NW38细胞|人黑色素瘤细胞系价格多少 货号:GD-C10319710
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞,说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞,就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞,肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞,刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4,6-DiMethyl-2-pyriMidinethiol
Terbutol
4-Hydroxyphenylboronic acid pinacol ester
N-AcetylethylenediaMine
4-FLUORO-3-(TRIFLUOROMETHYL)PHENYLACETIC ACID
1-HYDROXY-2-NAPHTHALDEHYDE
2-(2-Hydroxyphenyl)acetic acid, 97%
4-Benzyloxy-3-methylphenylboronic acid
1,2,3,4,5,6,7,8-Octahydroacridine
2-amino-4-ethyl-5-methylthiophene-3-carboxamide(SALTDATA: FREE)
Rhodium(III) oxide pentahydrate
3,5,5-TRIMETHYLOXAZOLIDINE-2,4-DIONE
Trifluoroacetic anhydride
(S)-(-)-N-Benzyl-1-phenylethylamine
3-AMino-4-chloropyridine
Butylated hydroxyanisole
9-Ethylcarbazole-3-carboxaldehyde N-Benzyl-N-phenylhydrazone
3-AcetylaMino-adaMantane-1-carboxylic acid
PotassiuM, 98%, chunks, in Mineral oil
DIFLUBENZURON
3,5-DiMethylbenzenethiol
1-Triphenylphosphoranylidene-2-propanone
Catalase From Micrococcus Lysodeikticus
3-Chlorothiophenol
Ethyl 2-benzylacetoacetate
1,4-Butanediol
9-FLUORENONE-4-CARBONYL CHLORIDE
Pentafluorophenylhydrazine
Cyclopentanone, 99%
2,5-Difluorobenzotrifluoride, 98%
cis-1,2-Bis(diphenylphosphino)ethylene, 97%
SodiuM 1-hexanesulfonate
1,3-BIS(4-HYDROXYBUTYL)TETRAMETHYLDISILOXANE
N-(Tetrahydro-2-oxo-3-thienyl)-acetaMide
BariuM hydroxide hydrate
1-BroMo-3-(MethoxyMethyl)benzene
4,4'-DiaMinodiphenylsulfone
Benzoyl isothiocyanate
(Isocyanoimino)triphenylphosphorane
1H,1H-Perfluoroundecan-1-ol 95+%
2-Methyl-1-phenylpropene
2-Butyl-1H-imidazole-4-carbaldehyde
2,4-DIMETHYLPHENYL ISOTHIOCYANATE
Testosterone isocaproate
2-BroMoethylaMine HydrobroMide
Allyl chloride
DerMatan Sulfate SodiuM Salt
2-Vinyl-4,6-diaMino-1,3,5-triazine
Tris(triMethylsilyl) borate
SodiuM Acetate, Trihydrate, Technical
1,3,5-TriMethylcyclohexane
Furfuryl acetate
Bis(4-aminophenoxy)ethane
Zirconium n-butoxide
2-Fluoro-4-methoxybenzoic acid
Isopropyl acetoacetate
4-Chlorobenzotrifluoride
Diuron
4,4'-BIPHENYLDISULFONIC ACID
2-(Trifluoromethyl)-1H-benzo[d]imidazole, 97%
L(+)-Tartaric acid, disodium salt, dihydrate, reagent ACS, 99+%
Chloromethyl ethyl ether, tech., stabilized, 80%
2-(4-Chlorophenyl)benzothiazole
METHYL (2R,3S)-2,3-EPOXY-3-(4-METHOXYPHENYL)PROPIONATE
4-Cyano-3-fluorobenzoic acid
N-ACETYL-DL-SERINE
N-ISOBUTYRYL-D-CYSTEINE
L-(?)-3-Phenyllactic acid
2,3,5,6-Tetrafluoro-7,7,8,8-tetracyanoquinodiMethane
TetrabutylphosphoniuM Benzotriazolate
2-Chloropropionic acid, 95%
4-(Pyrrolidin-1-ylMethyl)benzylaMine, 97%
4-Hydroxy-3,5-dimethoxybenzaldehyde azine
4-BroMo-2,6-di-tert-butylphenol
(R)-3-aminoPentanoic acid
Dithiopyr
2-methyl-1-(3-sulphonatopropyl)quinolinium
Poly(ethylene glycol)
ET-1 ELISA Kit
COL12 ELISA Kit
Human insulin-like growth factors 2,IGF-2 ELISA Kit
Human rudimental bovine serum albumin check-up ELISA Kit
Lp-a ELISA Kit
ALCAM ELISA Kit
TNFα ELISA Kit
DA ELISA Kit
Human IGF-2 ELISA Kit
Col Ⅳ ELISA Kit
B12 Assay Medium
Vogel-Johnson Agar Base
Diethylene glycol Monoethyl ether acetate, 99%
trans-1,2-Bis(diphenylphosphino)ethylene, 98%
4-Ethylphenyl trans-4-(4-n-pentylcyclohexyl)benzoate, 99%
2-AMino-6-ethylpyridine, 97%
1-Triphenylphosphoranylidene-2-propanone
Cupric Tartrate
1-N-Ethoxycarbonyl-3-pyrrolidone
Basic Violet 14
2,3,4,5-TETRAFLUOROBENZOTRIFLUORIDE
3-Acetylindole
Mes Monohydrate
3-(1H-Tetrazol-5-yl)benzyl alcohol, 96%
Diethyl di-n-propylMalonate, 97%
3,4,5-Trifluorophenylboronic acid
Ethyl 4-hydroxycyclohexanecarboxylate, Mixture of cis and trans
3,5-Difluoro-2-hydroxyphenylboronic acid
2-Chloro-5-cyanophenylboronic acid
TriMethyl 4-broMoorthobutyrate
TRIMETHYL 1,2,3-PROPANETRICARBOXYLATE
Sodium hexachloroplatinate(IV) hexahydrate, 98+%
3-Hydroxybenzoic acid ,99%
Alternariol 99%
2,4,6-Trimethyl-1,3-phenylene diisocyanate, 98%