HME1细胞|人黑色素瘤细胞系价格多少 货号:GD-C10319625
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
trans,trans-1,4-Diphenyl-1,3-butadiene
Ethyl valerate
4-CHLOROPYRIDIN-3-YLBORONIC ACID, PINACOL ESTER 98
1,6-Dihydroxynaphthalene
4-AMino-4H-1,2,4-triazole
N-(4-Methoxybenzylidene)-4-Methoxyaniline
2-Methoxynicotinic acid , 97%
Ethyl Thioglycolate
5-fluoro-1H-indole-2,3-dione(SALTDATA: FREE)
2,3,4,6-Tetra-O-benzyl-D-glucopyranosyl fluoride
2-Aminonicotinic acid
TETRAGLYCINE
4-BENZYLPHENYLACETONITRILE
Ethyl 3-(chloroformyl)propionate
L(+)-PenicillaMine
Arachidic acid
Vinyl 2-Ethylhexanoate (stabilized with MEHQ)
5-Methylpyridine-2-carboxaldehyde
3-BroMo-2-nitrotoluene, 98%
BLADEX
Furfuryl Methacrylate (stabilized with MEHQ)
Heptamethyleneimine
Iron(III) nitrate nonahydrate
3-Chlorosalicylic acid
Ethyldiphenylphosphine
4-CHLOROBENZYL METHYL SULFIDE
4'-Aminobenzo-15-crown-5-ether
3-BroMobiphenyl
Methyl (phenylthio)acetate, 99%
2-Methyl-2-pentanol, 99%
SodiuM hydrosulfide hydrate
6-Benzyloxyindole
2-Chloro-6-Methoxyquinoline-3-carboxaldehyde, 99%
N,N-DiMethyltriMethylsilylaMine
N-(2-DIMETHYLAMINOETHYL)-N-METHYLFORMAM&
5-AMino-4-cyano-1-(4-fluorophenyl)-3-Methylpyrazole
1-Iodohexane, stabilized, 98+%
Nickel(II) Benzenesulfonate Hexahydrate
3-[N-(2-Hydroxyethyl)sulphamoyl]benzeneboronic acid 98%
4-(methoxymethyl)benzoic acid(SALTDATA: FREE)
9-Fluorenone-4-carboxylic acid
2,3,4-Trifluoro-6-nitroaniline
1-Methyl-2-pyridone
L-Citrulline
2,6-Dichlorobenzothiazole
2′,3′-O-Isopropylideneguanosine
N-Phenyl-9-anthraMine
1-(3-BroMopropoxy)-2,4-dichlorobenzene, 97%
2-BroMo-4-trifluoroMethoxyaniline, 98%
Tributyl phosphate
2-(2-AMino-3-Methoxyphenyl)chroMone
2-Amino-4-phenylthiazole hydrobromide monohydrate
ethyl 2-(formylamino)-4-thiazoleacetate
Methyl 4-methoxyphenylacetate
Anhydrotetracycline hydrochloride
1-CYCLOHEXYL-1-PROPANOL
9,10-DiMethoxy-2-anthracenesulfonic acid sodiuM salt Monohydrate
LithiuM benzoate
4-BroMo-3-cyanopyridine
Bromine - 1,4-Dioxane Complex
Quinazoline ,98%
Manumycin A, Streptomyces parvulus
N,N-Diethylformamide, 99%
1-(3-NITROPHENYL)-
INDOLE-3-BUTYRIC ACID POTASSIUM SALT
DIETHYL 4-CHLOROBENZYLPHOSPHONATE
Dibenzo-18-crown-6
4-MorpholinobenzenecarbothioaMide
2,2,6,6-TetraMethyl-3,5-heptanedione
(1S,4S,5S)-4,7,7-TriMethyl-6-thiabicyclo[3.2.1]octane
2-Methyl-2-pentenal, 97%
p-Phenetidine, 98%
3-Hydroxy-2-methyl-4H-pyran-4-one
2-BroMoethanol
Di(1-adamantyl)benzylphosphine
2-Aminoethylmethylsulfone hydrochloride
diethylmalonyl dichloride
Isomannide
CIAP ELISA Kit
Mouse CEBPD (CCAAT/Enhancer Binding Protein Delta) ELISA Kit
Human ghcagons-like pepfide 1,GLP-1 ELISA Kit
Human ETFDH ELISA Kit
TCF20 ELISA Kit
IL4 ELISA Kit
IL-1β ELISA Kit
Human Azidothymidine,AZT ELISA Kit
Human nitric oxide synthase,NOS ELISA Kit
HSPA8 ELISA Kit
Nodule Bacteria Medium Ⅱ Base
Cellulose Decomposing Microorganisms Medium
Ethyl 5-aMinoiMidazo[1,2-a]pyridine-2-carboxylate hydrate, 95%
trans-4-Octene, 97%
4'-Acetoxyacetophenone, 99%
2-AMinoiMidazole Sulfate
Heptamethyleneimine
2-Methyl-3-butenenitrile
1,4-butanediol bis(3-aminocrotonate)
4-Ethylbenzoic acid
1-(2-Thienyl)-1-propanone
Titanocene dichloride
GadoliniuM(III) hydroxide hydrate, 99.9% (REO)
Methyl benzo[b]thiophene-2-carboxylate, 98%
2,5-Difluorophenylacetonitrile, 97%
4-(benzyloxy)-2-fluorophenylboronic acid
ForMaldehyde SodiuM Bisulfite HeMihydrate
4′-Hydroxy-3′,5′-diMethylacetophenone
RONGALITE
4-Methyl-1-tetralone
2-(2-PYRIDYL)BENZIMIDAZOLE
4-(4-Ethylphenyl)-4-oxobutanoic acid, 97%
(4-methyl-1,3-thiazol-5-yl)methanol(SALTDATA: FREE)
5-Methyl-2-(4-nitrophenyl)-2H-pyrazol-3-ylamine
2,4-dibromobenzathiazole