MA-782细胞株|小鼠乳腺癌细胞系技术优势大 货号:GD-C9919680
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Chlorophenyl cyclopentyl Ketone
4-Fluorophthalic anhydride
Methyl 1-(2-nitro-4-trifluoroMethylphenyl)cyclopropanecarboxylate
1-(4-BroMo-2-fluorobenzyl)
Bis(4-fluorophenyl)phenylphosphine oxide
7-Benzoylheptanoic acid, 98+%
Trichloroacetyl Isocyanate
2-Tetrahydropyran-4-ylethanol , 97%
4-Morpholin-4-ylbenzylamine
3-Fluoro-5-isobutoxybenzeneboronic acid 98%
6-METHYL-2-PYRIDINEMETHANOL
N-BENZYLCINCHONINIUM CHLORIDE
5-(2-Chloroethyl)tetrazole
N-(4-Methylphenyl)-3-oxobutanamide
D-Methionine
3-[4-(BroMoMethyl)phenyl]-5-Methyl-1,2,4-oxadiazole
2-Ethoxy-1-naphthoyl Chloride
1-AcetaMido-4-broMonaphthalene
5-Phenyl-1-pentanol, 98%
DECAFLUOROTRIPHENYLPHOSPHINE
p-Hydrazinobenzenesulfonic Acid HeMihydrate
mesterolone
Sodium Ligninsulfonate
2,6-Dibromopyridine-4-carboxylic acid
4-Ethylbenzoic acid
1-Propylpyridinium bromide
N-Methyl-4-pyridinamine
SodiuM Sulfite
Manganese(IV) oxide, 98%
Tetra-n-hexylaMMoniuM broMide, 98%
3-Fluoro-5-(trifluoroMethyl)benzyl alcohol
4-Isopropylphenylhydrazine hydrochloride
2-Chloro-4,6-diMethoxy-1,3,5-triazine
N,N-Bis[2-(p-tolylsulfonyloxy)ethyl]-p-toluenesulfonaMide
3,4-Dichlorobenzyl broMide
1,1,2,2,3,3,4-HEPTAFLUOROCYCLOPENTANE
Calcium thioglycolate trihydrate, 99%
Trihexyl Phosphite
m-Diethynylbenzene
Diphenylchlorosilane
N-(1-Cyclohexen-1-yl)morpholine
Ethyl 5-bromovalerate
4-Nitro-1H-indazole
1,2,4-Benzenetricarboxylic anhydride
6-Nitrophthalide
HexadecyltriMethylaMMoniuM chloride
5-Fluoro-2-nitrobenzotrifluoride
3,5-Dichloro-2-pyridinecarboxylic acid
3-(Pyrrolidin-1-ylMethyl)aniline, 97%
Ethyl potassium malonate
1,3-Bis(hydroxyMethyl)urea
Aminoguanidine Hydrochloride
Reactive Black 8
allylmagnesium chloride
diethyl 1,4-cyclohexanedione-2,5-dicarboxylate
2-(3-Chloropropyl)-2-(4-fluorophenyl)-1,3-dioxolane
POLY(PERFLUOROPROPYLENE OXIDE)
2,6-DIBROMO-4-FLUOROPHENOL
P-TOLYLTRIMETHOXYSILANE
N-BOC-4-Piperidinecarboxylic acid, 98%
(5-Methyl-3-trifluoromethylpyrazol-1-yl)acetic acid ethyl ester
3,5 Dibromo-1-trimethylsilylbenzene
4,4,4-Trifluoro-1-(pyridine-3-yl)-1,3-butanedione
3-Acetyl-2-benzoxazolinone
N-METHYLSUCCINIMIDE
3-(4-Chlorophenyl)glutaramic acid
Trisodium 1,3,6-naphthalenetrisulfonate
4-Ethoxybenzaldehyde
2,2-Difluorobutyric acid
FaMotidine
(1S,3R)-3-aMinocyclopentane-1-carboxylic acid HCl
5-Phenyl-2-thienylboronic acid, 95%, May contain varying aMounts of anhydride
Dichloroacetyl chloride
4-ForMylphenylboronic Acid (contains varying aMounts of Anhydride)
4-bromo-2,5-dichlorophenol
Reactive Violet 8
Acadesine
Benzenesulfinic acid,sodium salt
NADPH/CoII/TPN ELISA Kit
BAK1 ELISA Kit
Human insulin-like growth factors binding protein 4,IGFBP-4 ELISA Kit
Human LDLR ELISA Kit
TNFaR ELISA Kit
Rat leukotriene E4,LT-E4 ELISA Kit
LDH ELISA Kit
Human TEP1 (Telomerase Associated Protein 1) ELISA Kit
IRS-β ELISA Kit
Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit
Charcoal Yeast Extract Agar(Legionella CYE Agar Ba
Actinomycete Isolation Agar
Ethyl 1-Methyl-3-trifluoroMethyl-1H-pyrazole-4-carboxylate, 97%
CIS-2-HEXENE
4-Ethylbiphenyl, 99%
ETHYL ANTHRANILATE
mesterolone
(Acetic anhydride)-d6
Chrysanthemoyl chloride
1-bromo-2,2-dimethylpropane
2-Chloro-6-fluorotoluene
Bis(ethylcyclopentadienyl)zirconium dichloride
1-ButylpyridiniuM Hexafluorophosphate
4-Allylanisole, 98%
2-Butyne, 98%
Tetracosane
FMoc-Tyr-OH
DiMethyl suberate
6-Chloro-5-nitroquinoline
1-Methyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole
2,4-Difluorophenyl isocyanate
Hydroxypropyl Methacrylate (mixture of 2-Hydroxypropyl and 2-Hydroxy-1-methylethyl Methacrylate) (stabilized with MEHQ)
Scandium(III) acetate hydrate (99.9%-Sc) (REO)
Isopropanolamine Phosphate
4-Morpholin-4-yl-butan-2-one