SupT1细胞株|人T淋巴瘤细胞系技术优势大 货号:GD-C9920566
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl (2-Methylphenoxy)acetate, 99%
Heptacosanoic acid
Butanethiol
2,5-DiMethoxyphenylboronic acid
1-(4-BroMophenyl)-3-phenylpyrazole-4-propionic acid
4-AMino-1-naphthalenecarbonitrile
Ethyl butyrate
Benzophenone imine ,98%
Isophthalamide
2,3-Dibromopropionyl Chloride
2-({1-[3-(Trifluoromethyl)phenyl]prop-2-yl}amino)ethyl benzoate hydrochloride
N,N-Diethylacetoacetamide
3,4,5-TRIFLUOROBENZOYL CHLORIDE
2-Fluoro-3,5-dichloropyridine
N-METHYL-1,3-PROPANEDIAMINE
2-Phenylethyl acetate
Diphenylphosphinic Anhydride
3-(4-BroMo-1H-pyrazol-1-yl)-6-chloropyridazine
Stearic anhydride, 98%
Megestrol Acetate, Micronized, USP
1-AMino-2-Methyl-2-propanol
Oryzanol
4-nitrobenzene-1,3-dicarboxylic acid
1,4-Cyclohexadiene
Di-tert-butyl peroxide
2-(HYDROXYMETHYL)ANTHRAQUINONE
Dibenzo-18-crown-6
6-Methoxyisoquinoline
Copper(II) oxide
N,N-DiMethylhexylaMine, 99%
Tri-n-propyltin chloride
SodiuM acetate-d3
2,2-Bis(hydroxyMethyl)butyric acid
tert-Butyl Methacrylate
BARIUM BROMIDE
3-Ethyl-o-xylene , 99%
4-Cyanoimidazole
1,2-Bis(2-benzylaminoethoxy)ethane
5-Bromo-1,3-dimethyl-1H-pyrimidine-2,4-dione
5-Chloro-2-methoxypyridine-3-boronic acid pinacol ester
Triacetonediamine
H-7 DIHYDROCHLORIDE
4-(4-CHLOROPHENYL)-3-THIOSEMICARBAZIDE
beta-D-Ribofuranose 1-acetate 2,3,5-tribenzoate
2-(2-Methoxyethoxy)ethanol
3-Chloro-2-chloroMethyl-1-propene
2-Fluoro-5-MethyldiphenylaMine
3-AMino-4-fluorobenzoic acid, HCl
2,3,6-Trifluorophenylacetic acid, 98%
2,6-Dihydroxypurine
Ethyl all cis-5,8,11,14,17-Eicosapentaenoate (stabilized with Tocopherols)
Benzene, 1-bromo-4-(phenylmethyl)-
(trimethylsilyl)methylmagnesium chloride
Ethyl alpha-bromophenylacetate
3,5-dimethoxybenzyl bromide
3,4,6-Tri-O-acetyl-D-galactal
2-Chloro-5-boronobenzaMide
4-Nitrobenzyl broMide
1-Phenyl-1,3-butanedione
4-BroMo-3-Methylacetanilide, 98%
2-Aminofluorene
4-(Difluoromethoxy)benzyl alcohol 98%
3-Buten-2-yl Acetate
3-Amino-3-(3,4-dichlorophenyl)propanoic acid
N-Methyldiethanolamine
Ketorolac
1,3-Dibromobutane
THIOBENZAMIDE
1-(2-Phenyl-1,3-thiazol-4-yl)ethan-1-one
Methyl 3-(DiMethylaMino)benzoate
6-BroMo-3-(Methylthio)-[1,2,4]triazolo[4,3-a]pyridine
Reinecke salt, ACS reagent
Iron AtoMic Absorption Standard
N-(tert-Butoxycarbonyl)nortropinone
2,2'-Bis(trifluoromethyl)-4,4'-diaminodiphenyl ether
1-ethyl-3-chloro-Tetrahydropyrrole
Triacontanol
1,2,3-Tribromopropane
TAP ELISA Kit
IL-23 ELISA Kit
Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA Kit
Human PHC ELISA Kit
Ub ELISA Kit
Rat Cluster of differentiation 30,CD30 ELISA Kit
Aβ1-42 ELISA Kit
Human HNRPK ELISA Kit
Human CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit
NRG-1 ELISA Kit
Hektoen Enteric Agar Medium
NGKG Agar Base
Triethyl orthoacetate, 98%
Tert-Butyl Peroxy-2-Ethylhexanoate
5-Methyl-1,10-phenanthroline, 99%
Ethyl 2-Methylthiazole-4-Carboxylate
Oryzanol
Cinnamic acid propyl ester
Phthalimide acetyl chloride
4-methyl-2-nitroanisole
4-Hexyl-1,3-benzenediol
1,3-Dioxolane, Stabilized
4-Methoxy-4-Methyl-2-pentanone, 97%
TriMethyl 1,3,5-benzenetricarboxylate, 98%
Ethyl 3-ethoxypropionate, 99+%
Methyl crotonate
1-Trityl-1H-iMidazole-4-carboxylic acid
1-(3,4-Dichlorobenzyl)
3-Chloro-1-phenyl-2-propen-1-one
(R)-[(RuCl(H8-BINAP))2(μ-Cl)3][NH2Me2]
Ethyl 5-broMofuran-2-carboxylate
3-Propylidenephthalide
Methyl 4'-(bromomethyl)biphenyl-2-carboxylate ,98%
4-(5-isopropyl-1,2,4-oxadiazol-3-yl)benzoic acid(SALTDATA: FREE)
3-Methylnonane