PC-9细胞|人肺癌细胞系多少钱 货号:GD-C10118288
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-[2-(TrifluoroMethyl)phenylcarbaMoyl]benzeneboronic acid, 98%
4-AMinoMethyl-1-Methylpyrazole
2-Methylbenzyl chloride
2,6,7-Trichloro-3-trifluoroMethylquinoxaline
IODOMETHYLTRIMETHYLAMMONIUM IODIDE
CIS-6-CHLORO-2-HEXENE
BIS(4-HYDROXY-3-METHYLPHENYL) SULFIDE
2,1,3-Benzothiadiazole-4-sulfonyl chloride, 97%
1,2,3-Butanetriol
2,6-Dichloro-4-(trifluoromethyl)phenylhydrazine 98%
2'-Dicyclohexylphosphino-2,6-di-i-propyl-4-sulfonato-1,1'-biphenyl hydrate sodium salt
4-Hydroxybenzyl alcohol
6-Thioguanine
1-Amino anthraquinone
1,6-Dibromo-2-naphthol
1-BroMonaphthalene
1,1'-Diheptyl-4,4'-bipyridiniuM DibroMide [for ElectrochroMic Material]
5-BroMo-(2-MethoxyethylaMino)pyriMidine
Poly(N-vinylcarbazole), approx. M.W. 90,000
Ferrous Gluconate, USP
Puerarin
3-hydroxy-N-2-naphthyl-2-naphthamide
4(5)-imidazolecarboxaldehyde
4-Fluoro-4'-methylbenzophenone
Trioctylphosphine oxide
Calcium bromide
CDP
PropionaMidoxiMe, 97%
Ethyltriethoxysilane, 96%
Nicorandil
N-Ethyl 5-broMo-2-MethoxybenzenesulfonaMide
Zinc Methacrylate
4-Fluoro-4'-hydroxybenzophenone
MORPHOLINOACETONITRILE
1-(5-Nitropyridin-2-yl) 95%
1H,1H,2H,3H,3H-2-Iodoperfluoroundecan-1-ol
(Z)-4-Chloro-2-methoxycarbonylmethoxyimino-3-oxobutyric acid
Gluconic Acid Iron(II) Salt Hydrate
N-(p-Tosyl)-L-phenylalaninyl chloride
Hexyl 2-methylbutyrate
4-CHLORO-6-FLUORO-2H-BENZOPYRAN-3-CARBOXALDEHYDE
4-Amino-3-nitrophenol
1-(3-Chloropropyl)theobroMine
2-Methyl-4-(N-MethylsulfaMoyl)phenylboronic acid
Ferroceneacetic acid, 98%
N-HydroxybenzenesulfonaMide, 97%
1-Iodododecane (stabilized with Copper chip)
6-BroMo-1-hexanol
Ethyl alpha-bromophenylacetate
1-Naphthaleneacetamide
4-CHLORO-7-FLUORO-6-NITRO-QUINAZOLINE
4-Methoxy-2-(trifluoromethyl)benzaldehyde
n-Octacosane
4-(PHENYLSULFONYL)-2-AZETIDINONE
3-Methylpyridine-2-boronic acid
3-THIOPHEN-2-YL--1-CARBOXYLIC ACID ETHYL ESTER
5-Aminopyrazine-2-carbonitrile
trans-Cinnamamide
5-(3-Aminophenyl)tetrazole
cis-Bis(2,2'-bipyridine)dichlororuthenium(II) hydrate, 97%
2,3,6-TRIFLUOROPHENYLACETONITRILE
4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride
Isobutyl acetate
2-Bromo-3-methylbenzoic acid
Hydroxychloroquine sulfate
(+)-BenzylMethylphenylsilylacetic Acid [for e.e. DeterMination by NMR]
5-Carboxynaphthalene-1-boronic acid
Atropine sulfate Monohydrate, 98.5%
(R)-N-Phthaloyl-2-aMinobut-3-en-1-ol, 95%, (95% ee)
(1,3-Dioxolan-2-yl)MethyltriphenylphosphoniuM BroMide
1,2,3,4,5-Pentaphenyl-1,3-cyclopentadiene
Isophthalaldehyde
tert-Butyl methyl[(3R)-piperidin-3-yl]carbamate
lomefloxacin
n-Octadecylmethyldiethoxysilane
PCX ELISA Kit
Mouse C5 (Complement Component 5) ELISA Kit
Human CTSO ELISA Kit
Human BMP-10 (Bone Morphogenetic Protein 10) ELISA Kit
5-LO/LOX ELISA Kit
IL-8/CXCL8 ELISA Kit
AGF ELISA Kit
Galectin9 ELISA Kit
Human CYP450 ELISA Kit
Lgals3 ELISA Kit
1% Dextrose Broth Medium
Nielsen Medium
Paclitaxel
Baicalein
5-BroMo-2-chloro-3-fluoropyridine, 98%
2-Hydroxychalcone, 98+%
3-hydroxy-N-2-naphthyl-2-naphthamide
Potassium citrate monohydrate
7-Chloro-3,4-dihydro-1H-benzo[e][1,4]diazepine-2,5-dione
1,1-dichloro-2,2-difluoroethylene
3,4-Dichlorobenzoyl chloride
2-Chlorocinnamic acid
2-Ethylhexyl BroMide
1,3-Dichloro-4-fluorobenzene, 99%
Methyl 3,5-dibroMobenzoate, 98%
Minocycline hydrochloride
Uracil
Glutaric acid diMethyl ester
2-(Benzyloxy)pyridine
Ethyl 6-MethyliMidazo[1,2-a]pyridine-2-carboxylate
DisodiuM hexahydroxoplatinate
4'-Benzyloxyacetophenone
2,5-Dimethyl-thiophene-3-carbaldehyde
1-(2-Bromoethoxy-4-bromobenzene), 98%
gamma-Linolenic Acid