TU212细胞|人喉癌细胞系多少钱 货号:GD-C10118298
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-[Ethyl(4-Methoxybenzyl)sulfaMoyl]benzeneboronic acid, 98%
1-Fluoro-2-(trifluoroMethoxy)benzene
2,5-Bis(trifluoroMethyl)phenylboronic acid
Chlorhexidine acetate
SodiuM acetate trihydrate
Zidovudine
5-Ethoxy-2-fluorophenylboronic acid
Glycylglycylglycine
3-(2-methylphenoxy)propanoic acid(SALTDATA: FREE)
n-Butyl vinyl ether, stabilized, 98%
4-Chloro-4'-methylbenzophenone
Diethylene glycol diacrylate
3-Fluorobenzoyl chloride
(R)-(-)-2-Methyl
4-(Chloromethyl)benzoic acid
Tin(II) fluoride
4,4'-DiaMinooctafluorobiphenyl
1-BroMo-4-Methoxynaphthalene
PolyethyleneiMine, approx. M.N. 60,000, 50 wt% aq. solution, branched
Fluorescein, Alcohol Soluble
5-Methyl-2-furaldehyde
sec-Butylbenzene
Vinyl sulfonate, Sodium salt
1,3,5-Triphenylformazan
1,1,2-tribromoethane
Poly(3-octylthiophene-2,5-diyl)
4-Amino-1-benzylpiperidine
3-BroMo-4-chlorobenzoic acid
HolMiuM(III) sulfate hydrate, 99.99%
4-tert-Butylcyclohexanecarboxylic acid, predoMinantly trans, 98+%
Benzoic acid
4-Ethylbenzoyl chloride
3-Methyl-1,5-pentanediol
Z-D-MET-OH
PHENYLTHIOHYDANTOIN-GLUTAMINE
LincoMycin Hydrochloride Monohydrate
2-Hydroxy-4-(methacryloyloxy)benzophenone ,99%
3-Cyclopropyl-1-methyl-1H-pyrazol-5-amine , 97%
Quinoline-8-carboxylic acid
Methyl 2-Oxovalerate
2,2-DIMETHYLPROPANE
5-Fluoro-2-nitroaniline
3-Chloropropylmethyldimethoxysilane
Dierbium trioxide
4-Nitrobenzonitrile
DecylaMine
N-MethyldodecanaMide
FMoc-2-iodo-l-phenylalanine
2-BroMo-1-(5-Methyl-3-phenylisoxazol-4-yl)ethan-1-one, 97%
ZIRCONIUM
L-Phenylephrine Bitartrate
5,7-dihydroxy-6-[(2S,3S,4R,5R,6R)-3,4,5-trihydroxy-6-(hydrox...
4-n-Hexyloxyaniline
Methylpentenal
Ethyl 2-methylthiazole-4-carboxylate
1,2,3,4-TETRAPHENYL-1,3-CYCLOPENTADIENE
1-Oxa-4-azacyclohexane
FMoc-His(Bzl)-OH
Ethyl 4-borono-3-chlorobenzoate
4-Benzyloxyindole
3-Bromo-6-chloro-imidazo[1,2-b]pyridazine
Methyl cis-1-Boc-4-hydroxy-D-prolinate
Diethyl propylmalonate, 99%
(1H-benzimidazol-2-ylmethyl)methylamine(SALTDATA: 2HCl)
2,4,6-TRIPHENYLPYRYLIUM TETRAFLUOROBORATE
3-METHYLTHIOPHENE-2-CARBONYL CHLORIDE
2-Fluoro-5-nitrobenzotrifluoride
5-Fluoro-2-methylbenzoic acid
(+)-Diisopropyl L-tartrate
1,8-Dichlorohexadecafluorooctane
N-t-Butyl 3-broMo-4-MethoxybenzaMide
Tetrakis(triphenyl phosphite)nickel(0), 95%
Nile Blue Chloride
Acrolein Diethyl Acetal
butyl nitrite
dimethyl methoxymalonate
1,4-Dichloro-2-butene
2,6-diaminoanthraquinone
AIRE ELISA Kit
CFB ELISA Kit
Human CTSH ELISA Kit
Human Osteoprotegerin,OPG ELISA Kit
HSPB1 ELISA Kit
Hamster receptor activator of nuclear factor kappa B ligand,RANKL ELISA Kit
HbH ELISA Kit
Human GAL8 ELISA Kit
Human Cytochrome P450c21/21-hydroxylase,CYP21B ELISA Kit
SEPCR ELISA Kit
Veal Infusion Agar
V-KM Medium
LARD
NAPHTHOL YELLOW S
5-BroMo-2,3'-bipyridine, 95%
2'-Hydroxyacetophenone, 98%
sec-Butylbenzene
Succinic acid, disodium salt hexahydrate
(6-Chloro-2-pyridyl)hydrazine
DL-Pantolactone
2-CHLORO-4-IODO-NICOTINIC ACID
PHENYLGLYOXAL MONOHYDRATE
TerbiuM(III) acetate hydrate, 99.99%
7-Ethylindole, 98+%
Vinylcyclohexane, 97%
1,6-Anhydro-β-D-glucose
N-Methyl-1-adaMantylaMine
3'-HYDROXYFLAVANONE
4-Pyridoxic acid
4-NITROANILINE HYDROCHLORIDE
2-Chlorophenyl cyclopentyl ketone, 97%
6-(Bromomethyl)-1,1,4,4-tetramethyl-1,2,3,4-tetrahydronaphthalene , 90%
5-Bromo-1-methyl-2-oxoindoline, 97%
S-(-)-Cotinine
2,2-Dimethyl-3-hexanol