
tert-Butyl 2,2,2-trichloroacetiMidate, 97%
N-Phenyl-4-biphenylaMine
5-Chloro-2-hydroxybenzoic acid
2,4,6-TriMethoxyphenylacetic acid
Decafluorobenzophenone, 98%
N-Boc-pyrrole-2-boronic acid MIDA ester
1,1-DiMethylpropylMagnesiuM chloride solution
2-(1H-Pyrazol-1-yl)benzylamine 97%
Methyl [6.6]-Phenyl-C61-butyrate
Iridium(III) chloride, anhydrous
4-amino-5-pyrimidinecarboxamide(SALTDATA: FREE)
5,7-DICHLORO-4-HYDROXY-2-(TRIFLUOROMETHYL)QUINOLINE
4-Nitropyridine N-oxide
N-(6-BROMOHEXYL)PHTHALIMIDE
Cyclohexylboronic acid
Hydridotetrakis(triphenylphosphine)rhodiuM(I)
2,5-Difluoroanisole
Benzyl 3-forMylpiperidine-1-carboxylate
VinylMagnesiuM chloride, 1.9M (16.5 wt.%) solution in THF, AcroSeal
SodiuM Tetraphenylborate, Reagent, ACS
trans-2,4-DichlorocinnaMic Acid
Trithiocyanuric acid
3-(1H-indol-2-yl)aniline
Benzene-d6
2-Pyridinecarboxylic acid, 6-fluoro-, methyl ester
3-Methyl-4-nitroaniline
1-BroMohexadecane
Diethyl acetaMidoMalonate, 98+%
2-Chloro-6-fluoro-3-Methylbenzyl broMide, 97%
BroMoethane, 98%
1-(2-AMinoethyl)pyrrolidine
3-METHOXY-4-METHYLANILINE
COPPER(II) HYDROXYFLUORIDE 99.999%
PaMoic acid
Tiglic acid
Ethyl oxazole-4-carboxylate
3-Chloro-5-fluorobenzylbromide
Sodium 4-(chloromercuri)benzenesulphonate
Tris(2,2,6,6-tetramethyl-3,5-heptanedionato)dysprosium(III), 98+% (99.9%-Dy) (REO) [Dy(TMHD)3]
Ethyl 2-cyclohexylidenacetate
GLYOXIME
Tricobalt tetraoxide
12-HYDROXYSTEARIC ACID
NAPHTHOL AS-D CHLOROACETATE
Quinoline
3-BroMo-2-naphthol
3-(2,2-Dicyanovinyl)phenylboronic acid
Copper(II) sulfate, 98%, pure, anhydrous
(1S,2S)-2-(Diphenylphosphino)-1,2-diphenylethylaMine, 97%
2,6-DiMethoxybenzoic Acid
3-(N-Acetyl-N-MethylaMino)pyrrolidine
tri-n-butyltin methanolate
4-bromo-1,2-dimethyl-1H-imidazole
Propionyl-L-Carnitine HCl
trans-(5RS,6SR)-6-amino-2,2-dimethyl-1,3-dioxepan-5-ol
Niobium oxide
N-p-Tosylglycine
4-MethoxybenzaMide
SulfabenzaMide
2-Hydroxyethyl methacrylate, stabilized, 97%
Sodium nitrite, 97+% (ACS)
5-Chloropyrazine-2-carbonitrile
Methyl 5-(Hydroxymethyl)-1H-imidazole-4-carboxylate
Tetrasodium Ethylenediaminetetraacetate Dihydrate
Ethyl formate
N,N-DIPHENYLFORMAMIDE
3-(TRIFLUOROMETHYL)BENZOPHENONE
2-Acetoacetoxyethyl Methacrylate
1-BroMo-2-ethylbutane
4,4-DiMethylcyclohexanone
N-Phenyl 5-broMo-2-chlorobenzaMide
2,3-Dihydro-1,4-benzodioxine-6-carbaldehyde, 97%
Tretinoin, Powder, USP
HistaMine Diphosphate Hydrate
2-Acetyl-1-naphthol
4-(4-Fluorophenyl)piperidine hydrochloride
Dodecylbenzenesulfonic Acid
trielaidin (C18;1),(trans)-9
PP ELISA Kit
Slit2 ELISA Kit
Human Pg (Progesterone) ELISA Kit
Human DUB ELISA Kit
Chicken Interleukin 1,IL-1 ELISA Kit
α-SMA ELISA Kit
Chicken TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
Human choline phosphoglyceride,PC/CPG ELISA Kit
Human heme oxygenase 1,HO-1 ELISA Kit
Guinea pig IGF-2 (Insulin Like Growth Factor 2) ELISA Kit
Fluid soybean-casein Digest Broth
TGC Medium without Indicator, Liquid
Ethyl acetoacetate, 99+%
IRON(III) FLUORIDE TRIHYDRATE
5,6-DiMethoxyindole, 98%
2-Fluoro-4-hydroxypyridine, 97%
Trithiocyanuric acid
4-(2-Aminoethyl)pyridine
Calcium sulfite
2,6-Pyridinedicarboxylic acid
2-CHLORO-6-FLUORO-OMEGA-NITROSTYRENE
Tetrahydropyran
1-Methylcyclopentanol
trans-4-Benzyloxy-N-FMoc-L-proline, 98%
Bis(pentafluorophenyl) carbonate, 98+%
Isodecyl acrylate
N-(2-AcetaMido)iMinodiacetic acid
4-EthoxycarbonylMethylphenylboronic acid, pinacol ester
5,7-Difluoro-1-indanone
AluMinuM nickel isopropoxide, 99.9% (Metals basis), 10% w/v in isopropanol
Methyldiethoxyphosphine
3,4-Dimethylbenzyl chloride
Silver(II) fluoride
3-Bromo-5-ethoxy-4-hydroxy-benzonitrile
(2-Amino-thiazol-5-yl)-acetic acid methyl ester