M109细胞|小鼠肺癌细胞系多少钱 货号:GD-C10118266
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-(3-Piperidinyl)azepane, tech. 90%
(S)-Glycidyl 3-Nitrobenzenesulfonate
5-Methylhexan-1-ol
5-INDANOL
(S,S)-DACH-naphthyl Trost Ligand
BENZAL DIACETATE
2-ISOBUTYL-3-METHYLPYRAZINE
Undecylenic acid, 99%
Tetrakis dimethylsilylsilane
p-Xylylenediamine
3,3',4',5-Tetrachlorosalicylanilide, 99%
Hydroxypropyl methyl cellulose
3,3-BIS(METHYLTHIO)-2-[3-(TRIFLUOROMETHYL)BENZOYL]ACRYLONITRILE
Diethyl propylmalonate
5-Chlorovaleryl chloride
1-[3-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)benzyl]pyrrolidine
Diethylene Glycol Dibenzoate
4-BroMo-3-MethoxybenzaMide
PlatinuM(II) acetylacetonate, 98%
FD and C Green No. 3
cis-1,4-Cyclohexanediol
4-Chlorobenzophenone
2-Pyridinepropanol
Citraconic acid
Yohimbine hydrochloride
Diisobutyldimethoxysilane
N-[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)PHENYL]SUCCINAMIC ACID
4-Chloro-4-Hydroxybenzophenone
3-BroMo-2-chloro-6-Methylpyridine, 98%
2-Methyl-6-nitroquinoline, 98%
2,3,4,5,6-Pentafluorobenzyl broMide
Chlorodifluoroacetic acid
4-(2-AMinoethyl)-1-benzylpiperidine , 98%
Z-Lys-ONp hydrochloride
Diisopropyl(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)silane
4-Iodo-2-nitrotoluene
6-Amino-4-methyl-2H-1,4-benzoxazin-3(4H)-one
Thieno[2,3-b]pyridine-2-carbaldehyde, 97%
(2-Chloroethyl)trimethylammonium chloride, 98%
Biocytin Hydrazide hydrochloride
Sodium fluoroaluminate
3-Chlorobenzyl cyanide
3-ACETYLPYRROLE
1-Fluoro-2-nitrobenzene
2-Fluoro-5-(trifluoroMethyl)phenyl isocyanate
2-(Phenylthio)aniline
1,1,2-TriMethoxyethane
2,2,2-TrifluoroacetaMide, 97%
Bis(4-tert-butylphenyl)iodoniuM triflate
1,2,3,4-TETRAHYDROISOQUINOLINE
3-MethylthiopropylaMine
1,2-Bis(2-aminophenoxy)-ethane-N,N,N'N'-tetraacetic acid
4-Benzyloxy-piperidine Hydrochloride
Gonadorelin acetate
2-Bromo Triphenylene
(S)-N-Fmoc-(3-Pyridyl)alanine
1-Chloro-3-fluorobenzene
(R,R)-(-)-N,N'-BIS(3,5-DI-TERT-BUTYLSALICYLIDENE)-1,2-CYCLOHEXANEDIAMINE
2-(CHLOROMETHYL)ALLYLTRICHLOROSILANE
4-Iodo-1-methyl-1H-pyrazole
7-Bromo-2-methyl-2H-indazole
2-Chloro-4-methoxybenzoic acid
Tetraethylene Glycol Bis(p-toluenesulfonate)
TRIETHYLENE GLYCOL MONOBUTYL ETHER
Methyl 2-formyl-4-thiophenecarboxylate
Propyl propionate
HEXAHYDRATE
2-BroMo-1-(2-chloropyridin-4-yl)ethanone
(-)-Carveol, Mixture of isoMers
MethoxyMethyl Phenyl Sulfide
5,6-Difluoroindole
2-AMino-5-chlorobenzonitrile, 97%
Chelidamic acid
Diethyl Allylphosphonate
Calcium nitrate tetrahydrate
2-(bromomethyl)pyridine hydrobromide
N-Cyanoimido-S,S-dimethyl-dithiocarbonate,remainder water
1,3,5-BENZENETRIMETHANOL
TIMP1 ELISA Kit
FoxP3 ELISA Kit
TIMP1 ELISA Kit
BMPR1A ELISA Kit
COL1α2 ELISA Kit
CKM ELISA Kit
AGT ELISA Kit
Human GAL12 (Galectin 12 ) ELISA Kit
Human CYB (Cytochrome b5) ELISA Kit
Gzms-B ELISA Kit
Nutrient Agar with TTC
Tryptone Yeast Cystine Sucrose Bacitracin Medium
Isopropyl palMitate, tech. 85%
SulfacetaMide, 98%
5-BroMo-2-chloropyridine-4-boronic acid, 96%
2-Hydroxynicotinic acid, 99%
4-Chlorobenzophenone
N-Benzyloxycarbonyl-D-serine
5-hydroxyisoquinolin-1(2H)-one
propionyl chloride
1,4-Diaminobutane dihydrochloride
BIS(ETHYLCYCLOPENTADIENYL)VANADIUM
5-Chlorosalicylic Acid
4-AMino-6-hydroxypyriMidine, 98%
4-n-Nonylaniline, 98%
1-Acetylnaphthalene, 97+%
Bis(4-chlorophenyl) disulfide
4-DIBENZYL 1-NAPHTHYL KETONE
9,10,12,13-TETRABROMOSTEARIC ACID
2-BroMothiophene
2-Ethyl anthraquinone
2,3-NAPHTHALENEDICARBOXYLIC ACID
2-(1,8-Naphthyridin-2-yl)phenol
1-Vinylnaphthalene (stabilized with TBC)
2,3,4-Trifluoroanisole