NRK-52E细胞|大鼠肾细胞系多少钱 货号:GD-C10118280
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Carboxy-4-chlorobenzeneboronic acid, 98%
(S)-(+)-3-(Benzyloxycarbonyl)-5-oxo-4-oxazolidineacetic acid
2-DiMethylaMinoethyl chloride hydrochloride
PHENYL ISOTHIOCYANATE
2-Chloro-4-Methylbiphenyl-3-carboxylic acid
5-Carboxyphthalide
5-Nitroindazole
2,6-Dimethoxybenzonitrile 97%
2,4,6-Tris(pentadecafluoroheptyl)-1,3,5-triazine [for Mass spectrometry]
3-Chloro-4-methoxyacetophenone
4,4'-Dinitrobiphenyl
1-BROMO-2-NAPHTHALDEHYDE
N-Boc-L-alaninol
2-(DIFLUOROMETHOXY)BENZOIC ACID
Hexamethylphosphorous triamide
4-Chloro-4'-fluorobutyrophenone
Diisobutyl Ether
3-BroMo-5-(4-Methoxybenzyloxy)pyridine
Poly(caprolactone) diol, average M.N. 2000
Ferric AMMoniuM Sulfate, Dodecahydrate, Reagent, ACS
4-tert-Butoxybenzaldehyde
3-hydroxy-2,7-naphthalenedisulfonic acid, disodium salt
2-Amino-5-bromothiazole
Myricetin
tris(p-tert-butylphenyl) phosphate
4-Chloro-1,8-naphthalic anhydride
1-Octyn-3-ol
2-Naphthol-3,6-Disulfonic Acid DisodiuM Salt
N-FMoc-3-cyclohexyl-D-alanine, 96%
4'-BroMo-2'-ethylacetanilide, 97%
5-BroMoindole
SodiuM azide
2-ISOPROPYL-2-OXAZOLINE
ZINC TERT-BUTOXIDE
N-Cbz-L-Serine
5-ForMylfuran-2-boronic acid, neopentyl glycol ester
Methyl 3,5-difluorobenzoate
3,3,3-Trifluoropropane-1-sulfonyl chloride
3'-Chloro-2,2,2-trifluoroacetophenone
3-(1,3-Thiazol-2-ylcarbamoyl)benzeneboronic acid 97%
(METHOXYMETHYL)TRIMETHYLSILANE
Fingolimod hydrochloride
N-(3-Aminopropyl)morpholine
Methyl carbazate
3-(1H-1,2,4-Triazol-1-ylMethyl)aniline
Bis(benzonitrile)dichloroplatinuM(II)
2-Methyl-2-hexanol
Methyl 5-hydroxypyrazine-2-carboxylate
DiMethyl 4-(4-Methoxyphenyl)-2,6-diMethyl-1,4-dihydropyridine-3,5-dicarboxylate, 97%
Roxithromycin
Tetradecylcyclohexane
Ethiodized oil
Polypropylene glycol 2000 dimethacrylate
4-(chlorophenylmethyl)-1,1'-biphenyl
Cyclopropyl phenyl ketone
1-N-BUTYLCYCLOHEXANOL
4-(3-Pyridinyl)benzaldehyde
PYRITE
4-AcetaMido-3-(trifluoroMethy)phenylboronic acid, pinacol ester
8-Amino-2-naphthol
3,4,5-Trimethoxybenzyl alcohol
Bis(2-bromoethyl) ether (stabilized with water)
(S)-(+)-1-(1-Naphthyl)ethyl Isocyanate
1,5,5-TRIMETHYLHYDANTOIN
3-Ethyl-2-hydroxy-2-cyclopenten-1-one
N,N-Dimethyl-1,4-phenylenediamine
4-Hydroxyquinazoline
4-Fluorothioanisole
(+)-10-CaMphorsulfoniMine
4-Pentenylboronic acid
Guanosine 5'-triphosphate, disodiuM salt hydrate, 90%, for biocheMistry
3-Chloro-2-(chloroMethyl)-5-(trifluoroMethyl)pyridine, 95%
2-AMino-5-Methylbenzoic Acid
1-Phenyl-5-pyrazolone-3-carboxylic Acid
2-[4-(dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
2-Amino-6-chlorobenzothiazole
Ambrisentan
2-aminosulfonyl-N,N-dimethylnicotinamide
HDAC ELISA Kit
PAT ELISA Kit
Human Cath Ab ELISA Kit
Human Bone morphogenetic protein 2,BMP-2 ELISA Kit
HSPA4 ELISA Kit
KISS1 ELISA Kit
VEGF-B ELISA Kit
Human GAL4 (Galectin 4) ELISA Kit
Human Cytochrome-C,Cyt-C ELISA Kit
Ses ELISA Kit
AC Broth
NS medium
Carnauba wax
Chalcopyrite, naturally occurring Mineral, grains, approxiMately 0.06-0.19in
5-BroMo-2-Chlorophenol
2-HydroxyMethyl-18-crown-6, 97%
3-hydroxy-2,7-naphthalenedisulfonic acid, disodium salt
2H-1-Benzopyran-2-one,6-hydroxy-
2-Pyrrolidinone,5,5-dimethyl-
peracetic acid
6,7-Dimethoxy-1-tetralone
BERBERINE CHLORIDE
Ethyl 2-AMinopropanoate Hydrochloride
4-AMinobutyraldehyde diMethyl acetal, 98+%
Phenyl broMoacetate, 98%
Butyryl chloride
Benfluorex hydrochloride
3'-Acetoxyacetophenone
O-(Tetrahydropyran-2-yl)hydroxylaMine , 98%
5-BroMopyridine-3-sulfonic acid
trans-4-MethoxycinnaMoyl chloride
2-Acetylcyclohexanone
Boc-D-Arg(Tos)-OH
3,6-Difluoro-2-hydroxybenzaldehyde
(S)-(-)-1,1,2-Triphenyl-1,2-ethanediol